Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,492	(T)7→8	pseudogene (892/2648 nt)	ycgH →	putative transporter component, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,485	1	.	T	100.0%	231.2 / NA	65	pseudogene (885/2648 nt)	ycgH	putative transporter component, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (35/30);  total (35/30)

ATTATGTGGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTA‑TTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGATAGTATTGATGT  >  NC_000913/1220346‑1220616                                                                                                                                             |                                                                                                                                    aTTATGTGGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTa                                                                                                                                      >  1:763469/1‑140 (MQ=255) aTTATGTGGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTa                                                                                                                                      >  1:112769/1‑140 (MQ=255)     tgtgGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTA‑tttt                                                                                                                                 >  2:664346/1‑140 (MQ=255)     tgtgGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTA‑tttt                                                                                                                                 >  2:393224/1‑140 (MQ=255)          cACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTA‑ttttttttc                                                                                                                            >  1:655761/1‑138 (MQ=255)               gAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATg                                                                                                                        >  2:297019/1‑140 (MQ=255)                   cTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCAc                                                                                                                    <  1:517444/140‑1 (MQ=255)                    tgtgGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCAcc                                                                                                                   <  1:302394/140‑1 (MQ=255)                     gtgGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACcg                                                                                                                  <  1:297019/140‑1 (MQ=255)                       ggCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACcgcg                                                                                                                >  1:37523/1‑140 (MQ=255)                       ggCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACcgcg                                                                                                                >  1:16487/1‑140 (MQ=255)                       ggCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACcgcg                                                                                                                >  1:530817/1‑140 (MQ=255)                                aaGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCaaaa                                                                                                       <  2:763469/140‑1 (MQ=255)                                 aGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAAc                                                                                                      >  1:47833/1‑140 (MQ=255)                                      tGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGAt                                                                                                 >  2:470948/1‑140 (MQ=255)                                        cAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATaa                                                                                               <  1:704652/140‑1 (MQ=255)                                          gTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAg                                                                                             <  2:112769/140‑1 (MQ=255)                                           ttGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAgg                                                                                            >  2:603837/1‑140 (MQ=255)                                            tGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGa                                                                                           <  2:37523/140‑1 (MQ=255)                                                aTTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAatat                                                                                       >  1:83716/1‑140 (MQ=255)                                                    gACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAAt                                                                                   <  1:470948/140‑1 (MQ=255)                                                    gACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAG‑CCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAAtt                                                                                  >  2:703411/1‑140 (MQ=255)                                                        aGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGAt                                                                               <  2:47833/140‑1 (MQ=255)                                                           tAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCa                                                                            <  2:729018/140‑1 (MQ=255)                                                                   atatAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAatat                                                                    >  2:402097/1‑140 (MQ=255)                                                                   atatAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAatat                                                                    >  1:526102/1‑140 (MQ=255)                                                                   atatAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAatat                                                                    >  1:538671/1‑140 (MQ=255)                                                                   atatAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAatat                                                                    >  2:578667/1‑140 (MQ=255)                                                                    tataAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGTAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATc                                                                   >  1:246039/1‑140 (MQ=255)                                                                    tataAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATc                                                                   >  1:667874/1‑140 (MQ=255)                                                                    tataAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATc                                                                   >  1:442794/1‑140 (MQ=255)                                                                     ataAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCg                                                                  <  2:526102/140‑1 (MQ=255)                                                                     ataAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCg                                                                  >  1:639683/1‑140 (MQ=255)                                                                        aaaaaTAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGAtt                                                               >  2:180191/1‑140 (MQ=255)                                                                         aaaaTAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATtc                                                              >  2:464444/1‑140 (MQ=255)                                                                          aaaTAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATtct                                                             <  1:21477/140‑1 (MQ=255)                                                                               gTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGc                                                        <  1:578667/140‑1 (MQ=255)                                                                               gTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGc                                                        <  1:402097/140‑1 (MQ=255)                                                                                ttCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGcc                                                       >  2:637799/1‑140 (MQ=255)                                                                                  cTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCaa                                                     <  1:114837/140‑1 (MQ=255)                                                                                     ttACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAAt                                                  >  2:434048/1‑140 (MQ=255)                                                                                       acaacaacaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAg                                                <  1:443975/140‑1 (MQ=255)                                                                                       acaacaacaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAg                                                <  1:637799/140‑1 (MQ=255)                                                                                        caacaacaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGc                                               <  2:667874/140‑1 (MQ=255)                                                                                         aacaacaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCa                                              <  2:83716/140‑1 (MQ=255)                                                                                           caacaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACt                                            >  1:96688/1‑140 (MQ=255)                                                                                             acaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATTTCGATTCTCAAGCCAAAATa                                                 >  2:679053/1‑133 (MQ=255)                                                                                             acaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATTTCGATTCTCAAGCCAAAATa                                                 <  1:679053/133‑1 (MQ=255)                                                                                             acaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATa                                                 <  1:1227/133‑1 (MQ=255)                                                                                             acaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATa                                                 >  2:1227/1‑133 (MQ=255)                                                                                             acaGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTAt                                          >  2:229219/1‑140 (MQ=255)                                                                                              caGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTAtt                                         >  2:41180/1‑140 (MQ=255)                                                                                               aGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCaaaa                                                   >  1:526749/1‑129 (MQ=255)                                                                                               aGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCaaaa                                                   <  2:526749/129‑1 (MQ=255)                                                                                                     tAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGtt                                  >  1:214402/1‑140 (MQ=255)                                                                                                      aaaGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTg                                 >  2:441612/1‑140 (MQ=255)                                                                                                            cccATTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACttt                           >  1:384457/1‑140 (MQ=255)                                                                                                               aTTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        <  1:703411/140‑1 (MQ=255)                                                                                                               aTTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        <  2:230852/140‑1 (MQ=255)                                                                                                               aTTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        >  1:230852/1‑140 (MQ=255)                                                                                                               aTTGCAAAAATATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        <  1:434048/140‑1 (MQ=255)                                                                                                                       aaTATACAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTg                <  1:603837/140‑1 (MQ=255)                                                                                                                         tataCAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGa              <  1:41180/140‑1 (MQ=255)                                                                                                                          ataCAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGAt             <  2:655761/140‑1 (MQ=255)                                                                                                                          ataCAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGAt             <  1:98096/140‑1 (MQ=255)                                                                                                                           taCAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        <  1:231163/128‑1 (MQ=255)                                                                                                                           taCAATGGTGGAGAGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCaa                        >  2:231163/1‑128 (MQ=255)                                                                                                                                     gagaGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGATAGTATTGATGt  >  1:253364/1‑140 (MQ=255)                                                                                                                                     gagaGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGATAGTATTGATGt  >  1:733633/1‑140 (MQ=255)                                                                                                                                     gagaGTTATTTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGATAGTATTGATGt  >  1:91574/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                    ATTATGTGGCACTTGAAACTGTGGCTGATGGAAGTTATGCAGTTGCTATTCGACAAGGTAACATTGATATAAAAAATAGTTCTATTACAACAACAGGCACTAAAGCCCCCATTGCAAAAATATACAATGGTGGAGAGTTA‑TTTTTTTCCAATGTCACCGCGGTATCAAAACAAGATAAAGGAATATCAATTGATGCATCAAATATCGATTCTCAAGCCAAAATAGCACTATTAAGTGTTGAACTTTCAAGTGCTTTGGATAGTATTGATGT  >  NC_000913/1220346‑1220616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑