Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,466,452	A→G	G265G (GGT→GGC)	treR ←	DNA‑binding transcriptional repressor TreR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,466,452	0	A	G	100.0%	294.7 / NA	78	G265G (GGT→GGC)	treR	DNA‑binding transcriptional repressor TreR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/40);  total (38/40)

ATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCT  >  NC_000913/4466318‑4466587                                                                                                                                       |                                                                                                                                        atgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGAc                                                                                                                                    >  2:1091680/1‑140 (MQ=255)  tgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACg                                                                                                                                   <  1:567361/140‑1 (MQ=255)         ttgttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCa                                                                                                                            <  2:498924/140‑1 (MQ=255)             tgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACTCTCGCCAGTTg                                                                                                                        <  2:425132/140‑1 (MQ=255)             tgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTg                                                                                                                        >  2:343624/1‑140 (MQ=255)                     gCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGgtgt                                                                                                                <  1:1091680/140‑1 (MQ=255)                       tGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCa                                                                                                                      <  1:110884/132‑1 (MQ=255)                       tGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCa                                                                                                                      >  2:110884/1‑132 (MQ=255)                           cccGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGc                                                                                                          >  2:1269151/1‑140 (MQ=255)                            ccGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCg                                                                                                         <  1:293899/140‑1 (MQ=255)                              ggTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGtt                                                                                                       >  2:525287/1‑140 (MQ=255)                              ggTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGtt                                                                                                       <  1:99736/140‑1 (MQ=255)                                 tACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGct                                                                                                    <  1:659069/140‑1 (MQ=255)                                   ccTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGctct                                                                                                  >  2:104039/1‑140 (MQ=255)                                      gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTg                                                                                                <  1:1002850/139‑1 (MQ=255)                                      gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTg                                                                                                >  2:1002850/1‑139 (MQ=255)                                      gcgcGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGc                                                                                               <  1:171177/140‑1 (MQ=255)                                        gcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAg                                                                                             >  2:417644/1‑140 (MQ=255)                                            tCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                                                         >  2:417943/1‑140 (MQ=255)                                             cAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAtt                                                                                        <  2:70731/140‑1 (MQ=255)                                               aCTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTa                                                                                      <  1:824608/140‑1 (MQ=255)                                                 tGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACt                                                                                    <  1:525287/140‑1 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGc                                                                                               >  1:1219446/1‑124 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGc                                                                                               <  2:1219446/124‑1 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcgc                                                                               >  2:102757/1‑140 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcgc                                                                               >  2:335318/1‑140 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcgc                                                                               >  2:1164209/1‑140 (MQ=255)                                                      aGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgcgc                                                                               >  2:284785/1‑140 (MQ=255)                                                       gccgccTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGcc                                                                              <  1:436024/140‑1 (MQ=255)                                                           ccTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                                                         <  2:795818/125‑1 (MQ=255)                                                           ccTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAt                                                                                         >  1:795818/1‑125 (MQ=255)                                                             tGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGc                                                                        <  1:191550/140‑1 (MQ=255)                                                              ggCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGcc                                                                       <  1:523663/140‑1 (MQ=255)                                                                  tCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCg                                                                   >  2:207988/1‑140 (MQ=255)                                                                   ccAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCgt                                                                  >  2:536974/1‑140 (MQ=255)                                                                          cGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGtt                                                           <  1:343624/140‑1 (MQ=255)                                                                            gCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgc                                                         <  2:1235529/140‑1 (MQ=255)                                                                                aaCCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGcac                                                     <  1:1164209/140‑1 (MQ=255)                                                                                 aCCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGcaca                                                    >  1:665697/1‑140 (MQ=255)                                                                                   cGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGt                                                  <  1:284785/140‑1 (MQ=255)                                                                                     ggATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTaa                                                >  2:518747/1‑140 (MQ=255)                                                                                         cTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCg                                            <  1:169534/140‑1 (MQ=255)                                                                                           aCGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGt                                          >  1:215921/1‑140 (MQ=255)                                                                                           aCGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGt                                          <  1:1179085/140‑1 (MQ=255)                                                                                            cGGTTACGATCTCCGGATGGGGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa                                         >  2:825222/1‑140 (MQ=255)                                                                                            cGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa                                         >  1:1155824/1‑140 (MQ=255)                                                                                               ttACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCg                                            <  1:339984/134‑1 (MQ=255)                                                                                               ttACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCg                                            >  2:339984/1‑134 (MQ=255)                                                                                               ttACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGtt                                      >  2:227774/1‑140 (MQ=255)                                                                                               ttACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGtt                                      >  2:48038/1‑140 (MQ=255)                                                                                                 aCGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTc                                    >  2:319431/1‑140 (MQ=255)                                                                                                  cGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt                                     >  2:1209435/1‑138 (MQ=255)                                                                                                  cGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGttt                                     <  1:1209435/138‑1 (MQ=255)                                                                                                  cGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCACACAGTAAGGCgg                                           <  2:1132550/132‑1 (MQ=255)                                                                                                  cGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCACACAGTAAGGCgg                                           >  1:1132550/1‑132 (MQ=255)                                                                                                   gATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAg                                                   >  1:1032255/1‑123 (MQ=255)                                                                                                   gATCTCCGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAg                                                   <  2:1032255/123‑1 (MQ=255)                                                                                                         cGGATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTaa                            >  1:290971/1‑140 (MQ=255)                                                                                                           gATGGAGGAATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATc                          <  2:848971/140‑1 (MQ=255)                                                                                                                aggaATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACttt                     >  2:1150415/1‑140 (MQ=255)                                                                                                                aggaATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACttt                     >  1:865230/1‑140 (MQ=255)                                                                                                                aggaATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACttt                     >  1:104994/1‑140 (MQ=255)                                                                                                                aggaATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACttt                     >  2:575517/1‑140 (MQ=255)                                                                                                                 ggaATTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACtttt                    >  1:622855/1‑140 (MQ=255)                                                                                                                   aaTTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGc                  <  2:1155824/140‑1 (MQ=255)                                                                                                                   aaTTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGc                  <  2:622855/140‑1 (MQ=255)                                                                                                                   aaTTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGc                  <  1:48038/140‑1 (MQ=255)                                                                                                                   aaTTTCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGc                  <  1:227774/140‑1 (MQ=255)                                                                                                                      ttCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAAc               <  1:319431/140‑1 (MQ=255)                                                                                                                       tCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACg              <  2:1260860/140‑1 (MQ=255)                                                                                                                       tCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACg              <  1:1269151/140‑1 (MQ=255)                                                                                                                       tCATTAACGGCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACg              <  1:575517/140‑1 (MQ=255)                                                                                                                               ggCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCa        <  1:408962/138‑1 (MQ=255)                                                                                                                               ggCGTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCa        >  2:408962/1‑138 (MQ=255)                                                                                                                                  gTATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGcc   <  2:665697/140‑1 (MQ=255)                                                                                                                                   tATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCt  >  1:1268679/1‑140 (MQ=255)                                                                                                                                   tATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCt  >  1:1257570/1‑140 (MQ=255)                                                                                                                                   tATTGCCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCt  <  2:290971/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCT  >  NC_000913/4466318‑4466587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑