Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,613	(G)5→6	intergenic (‑563/‑112)	yhcC ← / → gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,608	1	.	G	100.0%	295.1 / NA	83	intergenic (‑558/‑117)	yhcC/gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (44/39);  total (44/39)

TTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAA‑GGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATA  >  NC_000913/3354475‑3354737                                                                                                                                       |                                                                                                                                  tttGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAA‑gggggg                                                                                                                             >  2:659686/1‑139 (MQ=255)             taGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGgc                                                                                                                  <  2:133524/139‑1 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTACTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCgggcg                                                                                                                  <  1:702486/133‑5 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCgggcg                                                                                                                  >  2:702486/1‑129 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAgg                                                                                                                   <  2:217721/132‑1 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAgg                                                                                                                   >  1:217721/1‑132 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGgcg                                                                                                                 >  1:530929/1‑134 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGgcg                                                                                                                 <  2:530929/134‑1 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:95061/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:326716/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:275868/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:633451/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  1:735664/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:237677/1‑140 (MQ=255)                    cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTa                                                                                                           >  2:123733/1‑140 (MQ=255)                     ccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTAt                                                                                                          <  2:720265/140‑1 (MQ=255)                      cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                         <  1:261402/140‑1 (MQ=255)                       gATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGa                                                                                                        <  1:624090/140‑1 (MQ=255)                       gATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGa                                                                                                        <  1:326716/140‑1 (MQ=255)                               ggAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa                                                                                                >  1:423045/1‑140 (MQ=255)                                       gCTGGAAGCTTTCTGGATGAGCAGCCTGCTTATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCg                                                                                        >  1:206374/1‑140 (MQ=255)                                       gCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCg                                                                                        >  2:607142/1‑140 (MQ=255)                                        cTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                                                       <  2:423045/140‑1 (MQ=255)                                          ggAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa                                                                                     <  1:39781/140‑1 (MQ=255)                                          ggAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa                                                                                     <  1:237677/140‑1 (MQ=255)                                          ggAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCACGTCGCCa                                                                                     <  2:558752/140‑1 (MQ=255)                                              gCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct                                                                                 <  1:633451/140‑1 (MQ=255)                                                      gATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                                                         >  2:282508/1‑140 (MQ=255)                                                        tGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGc                                                                       <  2:735664/140‑1 (MQ=255)                                                          agcagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                                                     >  1:414043/1‑140 (MQ=255)                                                            cagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                                                                   >  2:761289/1‑140 (MQ=255)                                                                      atcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt                                                         >  2:460271/1‑140 (MQ=255)                                                                         atatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg                                                      >  2:442614/1‑140 (MQ=255)                                                                             ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                                                             >  2:31119/1‑129 (MQ=255)                                                                             ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                                                             <  1:31119/129‑1 (MQ=255)                                                                             ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                                                  >  2:230944/1‑140 (MQ=255)                                                                             ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                                                  >  1:671163/1‑140 (MQ=255)                                                                              tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGt                                                 <  2:206374/140‑1 (MQ=255)                                                                                tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                                               >  1:483268/1‑140 (MQ=255)                                                                                tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                                               >  1:184571/1‑140 (MQ=255)                                                                                     tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc                                          >  1:730312/1‑140 (MQ=255)                                                                                     tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc                                          >  1:438485/1‑140 (MQ=255)                                                                                       aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTccc                                        >  2:469300/1‑140 (MQ=255)                                                                                       aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTccc                                        >  2:122397/1‑140 (MQ=255)                                                                                          gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  1:659686/140‑1 (MQ=255)                                                                                          gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  1:607142/140‑1 (MQ=255)                                                                                          gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  2:414043/140‑1 (MQ=255)                                                                                          gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  2:438485/140‑1 (MQ=255)                                                                                           agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCag                                    <  1:129185/140‑1 (MQ=255)                                                                                             aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCagag                                  >  1:85463/1‑140 (MQ=255)                                                                                             aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCagag                                  >  1:465129/1‑140 (MQ=255)                                                                                               ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGcc                                >  1:679887/1‑140 (MQ=255)                                                                                               ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGcc                                >  1:364103/1‑140 (MQ=255)                                                                                               ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGcc                                >  2:527294/1‑140 (MQ=255)                                                                                                 ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt                                           <  2:233253/127‑1 (MQ=255)                                                                                                 ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt                                           >  1:233253/1‑127 (MQ=255)                                                                                                  ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTgg                             >  1:137301/1‑140 (MQ=255)                                                                                                     ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTggggg                          <  1:230944/140‑1 (MQ=255)                                                                                                       cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     >  1:324601/1‑127 (MQ=255)                                                                                                       cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  2:324601/127‑1 (MQ=255)                                                                                                         ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGt                      <  1:761289/140‑1 (MQ=255)                                                                                                         ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGt                      <  1:460271/140‑1 (MQ=255)                                                                                                          cGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGtt                     >  2:293597/1‑140 (MQ=255)                                                                                                          cGTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGtt                     <  1:282508/140‑1 (MQ=255)                                                                                                           gTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTggggg                          <  1:280556/134‑1 (MQ=255)                                                                                                           gTATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTggggg                          >  2:280556/1‑134 (MQ=255)                                                                                                            tATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTTCCGCAGAGCCTGGGGGAGGTTCa                   <  1:363666/140‑1 (MQ=255)                                                                                                            tATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     >  1:428910/1‑122 (MQ=255)                                                                                                            tATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa                                     <  2:428910/122‑1 (MQ=255)                                                                                                            tATTAACCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCa                   >  1:302439/1‑140 (MQ=255)                                                                                                                aaCCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGat               <  1:95061/140‑1 (MQ=255)                                                                                                                aaCCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGat               <  1:275868/140‑1 (MQ=255)                                                                                                                 aCCGATGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGata              <  1:293597/140‑1 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTc                    <  1:276292/129‑1 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTc                    >  2:276292/1‑129 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGata              >  1:281454/1‑135 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGata              <  2:281454/135‑1 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAtgttg         >  1:37781/1‑140 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAtgttg         <  2:37781/140‑1 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAtgttg         >  2:364414/1‑140 (MQ=255)                                                                                                                      tGCGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAtgttg         >  2:231524/1‑140 (MQ=255)                                                                                                                        cGAAAAGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTa       <  2:137301/140‑1 (MQ=255)                                                                                                                           aaaGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGa    <  1:469300/140‑1 (MQ=255)                                                                                                                             aGGACAACAAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATa  <  2:465129/140‑1 (MQ=255)                                                                                                                                  aacaagGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGata              >  1:25150/1‑123 (MQ=255)                                                                                                                                  aacaagGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGata              <  2:25150/123‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAA‑GGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATA  >  NC_000913/3354475‑3354737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑