Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,088	(C)8→7	intergenic (‑85/+172)	iraM ← / ← ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,081	0	C	.	100.0%	168.2 / NA	40	intergenic (‑78/+179)	iraM/ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (29/11);  total (29/11)

GCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211945‑1212216                                                                                                                                         |                                                                                                                                        gCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAcccc                                                                                                                                      >  1:576448/1‑140 (MQ=255)  cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:315128/1‑140 (MQ=255)  cTGAAAAAGATATACCACATGTAGGTTGAATTACCGAGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:394479/1‑140 (MQ=255)                ccACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAaaataa                                                                                                                      <  2:762319/140‑1 (MQ=255)                    aTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaatta                                                                                                                  >  1:432912/1‑140 (MQ=255)                        aGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAattaatta                                                                                                              <  1:73601/140‑1 (MQ=255)                        aGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAattaatta                                                                                                              >  2:291905/1‑140 (MQ=255)                        aGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAattaatta                                                                                                              >  1:548811/1‑140 (MQ=255)                          gTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTaaa                                                                                                            >  1:121674/1‑140 (MQ=255)                               aTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatgat                                                                                                       <  2:779477/140‑1 (MQ=255)                                        tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTtatat                                                                                              >  1:121089/1‑140 (MQ=255)                                         cAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTtatata                                                                                             <  2:432912/140‑1 (MQ=255)                                                tATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaaataaa                                                                                      >  1:107732/1‑140 (MQ=255)                                                tATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaaataaa                                                                                      >  1:486627/1‑140 (MQ=255)                                                 aTCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGCATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATAtaaaataaaa                                                                                     >  2:704333/1‑140 (MQ=255)                                                  tCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATaaaataaaat                                                                                    >  2:622135/1‑140 (MQ=255)                                                      cTTCCTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                <  2:739919/140‑1 (MQ=255)                                                      cTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                <  1:236412/140‑1 (MQ=255)                                                          aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                            >  1:561970/1‑140 (MQ=255)                                                          aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                            <  2:344170/140‑1 (MQ=255)                                                              gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGa                                                                        <  2:107732/140‑1 (MQ=255)                                                              gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGa                                                                        >  1:676582/1‑140 (MQ=255)                                                                 aTGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAtc                                                                     >  2:255965/1‑140 (MQ=255)                                                                      ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                >  2:694463/1‑140 (MQ=255)                                                                         tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                             >  2:252900/1‑140 (MQ=255)                                                                              cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTa                                                        >  2:188870/1‑140 (MQ=255)                                                                              cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTa                                                        >  1:327596/1‑140 (MQ=255)                                                                                           ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  2:527839/1‑140 (MQ=255)                                                                                           ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  1:788843/1‑140 (MQ=255)                                                                                           ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  2:637985/1‑140 (MQ=255)                                                                                             ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  1:66625/1‑140 (MQ=255)                                                                                             ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  2:514368/1‑140 (MQ=255)                                                                                             ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  1:613338/1‑140 (MQ=255)                                                                                             ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  1:548311/1‑140 (MQ=255)                                                                                                 ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtgtg                                     <  2:486627/140‑1 (MQ=255)                                                                                                       tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               <  1:718515/140‑1 (MQ=255)                                                                                                           tATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATaa                           >  2:641070/1‑140 (MQ=255)                                                                                                                   gATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGa                   >  2:551217/1‑140 (MQ=255)                                                                                                                   gATTTTGCATATGAGTATATTA‑CCCCCCCTAAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGa                   >  1:452059/1‑140 (MQ=255)                                                                                                                     ttttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACt                 <  2:788843/140‑1 (MQ=255)                                                                                                                               gAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGa       >  2:367089/1‑140 (MQ=255)                                                                                                                                    tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  >  2:67510/1‑140 (MQ=255)                                                                                                                                    tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  >  1:212027/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211945‑1212216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑