Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,203	(T)7→8	coding (293/357 nt)	yhaI →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,196	1	.	T	100.0%	109.3 / NA	32	V96F (GTT→TTT)	yhaI	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (13/19);  total (13/19)

TCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑TTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTA  >  NC_000913/3253060‑3253320                                                                                                                                          |                                                                                                                             tCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑ttt                                                                                                                           >  2:429652/1‑140 (MQ=255)     tCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑ttttttt                                                                                                                       >  2:352151/1‑140 (MQ=255)          gCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑ttttttttctgc                                                                                                                  <  2:665087/140‑6 (MQ=255)           cTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGca                                                                                                                  <  1:429652/140‑1 (MQ=255)               aTTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGa                                                                                                              >  1:161132/1‑140 (MQ=255)                tttGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCTGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGaa                                                                                                             <  1:72332/140‑1 (MQ=255)                 ttGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAg                                                                                                            <  2:161132/140‑1 (MQ=255)                   gCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGC‑TACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTa                                                                                                         <  1:569103/140‑1 (MQ=255)                    cTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTa                                                                                                         >  2:486213/1‑140 (MQ=255)                     tGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTAc                                                                                                        >  1:613911/1‑140 (MQ=255)                     tGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑tttttttt                                                                                                                      >  1:320812/1‑124 (MQ=255)                     tGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑tttttttt                                                                                                                      <  2:320812/125‑2 (MQ=255)                        cAACCTTCCTTCCCGTCCTTTCGCCTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAg                                                                                                            >  2:80758/1‑133 (MQ=255)                        cAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAg                                                                                                            <  1:80758/133‑1 (MQ=255)                          aCCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTg                                                                                                   >  1:690459/1‑140 (MQ=255)                              tccttccCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGgcag                                                                                               <  1:305592/140‑1 (MQ=255)                               ccttccCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGgcagc                                                                                              <  2:437516/140‑1 (MQ=255)                                cttccCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGgcagca                                                                                             <  1:80817/140‑1 (MQ=255)                                  tccCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAAt                                                                                           <  2:211980/140‑1 (MQ=255)                                  tccCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAAt                                                                                           <  2:690459/140‑1 (MQ=255)                                      gTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGtt                                                                                       >  2:765143/1‑140 (MQ=255)                                       tCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTa                                                                                      >  2:254040/1‑140 (MQ=255)                                             cgcTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGaaa                                                                                <  1:492972/140‑1 (MQ=255)                                                        cGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGt                                                                     <  1:254040/140‑1 (MQ=255)                                                        cGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGt                                                                     <  1:352151/140‑1 (MQ=255)                                                             cTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGt                                                                <  1:765143/140‑1 (MQ=255)                                                                              gTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGaa                                               >  1:653073/1‑140 (MQ=255)                                                                                                tGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGcc                             >  2:728666/1‑140 (MQ=255)                                                                                                tGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGc                              <  2:36019/139‑1 (MQ=255)                                                                                                tGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGc                              >  1:36019/1‑139 (MQ=255)                                                                                                 gCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGccc                            >  2:485642/1‑140 (MQ=255)                                                                                                     ttttttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCttt                        <  1:728666/140‑1 (MQ=255)                                                                                                       ttttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCttttt                      >  1:384524/1‑140 (MQ=255)                                                                                                         ttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttat                    <  1:628780/140‑1 (MQ=255)                                                                                                              ccGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACa               <  2:653073/140‑1 (MQ=255)                                                                                                                 tttATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTaa                 >  2:462336/1‑135 (MQ=255)                                                                                                                 tttATTGGCTGGCTCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTaa                 <  1:462336/135‑1 (MQ=255)                                                                                                                           ggctCGTGCTTCTGGTTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAAtatta  >  2:533894/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                             TCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGG‑TTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTA  >  NC_000913/3253060‑3253320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑