| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,093,358 | (G)6→7 | coding (483/1359 nt) | dgcT → | putative diguanylate cyclase DgcT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,093,352 | 1 | . | G | 97.5% | 135.3 / ‑3.3 | 40 | coding (477/1359 nt) | dgcT | putative diguanylate cyclase DgcT |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (19/20); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGT > NC_000913/1093213‑1093485 | cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTtgttttttgttttt > 1:706875/1‑140 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑ggg > 2:196596/1‑140 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑ggg < 2:15111/140‑1 (MQ=255) cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑gggg < 2:520161/140‑1 (MQ=255) tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑gggggggc < 1:460430/140‑3 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑gggggggccg > 2:547625/1‑136 (MQ=255) gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATa > 1:466241/1‑140 (MQ=255) ttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGt > 2:85658/1‑140 (MQ=255) ttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGt > 1:753916/1‑140 (MQ=255) cGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAGCAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTg > 2:706666/1‑140 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTc < 1:196596/140‑1 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTc > 1:504755/1‑140 (MQ=255) tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTa < 1:569175/140‑1 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATgaga > 1:202658/1‑140 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGTCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCt > 1:127817/1‑140 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCt < 2:644157/140‑1 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCt > 2:156799/1‑140 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTa < 1:706666/140‑1 (MQ=255) ggCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTAc < 1:373280/140‑1 (MQ=255) tGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTa < 1:314948/134‑1 (MQ=255) tGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTa > 2:314948/1‑134 (MQ=255) ggTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTa > 1:176187/1‑140 (MQ=255) ggTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTac > 2:155140/1‑140 (MQ=255) tGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTaca < 2:800623/140‑1 (MQ=255) tGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTaca < 2:486999/140‑1 (MQ=255) ttAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGa > 1:21734/1‑140 (MQ=255) ttAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGa > 1:286682/1‑140 (MQ=255) gTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGtt > 1:6726/1‑140 (MQ=255) aaaaCACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAAc < 2:21734/140‑1 (MQ=255) aaCACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTg > 2:184820/1‑128 (MQ=255) aaCACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTg < 1:184820/128‑1 (MQ=255) acacAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCaa < 2:504755/140‑1 (MQ=255) aaaaCAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTg < 2:175937/123‑1 (MQ=255) aaaaCAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTg > 1:175937/1‑123 (MQ=255) gcAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCaa < 2:275933/128‑1 (MQ=255) gcAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCaa > 1:275933/1‑128 (MQ=255) cAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAg < 1:430318/140‑1 (MQ=255) tttCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCa < 1:389124/131‑1 (MQ=255) tttCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCa > 2:389124/1‑131 (MQ=255) ccAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCa < 2:470871/128‑1 (MQ=255) ccAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCa > 1:470871/1‑128 (MQ=255) ccAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGaa > 2:677761/1‑140 (MQ=255) ccAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGaa < 1:547625/140‑1 (MQ=255) aaaTGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTc < 2:342958/140‑1 (MQ=255) aaTGACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCa < 1:537841/140‑1 (MQ=255) gACTTTGTGCATAAGTTTGTTTTTTGTTTTTG‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCatat < 2:46761/140‑1 (MQ=255) tttttgtttttggGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCa < 2:772217/140‑1 (MQ=255) tgtttttg‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTgg > 2:183644/1‑140 (MQ=255) gtttttg‑‑gGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGt < 2:466241/138‑1 (MQ=255) | CAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTT‑‑GGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGT > NC_000913/1093213‑1093485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |