Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	265,792	G→A	S99F (TCC→TTC)	ykfB ←	CP4‑6 prophage; protein YkfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	265,792	0	G	A	100.0%	281.5 / NA	80	S99F (TCC→TTC)	ykfB	CP4‑6 prophage; protein YkfB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/47);  total (33/47)

TTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCC  >  NC_000913/265656‑265924                                                                                                                                         |                                                                                                                                     tttCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACg                                                                                                                                   <  1:625318/140‑1 (MQ=255)   tctcAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGa                                                                                                                                 <  2:481594/140‑1 (MQ=255)   tctcAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGa                                                                                                                                 <  1:751139/140‑1 (MQ=255)   tctcAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGa                                                                                                                                 >  1:541674/1‑140 (MQ=255)   tctcAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGa                                                                                                                                 >  1:513553/1‑140 (MQ=255)       aGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGaataa                                                                                                                             <  1:166553/140‑1 (MQ=255)         cGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATg                                                                                                                           <  1:405895/140‑1 (MQ=255)           ttcttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTa                                                                                                                         >  1:835165/1‑140 (MQ=255)           ttcttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTa                                                                                                                         <  2:844279/140‑1 (MQ=255)            tcttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTAc                                                                                                                        <  1:432807/140‑1 (MQ=255)             cttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTAct                                                                                                                       <  2:541674/140‑1 (MQ=255)             cttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTAct                                                                                                                       <  1:654786/140‑1 (MQ=255)             cttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTAct                                                                                                                       <  1:30800/140‑1 (MQ=255)              ttcCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTActg                                                                                                                      <  1:531295/140‑1 (MQ=255)                   gTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGt                                                                                                                 <  1:281079/140‑1 (MQ=255)                      cGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGAt                                                                                                              >  1:553821/1‑140 (MQ=255)                       gCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATc                                                                                                             <  2:567450/140‑1 (MQ=255)                        cAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTa                                                                                                                         <  1:794977/127‑1 (MQ=255)                        cAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTa                                                                                                                         >  2:794977/1‑127 (MQ=255)                           tGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATc                                                                                                         <  2:1208403/140‑1 (MQ=255)                           tGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATc                                                                                                         <  1:266975/140‑1 (MQ=255)                           tGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATc                                                                                                         <  2:112075/140‑1 (MQ=255)                             aaGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACCGCTGGTGATCCATCAc                                                                                                       >  1:627323/1‑140 (MQ=255)                                gTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTAc                                                                                                    <  2:1077368/140‑1 (MQ=255)                                gTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTAc                                                                                                    <  2:1065008/140‑1 (MQ=255)                                gTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTAc                                                                                                    <  2:585542/140‑1 (MQ=255)                                gTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTAc                                                                                                    <  2:553821/140‑1 (MQ=255)                                   ccTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACt                                                                                                      <  1:1118615/135‑1 (MQ=255)                                   ccTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACt                                                                                                      >  2:1118615/1‑135 (MQ=255)                                   ccTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGt                                                                                                 <  2:15137/140‑1 (MQ=255)                                     tGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGa                                                                                               >  1:724018/1‑140 (MQ=255)                                           gtgGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACt                                                                                                      >  1:879081/1‑127 (MQ=255)                                           gtgGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGCCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACt                                                                                                      <  2:879081/127‑1 (MQ=255)                                             ggTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAgtg                                                                                       <  1:200111/140‑1 (MQ=255)                                                           aCGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGa                                                                         <  2:835165/140‑1 (MQ=255)                                                           aCGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGa                                                                         >  1:839428/1‑140 (MQ=255)                                                             gTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAAc                                                                       <  1:450755/140‑1 (MQ=255)                                                             gTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTAATCGTGCGAAc                                                                       <  1:429785/140‑1 (MQ=255)                                                               cGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTg                                                                     >  1:1208958/1‑140 (MQ=255)                                                               cGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTg                                                                     >  2:1144514/1‑140 (MQ=255)                                                               cGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTg                                                                     >  1:798985/1‑140 (MQ=255)                                                               cGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTg                                                                     >  1:209621/1‑140 (MQ=255)                                                                 aaCTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGAc                                                                   >  2:625612/1‑140 (MQ=255)                                                                  aCTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGAcc                                                                  <  1:352838/140‑1 (MQ=255)                                                                     gCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATc                                                               <  2:750821/140‑1 (MQ=255)                                                                       ccGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGa                                                                    <  1:681761/133‑1 (MQ=255)                                                                       ccGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGa                                                                    >  2:681761/1‑133 (MQ=255)                                                                          gCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTc                                                          <  1:625612/140‑1 (MQ=255)                                                                           ccTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATc                                                               <  1:516003/134‑1 (MQ=255)                                                                           ccTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATc                                                               >  2:516003/1‑134 (MQ=255)                                                                                gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGc                                                    <  2:839428/140‑1 (MQ=255)                                                                                gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGc                                                    <  1:1255394/140‑1 (MQ=255)                                                                                gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGc                                                    <  1:96766/140‑1 (MQ=255)                                                                                gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGc                                                    >  2:593852/1‑140 (MQ=255)                                                                                 agaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCa                                                   <  1:593852/140‑1 (MQ=255)                                                                                     atTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAggg                                               >  1:996469/1‑140 (MQ=255)                                                                                           gatgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaagaa                                         >  1:713676/1‑140 (MQ=255)                                                                                           gatgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaagaa                                         >  2:838014/1‑140 (MQ=255)                                                                                             tgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaagaaag                                       >  2:877129/1‑140 (MQ=255)                                                                                             tgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaagaaag                                       >  1:10580/1‑140 (MQ=255)                                                                                                   cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGaa                                 <  2:798985/140‑1 (MQ=255)                                                                                                   cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGaa                                 >  1:1075192/1‑140 (MQ=255)                                                                                                   cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGaa                                 >  2:1176446/1‑140 (MQ=255)                                                                                                      aCCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGAc                                     <  2:675012/133‑1 (MQ=255)                                                                                                      aCCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGAc                                     >  1:675012/1‑133 (MQ=255)                                                                                                          ggCGTTATCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGAt                          >  2:900101/1‑140 (MQ=255)                                                                                                                aTCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGa                           <  1:766793/133‑1 (MQ=255)                                                                                                                aTCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGa                           >  2:766793/1‑133 (MQ=255)                                                                                                                aTCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAAc                    >  2:543178/1‑140 (MQ=255)                                                                                                                aTCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAAc                    >  2:779331/1‑140 (MQ=255)                                                                                                                aTCGCCGTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACGGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAAc                    >  2:1132869/1‑140 (MQ=255)                                                                                                                    ccGTTACGCCAGCCCAGGCTGAACGGAATAATGTACCGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCAtt                <  2:627323/140‑1 (MQ=255)                                                                                                                      gTTACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCt              <  2:713676/140‑1 (MQ=255)                                                                                                                        tACGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTgg            <  1:543178/140‑1 (MQ=255)                                                                                                                          cGCCAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTc          <  1:679117/140‑1 (MQ=255)                                                                                                                             cAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCgg       <  2:1075192/140‑1 (MQ=255)                                                                                                                             cAGCCCAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCgg       <  2:513553/140‑1 (MQ=255)                                                                                                                                cccAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCAtt                >  2:507734/1‑128 (MQ=255)                                                                                                                                cccAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCAtt                <  1:507734/128‑1 (MQ=255)                                                                                                                                  cAGGCTGAACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTcc  >  2:920163/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCC  >  NC_000913/265656‑265924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑