Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,838,177	Δ1 bp	coding (966/1536 nt)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,838,174	0	G	.	100.0%	181.7 / NA	43	coding (963/1536 nt)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/23);  total (20/23)

CCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGC  >  NC_000913/1838037‑1838297                                                                                                                                          |                                                                                                                            ccTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATggg                                                                                                                           >  2:709032/1‑140 (MQ=255) ccTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATggg                                                                                                                           >  1:473635/1‑140 (MQ=255) ccTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATggg                                                                                                                           >  2:358603/1‑140 (MQ=255) ccTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATggg                                                                                                                           <  2:533081/140‑1 (MQ=255)     ccACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATgggctgg                                                                                                                       >  2:355263/1‑136 (MQ=255)                     gggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGc                                                                                                      >  1:643006/1‑140 (MQ=255)                     gggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGc                                                                                                      >  1:414455/1‑140 (MQ=255)                      ggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCt                                                                                                     <  1:712420/140‑1 (MQ=255)                         aaTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTg                                                                                                  <  1:709032/140‑1 (MQ=255)                                       ttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgct                                                                                    <  2:56059/140‑1 (MQ=255)                                         ttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCtgt                                                                                  <  2:587667/140‑1 (MQ=255)                                                  tAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAAt                                                                         >  1:629067/1‑140 (MQ=255)                                                   aaCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgct                                                                                    <  2:655622/128‑1 (MQ=255)                                                   aaCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgct                                                                                    >  1:655622/1‑128 (MQ=255)                                                        gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGc                                                                   >  2:137963/1‑140 (MQ=255)                                                         tgtgCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATg                                                                        <  2:495398/134‑1 (MQ=255)                                                         tgtgCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATg                                                                        >  1:495398/1‑134 (MQ=255)                                                           tgCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcac                                                                >  2:466386/1‑140 (MQ=255)                                                              tgtgtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGc                                                             <  1:107896/140‑1 (MQ=255)                                                                tgtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGt                                                           >  1:788134/1‑140 (MQ=255)                                                                  tgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCg                                                         >  1:174800/1‑140 (MQ=255)                                                                   gtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGc                                                        <  1:137963/140‑1 (MQ=255)                                                                   gtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGc                                                        <  1:358603/140‑1 (MQ=255)                                                                    tgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGcc                                                       <  1:466386/140‑1 (MQ=255)                                                                       ttCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGt                                                    >  2:19448/1‑140 (MQ=255)                                                                       ttCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGt                                                    >  1:269199/1‑140 (MQ=255)                                                                        tCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGtt                                                   <  2:552996/140‑1 (MQ=255)                                                                          tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtgt                                                 >  2:625519/1‑140 (MQ=255)                                                                          tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtgt                                                 >  2:601745/1‑140 (MQ=255)                                                                            ctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTgg                                               >  1:392295/1‑140 (MQ=255)                                                                                     cTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTg                                      >  2:353272/1‑140 (MQ=255)                                                                                       cgcgGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGcc                                    <  2:269199/140‑1 (MQ=255)                                                                                       cgcgGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGcc                                    <  2:414455/140‑1 (MQ=255)                                                                                        gcgGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGccc                                   <  2:174800/140‑1 (MQ=255)                                                                                        gcgGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGccc                                   <  1:19448/140‑1 (MQ=255)                                                                                                   gacgaTCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAcc                        <  2:629067/140‑1 (MQ=255)                                                                                                        tCAATAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctc                   <  2:473635/140‑1 (MQ=255)                                                                                                           aTAGTGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGc                >  1:165797/1‑140 (MQ=255)                                                                                                               tGAAGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACt            <  2:252317/140‑1 (MQ=255)                                                                                                                  aGCGCTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggt         >  1:511683/1‑140 (MQ=255)                                                                                                                    cgcTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtgg       >  1:140017/1‑140 (MQ=255)                                                                                                                    cgcTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtgg       <  2:165797/140‑1 (MQ=255)                                                                                                                    cgcTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtgg       <  1:625519/140‑1 (MQ=255)                                                                                                                      cTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCg     >  2:548800/1‑140 (MQ=255)                                                                                                                      cTCATCAACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCg     >  2:569786/1‑140 (MQ=255)                                                                                                                        catcaACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCggg   <  2:140017/140‑1 (MQ=255)                                                                                                                         atcaACAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc  <  1:548800/140‑1 (MQ=255)                                                                                                                              cAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc  >  1:428349/1‑135 (MQ=255)                                                                                                                              cAGCCTGACAAT‑GGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc  <  2:428349/135‑1 (MQ=255)                                                                                                                                          |                                                                                                                            CCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGC  >  NC_000913/1838037‑1838297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑