Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,086,309	G→A	R41R (CGG→CGA)	yqgC →	protein YqgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,086,309	0	G	A	100.0%	215.8 / NA	64	R41R (CGG→CGA)	yqgC	protein YqgC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/28);  total (36/28)

TGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCA  >  NC_000913/3086173‑3086445                                                                                                                                         |                                                                                                                                         tGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGTGTGTGGGGAACGAGTCCTCGCCAGAACGGTGAGACAGGTTAAGAAGCAGACAACGGTTCATTATTTCGTCTCACCTTCACGaccg                                                                                                                                       <  2:395462/140‑1 (MQ=255) tGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGaccg                                                                                                                                       <  1:848512/140‑1 (MQ=255) tGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGaccg                                                                                                                                       >  1:444165/1‑140 (MQ=255)   agaAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGaccgc                                                                                                                                      >  2:394836/1‑139 (MQ=255)   agaAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGaccgc                                                                                                                                      <  1:394836/139‑1 (MQ=255)      aGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGt                                                                                                                                  >  2:856889/1‑140 (MQ=255)        cgcCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTa                                                                                                                                >  1:152133/1‑140 (MQ=255)                tGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAAc                                                                                                                        <  2:152133/140‑1 (MQ=255)                            cGGCAGGTATGCAAAGCAGAGATGCAGAGTGTGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCaaa                                                                                                            >  2:12571/1‑140 (MQ=255)                            cGGCAGGGATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGGTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCaaa                                                                                                            >  2:27818/1‑140 (MQ=255)                             ggCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCaaaa                                                                                                           >  2:1052911/1‑140 (MQ=255)                             ggCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCaaaa                                                                                                           >  1:517620/1‑140 (MQ=255)                             ggCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCaaaa                                                                                                           >  1:160139/1‑140 (MQ=255)                                     tGCAAAGCAGAGATGCAGAGTGCCGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGAtt                                                                                                   >  1:1104408/1‑140 (MQ=255)                                         aaGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCaa                                                                                               <  2:517620/140‑1 (MQ=255)                                          aGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAAc                                                                                              <  2:1170984/140‑1 (MQ=255)                                          aGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCATATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAAc                                                                                              <  1:859983/140‑1 (MQ=255)                                          aGCAGAGATGCAGAGTGCGGGCAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAAc                                                                                              <  2:1153242/140‑1 (MQ=255)                                          aGCAGAGATGCAGAGTGCCGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAAc                                                                                              <  2:1104408/140‑1 (MQ=255)                                             agagATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCg                                                                                           >  1:630622/1‑140 (MQ=255)                                                 aTGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTg                                                                                       >  1:597732/1‑140 (MQ=255)                                                   gCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGa                                                                                     <  1:1052911/140‑1 (MQ=255)                                                      gagTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGt                                                                                  >  1:625793/1‑140 (MQ=255)                                                       agTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTg                                                                                 >  1:443251/1‑140 (MQ=255)                                                         tGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGc                                                                               >  2:1182376/1‑140 (MQ=255)                                                         tGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGc                                                                               >  1:850544/1‑140 (MQ=255)                                                         tGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGc                                                                               >  2:627401/1‑140 (MQ=255)                                                           cGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCaa                                                                             >  1:1025073/1‑140 (MQ=255)                                                           cGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCaa                                                                             >  2:908546/1‑140 (MQ=255)                                                              ggAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAacac                                                                          >  2:494808/1‑140 (MQ=255)                                                                  cGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACgaaa                                                                      <  2:1025073/140‑1 (MQ=255)                                                                        ttCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAAcg                                                                <  1:444376/140‑1 (MQ=255)                                                                         tCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAAcgt                                                               <  2:1091576/140‑1 (MQ=255)                                                                          cACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAAcgtc                                                              >  2:1221145/1‑140 (MQ=255)                                                                          cACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAAcgtc                                                              <  1:908546/140‑1 (MQ=255)                                                                            ccAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAAcgtcgt                                                            <  1:1089636/140‑1 (MQ=255)                                                                               gAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGtgtg                                                         <  1:12571/140‑1 (MQ=255)                                                                                aaCGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGc                                                        >  1:1017399/1‑140 (MQ=255)                                                                                  cGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCgtg                                                           >  1:39492/1‑135 (MQ=255)                                                                                  cGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCgtg                                                           <  2:39492/135‑1 (MQ=255)                                                                                   ggTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCttt                                                     <  2:544871/140‑1 (MQ=255)                                                                                    gTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCtttt                                                    >  1:566658/1‑140 (MQ=255)                                                                                     tGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCttttt                                                   >  2:590578/1‑140 (MQ=255)                                                                                     tGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCtt                                                      >  2:604370/1‑137 (MQ=255)                                                                                     tGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCtt                                                      <  1:604370/137‑1 (MQ=255)                                                                                           aGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTaa                                             <  2:850544/140‑1 (MQ=255)                                                                                                 agcagcAGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCcgcg                                       >  1:674171/1‑140 (MQ=255)                                                                                                  gcagcaGACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCcgcgc                                      >  1:799179/1‑140 (MQ=255)                                                                                                     gcagACAACGGTTCATTATTTCGTATCGCCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAATCTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGcgcc                                   <  2:1022537/140‑1 (MQ=255)                                                                                                       agACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGcgccgc                                 >  2:1100750/1‑140 (MQ=255)                                                                                                       agACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGcgccgc                                 >  1:414898/1‑140 (MQ=255)                                                                                                        gACAACGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCcgcg                                <  2:160139/140‑1 (MQ=255)                                                                                                             cGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTta                           <  1:1100750/140‑1 (MQ=255)                                                                                                             cGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTta                           <  1:627401/140‑1 (MQ=255)                                                                                                             cGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTta                           <  2:799179/140‑1 (MQ=255)                                                                                                             cGGTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTta                           <  2:760734/140‑1 (MQ=255)                                                                                                              ggTTCATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTtat                          >  2:692064/1‑140 (MQ=255)                                                                                                                  cATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGcgcc                                   >  2:305905/1‑127 (MQ=255)                                                                                                                  cATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGcgcc                                   <  1:305905/127‑1 (MQ=255)                                                                                                                  cATTATTTCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATAccc                      >  1:191257/1‑140 (MQ=255)                                                                                                                       tttCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACaa                 <  2:597732/140‑1 (MQ=255)                                                                                                                       tttCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACaa                 <  2:1115463/140‑1 (MQ=255)                                                                                                                       tttCGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACaa                 >  2:709869/1‑140 (MQ=255)                                                                                                                          cGTATCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATgg              >  1:8663/1‑140 (MQ=255)                                                                                                                              tCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACaa                 <  2:13502/133‑1 (MQ=255)                                                                                                                              tCACCTCCACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACaa                 >  1:13502/1‑133 (MQ=255)                                                                                                                                    ccACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATAccc                      <  1:45842/122‑1 (MQ=255)                                                                                                                                    ccACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATAccc                      >  2:45842/1‑122 (MQ=255)                                                                                                                                    ccACGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTg    >  1:1015833/1‑140 (MQ=255)                                                                                                                                      aCGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCa  <  2:630622/140‑1 (MQ=255)                                                                                                                                      aCGACCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCa  <  1:494808/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGAGATGCAGAGTGCGGGGAACGAATCTTCACCAGAACGGTGAGACAGGTTAAGCAGCAGACAACGGTTCATTATTTCGTATCACCTCCACGGCCGCCTGTTAAGACGAACCCACAAGCCAAAACTCTGATTTCAACCCGGCTGGAAGTGGCAACACGAAAGAAACGTCGTGTGCTTTTTATTTAAGCCGCGCGCCGCGTTTTATACCCCACAATGGCAGAAAATTGCA  >  NC_000913/3086173‑3086445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑