| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,379,266 | (C)7→8 | coding (732/1128 nt) | zapE ← | cell division factor ZapE with ATPase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,379,259 | 1 | . | C | 96.3% | 182.6 / ‑0.2 | 54 | coding (739/1128 nt) | zapE | cell division factor ZapE with ATPase activity |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (29/23); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTA > NC_000913/3379128‑3379387 | cgcAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑cccccccc > 1:327769/1‑139 (MQ=255) cAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑ccccccccgc < 2:327769/140‑4 (MQ=255) taGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAACGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCATT‑TC‑CCCCCCCGCCAgcg < 1:619631/140‑1 (MQ=255) tCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCa > 1:182106/1‑140 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAgcg > 2:452142/1‑134 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAgcg < 1:452142/134‑1 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAAcc < 1:636655/140‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCAt > 2:800192/1‑140 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCAt > 2:145419/1‑140 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCAt > 1:443420/1‑140 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCAt > 1:654386/1‑140 (MQ=255) gACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAg < 2:141564/140‑1 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGt > 2:32429/1‑140 (MQ=255) aCTGCGGGCGTCGACACACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGt > 2:275924/1‑140 (MQ=255) tGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGttt > 1:25735/1‑140 (MQ=255) tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCAttt > 2:728517/1‑140 (MQ=255) tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCAttt > 1:593929/1‑140 (MQ=255) cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc < 2:443420/140‑1 (MQ=255) acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg > 2:771797/1‑140 (MQ=255) aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg < 1:272742/140‑1 (MQ=255) gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa > 1:672012/1‑140 (MQ=255) gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa > 1:350080/1‑140 (MQ=255) gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt > 1:92714/1‑140 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg > 2:487979/1‑140 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg > 1:604395/1‑140 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg > 2:216747/1‑140 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc < 2:92714/140‑1 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCCCCAGCGCCAACCATAGTTTATCCTTTTGCGCCCGGGTTTCTTCGTGAAGGGGc > 2:514553/1‑140 (MQ=255) gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg < 1:275924/140‑1 (MQ=255) cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa < 1:422641/140‑1 (MQ=255) cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa < 2:433218/129‑1 (MQ=255) cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa > 1:433218/1‑129 (MQ=255) cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTCC‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc < 2:672012/140‑1 (MQ=255) cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc < 1:800192/140‑1 (MQ=255) aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCt < 2:604395/140‑1 (MQ=255) gCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga > 1:273758/1‑140 (MQ=255) aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga < 1:240529/140‑1 (MQ=255) cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg < 1:728517/140‑1 (MQ=255) tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGGGTACg > 1:471072/1‑140 (MQ=255) tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc > 2:465052/1‑140 (MQ=255) ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGAc > 2:402414/1‑140 (MQ=255) gCCGATGGTTTATTTTTAACGTCGGGTAATTTTCCCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACg < 1:514553/140‑1 (MQ=255) ccGATGGTTGTTTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa < 2:164930/140‑1 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTCC‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa < 2:779237/140‑1 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt > 2:270893/1‑128 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt < 1:270893/128‑1 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa < 1:402414/140‑1 (MQ=255) cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa < 1:393431/140‑1 (MQ=255) gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATa > 1:369314/1‑140 (MQ=255) aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATaa > 1:565595/1‑140 (MQ=255) tGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAAt < 2:654386/140‑1 (MQ=255) tttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACAcc > 2:159213/1‑140 (MQ=255) ttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCa < 2:353530/140‑1 (MQ=255) cGTCGGTGATTTTTCTCGTTCC‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 2:729179/140‑1 (MQ=255) cGTCGGTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 1:159213/140‑1 (MQ=255) ggTGAATTTTCTCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGt > 2:64708/1‑140 (MQ=255) ttttCCCGTT‑TC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTa < 2:593929/140‑1 (MQ=255) | CGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTA > NC_000913/3379128‑3379387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |