Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,026	T→C	V71A (GTA→GCA)	proV →	glycine betaine ABC transporter ATP binding subunit ProV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,026	0	T	C	100.0%	237.0 / NA	64	V71A (GTA→GCA)	proV	glycine betaine ABC transporter ATP binding subunit ProV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/38);  total (26/38)

AACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCTTTG  >  NC_000913/2804891‑2805160                                                                                                                                        |                                                                                                                                       aaCAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGc                                                                                                                                    >  2:1063732/1‑140 (MQ=255) aaCAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGc                                                                                                                                    >  1:404033/1‑140 (MQ=255) aaCAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGc                                                                                                                                    >  2:67376/1‑140 (MQ=255)     aGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTtc                                                                                                                                >  1:298645/1‑140 (MQ=255)      ggACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTtct                                                                                                                               >  1:810050/1‑140 (MQ=255)      ggACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTtct                                                                                                                               >  2:441340/1‑140 (MQ=255)       gACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTtctc                                                                                                                              >  1:774088/1‑140 (MQ=255)          tttCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCAc                                                                                                                                      >  2:824337/1‑129 (MQ=255)          tttCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCAc                                                                                                                                      <  1:824337/129‑1 (MQ=255)           ttCAAACGAACAAATTCTGGAAAACACTAACCTATCGCTTGGCGTAAAAGACGCAAGTCTGACCAATGAAGAACGCTGGATCTTTGTCATCATGGGATTATCCTGCTCGGCTAAATCCACAATGGCACACCTTCTCAATc                                                                                                                          >  2:1037087/1‑140 (MQ=255)            tCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCg                                                                                                                         <  1:80972/140‑1 (MQ=255)             cAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGc                                                                                                                        <  1:488983/140‑1 (MQ=255)             cAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGc                                                                                                                        <  2:828987/140‑1 (MQ=255)                 aGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGa                                                                                                                    >  1:284867/1‑140 (MQ=255)                 aGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGa                                                                                                                    <  2:810050/140‑1 (MQ=255)                   aaCAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGAtt                                                                                                                  <  1:1063732/140‑1 (MQ=255)                    aCAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTg                                                                                                                 >  1:890738/1‑140 (MQ=255)                           cTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAacccac                                                                                                          <  2:1038825/140‑1 (MQ=255)                                   aaCTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGc                                                                                                  <  2:1194553/140‑1 (MQ=255)                                        ggCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTg                                                                                             <  2:240514/140‑1 (MQ=255)                                        ggCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTg                                                                                             <  2:1192440/140‑1 (MQ=255)                                        ggCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTg                                                                                             <  2:1124058/140‑1 (MQ=255)                                        ggCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTg                                                                                             <  1:871362/140‑1 (MQ=255)                                               gCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  2:284867/140‑1 (MQ=255)                                               gCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  2:576829/140‑1 (MQ=255)                                               gCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  2:1084999/140‑1 (MQ=255)                                               gCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  1:509277/140‑1 (MQ=255)                                               gCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  1:1052747/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  2:575809/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  2:69116/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  2:298645/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  1:484362/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  2:404033/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  1:1236049/140‑1 (MQ=255)                                                cTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  1:592037/140‑1 (MQ=255)                                                  tGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATg                                                                                   >  1:749208/1‑140 (MQ=255)                                                  tGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATg                                                                                   >  2:957796/1‑140 (MQ=255)                                                   ggCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      >  2:567151/1‑136 (MQ=255)                                                   ggCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattg                                                                                      <  1:567151/136‑1 (MQ=255)                                                    gCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGgt                                                                                 <  2:611572/140‑1 (MQ=255)                                                     cGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     >  1:737632/1‑135 (MQ=255)                                                     cGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCtgattga                                                                                     <  2:737632/135‑1 (MQ=255)                                                               gCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGc                                                                      >  1:201105/1‑140 (MQ=255)                                                                    tCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCaaaa                                                                 >  1:1250085/1‑140 (MQ=255)                                                                        gCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATc                                                             >  2:1239791/1‑140 (MQ=255)                                                                             tGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACg                                                        >  2:49259/1‑140 (MQ=255)                                                                               aagaagGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGcc                                                      <  1:1132749/140‑1 (MQ=255)                                                                                 gaagGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCGGa                                                    <  1:49259/140‑1 (MQ=255)                                                                                 gaagGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGa                                                    <  1:432051/140‑1 (MQ=255)                                                                                 gaagGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGa                                                    <  2:774088/140‑1 (MQ=255)                                                                                   agGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAAc                                                  <  1:67376/140‑1 (MQ=255)                                                                                               tGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTgc                                      <  2:749208/140‑1 (MQ=255)                                                                                               tGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGCTCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTgc                                      <  2:1129680/140‑1 (MQ=255)                                                                                                    tcatGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGa                                 >  1:675931/1‑140 (MQ=255)                                                                                                     catGGGATTATCCGGCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGGGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGaa                                >  2:5514/1‑140 (MQ=255)                                                                                                                   gCTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATg                  <  1:957796/140‑1 (MQ=255)                                                                                                                    cTCGGGTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATgg                 >  2:1244975/1‑140 (MQ=255)                                                                                                                         gTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTc            >  1:205962/1‑140 (MQ=255)                                                                                                                         gTAAATCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTc            >  1:507591/1‑140 (MQ=255)                                                                                                                             aTCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGt        >  1:339519/1‑140 (MQ=255)                                                                                                                              tCCACAATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTc       >  1:533414/1‑140 (MQ=255)                                                                                                                                cacaATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCt     <  1:676676/140‑1 (MQ=255)                                                                                                                                 acaATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCtt    <  1:1239791/140‑1 (MQ=255)                                                                                                                                 acaATGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCtt    <  1:724848/140‑1 (MQ=255)                                                                                                                                   aaTGGCACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCTTTg  >  1:514733/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       AACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAAGTGCTGATTGATGGTGTGGATATTGCCAAAATATCCGACGCCGAACTCCGTGAGGTGCGCAGAAAAAAGATTGCGATGGTCTTCCAGTCCTTTG  >  NC_000913/2804891‑2805160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑