Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,376	(A)6→7	noncoding (194/216 nt)	sraG →	small regulatory RNA SraG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,370	1	.	A	100.0%	130.5 / NA	38	noncoding (188/216 nt)	sraG	small regulatory RNA SraG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (23/15);  total (23/15)

ATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTG‑AAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAG  >  NC_000913/3311236‑3311503                                                                                                                                        |                                                                                                                                      aTCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTG‑aaaaa                                                                                                                                  >  2:193130/1‑140 (MQ=255)  tCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTG‑aaaaaa                                                                                                                                 <  2:743114/140‑1 (MQ=255)                    aCCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGcccc                                                                                                                <  2:335315/140‑1 (MQ=255)                     cccTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGccccc                                                                                                               >  2:283793/1‑140 (MQ=255)                               gCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGc                                                                                                                   <  2:433987/126‑1 (MQ=255)                               gCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGc                                                                                                                   >  1:433987/1‑126 (MQ=255)                               gCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGaaa                                                                                                     >  1:713869/1‑140 (MQ=255)                                ctctATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGccccc                                                                                                               >  2:309722/1‑129 (MQ=255)                                ctctATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGccccc                                                                                                               <  1:309722/129‑1 (MQ=255)                                ctctATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAAc                                                                                                    >  2:403068/1‑140 (MQ=255)                                ctctATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAAc                                                                                                    >  1:335702/1‑140 (MQ=255)                                ctctATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAAc                                                                                                    >  1:680875/1‑140 (MQ=255)                                         ggTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGaa                                                                                           >  2:654494/1‑140 (MQ=255)                                             aaGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAg                                                                                       <  1:372483/140‑1 (MQ=255)                                             aaGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAg                                                                                       >  1:339789/1‑140 (MQ=255)                                                 ctctCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGAc                                                                                   <  2:483302/140‑1 (MQ=255)                                                        tAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGAcc                                                                            <  1:433038/140‑1 (MQ=255)                                                           ccGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAg                                                                         >  2:526167/1‑140 (MQ=255)                                                              cgcgAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGgcg                                                                      >  1:363553/1‑140 (MQ=255)                                                                cgAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCt                                                                    >  2:627445/1‑140 (MQ=255)                                                                         gCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCt                                                           <  1:283793/140‑1 (MQ=255)                                                                              ggAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGgtg                                                      <  2:680875/140‑1 (MQ=255)                                                                                aaGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTa                                                    <  1:306107/140‑1 (MQ=255)                                                                                              caacaaTACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAATGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCtt                                      <  2:568424/140‑1 (MQ=255)                                                                                              caacaaTACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCtt                                      <  1:403068/140‑1 (MQ=255)                                                                                              caacaaTACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCtt                                      >  1:79270/1‑140 (MQ=255)                                                                                              caacaaTACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCtt                                      >  2:744008/1‑140 (MQ=255)                                                                                               aacaaTACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCttt                                     >  1:87847/1‑140 (MQ=255)                                                                                                          aGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGt                          >  2:680623/1‑140 (MQ=255)                                                                                                           gTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTa                         >  2:279136/1‑140 (MQ=255)                                                                                                           gTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTa                         >  2:166911/1‑140 (MQ=255)                                                                                                              tCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTc                      >  2:349289/1‑140 (MQ=255)                                                                                                              tCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTc                      >  1:652275/1‑140 (MQ=255)                                                                                                                 aGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAg                   >  1:116845/1‑140 (MQ=255)                                                                                                                 aGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAg                   <  1:682485/140‑1 (MQ=255)                                                                                                                 aGTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAg                   >  1:560542/1‑140 (MQ=255)                                                                                                                  gTGAATTGCTGCCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGc                  <  1:744008/140‑1 (MQ=255)                                                                                                                        tgctgcCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTa            <  1:279136/140‑1 (MQ=255)                                                                                                                           tgcCGTCAGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTacga         <  1:662149/140‑1 (MQ=255)                                                                                                                                  aGCTTGAAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTgag  >  2:684192/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      ATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTG‑AAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCCAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAG  >  NC_000913/3311236‑3311503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑