Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,768,131	(C)6→7	coding (555/3057 nt)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,768,125	1	.	C	100.0%	186.4 / NA	53	G187G (GGC→GGG)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/30);  total (23/30)

GGGAAGTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCG  >  NC_000913/1767988‑1768255                                                                                                                                           |                                                                                                                                    gggAAGTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAACCCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cc                                                                                                                                  <  2:606335/140‑1 (MQ=255) gggAAGTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cc                                                                                                                                  <  2:290370/140‑1 (MQ=255)   gAAGTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cccc                                                                                                                                <  2:104670/140‑1 (MQ=255)      gTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑ccccccc                                                                                                                             >  1:284881/1‑139 (MQ=255)        tGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cccccccaa                                                                                                                           <  2:97472/140‑4 (MQ=255)         gTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cccccccaac                                                                                                                          >  1:560524/1‑136 (MQ=255)         gTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑cccccccaac                                                                                                                          >  1:513120/1‑136 (MQ=255)                 ttAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACcgc                                                                                                                   >  1:311618/1‑140 (MQ=255)                    aCTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTTTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACcgcgcg                                                                                                                >  2:276399/1‑140 (MQ=255)                      tGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTa                                                                                                              >  1:402727/1‑140 (MQ=255)                      tGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTa                                                                                                              <  1:490942/140‑1 (MQ=255)                        gCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACg                                                                                                            >  2:318783/1‑140 (MQ=255)                        gCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACg                                                                                                            <  2:402727/140‑1 (MQ=255)                        gCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACg                                                                                                            <  1:511533/140‑1 (MQ=255)                        gCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACg                                                                                                            >  1:727223/1‑140 (MQ=255)                          gTTGCTGACGGCAACGTTGATAAACCGTGTTATAACTTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACTcc                                                                                                          <  2:554972/140‑1 (MQ=255)                           ttGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCa                                                                                                         <  1:491210/140‑1 (MQ=255)                                gACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTAc                                                                                                    <  1:742062/140‑1 (MQ=255)                                  cGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGCCCCCCCCAACAACACCGCGCGTACGCCAAGTACg                                                                                                   <  2:680633/140‑1 (MQ=255)                                        cGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATCGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGc                                                                                                           >  2:51660/1‑125 (MQ=255)                                        cGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATCGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGc                                                                                                           <  1:51660/125‑1 (MQ=255)                                           tGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACg                                                                                         >  1:148222/1‑140 (MQ=255)                                                 aCCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGc                                                                                   >  2:117035/1‑140 (MQ=255)                                                   cGTGTTATAAATTCGCCCGATTTTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCa                                                                                 <  1:276399/140‑1 (MQ=255)                                                   cGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCa                                                                                 >  1:507268/1‑140 (MQ=255)                                                     tgtTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTAGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATa                                                                               >  2:561664/1‑140 (MQ=255)                                                            aaTTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGttt                                                                                      >  2:702610/1‑126 (MQ=255)                                                            aaTTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGCTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGttt                                                                                      <  1:702610/126‑1 (MQ=255)                                                                 gCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATcc                                                                   <  2:454756/140‑1 (MQ=255)                                                                       ttGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGa                                                                             >  1:33701/1‑124 (MQ=255)                                                                       ttGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGa                                                                             <  2:33701/124‑1 (MQ=255)                                                                       ttGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAcc                                                             >  2:352853/1‑140 (MQ=255)                                                                          tgGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGa                                                          <  2:560524/140‑1 (MQ=255)                                                                            gTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATg                                                        <  2:507268/140‑1 (MQ=255)                                                                              aTTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCa                                                      <  1:318783/140‑1 (MQ=255)                                                                              aTTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCa                                                      <  2:284881/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  1:352853/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  2:358616/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  2:295626/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  1:561664/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  2:778842/140‑1 (MQ=255)                                                                               ttGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  1:117035/140‑1 (MQ=255)                                                                                 ggATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCg                                                   >  1:623766/1‑140 (MQ=255)                                                                                    tttACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGt                                                 >  1:585780/1‑139 (MQ=255)                                                                                    tttACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGt                                                 <  2:585780/139‑1 (MQ=255)                                                                                      tACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTAtt                                              <  2:767297/140‑1 (MQ=255)                                                                                       aCCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg                                             >  1:179783/1‑140 (MQ=255)                                                                                            gCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     <  1:577192/127‑1 (MQ=255)                                                                                            gCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCAt                                                     >  2:577192/1‑127 (MQ=255)                                                                                            gCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGatcat                                        >  1:701232/1‑140 (MQ=255)                                                                                             cGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGatcatc                                       >  1:1139/1‑140 (MQ=255)                                                                                                    gCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGa                                <  2:1139/140‑1 (MQ=255)                                                                                                       aaTTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGa                                            >  2:592679/1‑125 (MQ=255)                                                                                                       aaTTCGACGGGTAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGa                                            <  1:592679/125‑1 (MQ=255)                                                                                                                  tAAAGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTg                  <  1:292233/140‑1 (MQ=255)                                                                                                                     aGGTTGCGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTg               <  2:148222/140‑1 (MQ=255)                                                                                                                           cGTATCGAGAATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGaa         >  2:577282/1‑140 (MQ=255)                                                                                                                                 gagaATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc   >  2:53394/1‑140 (MQ=255)                                                                                                                                 gagaATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc   >  2:424183/1‑140 (MQ=255)                                                                                                                                  agaATATCGC‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCg  <  2:513120/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    GGGAAGTTGTCGATAATTAACTGGCGTTGCTGACGGCAACGTTGATAAACCGTGTTATAAATTCGCCCGATTGTGGTATTGGATTTACCCAGCGTTTCCGCCAATTCGACGGGTAAAGGTTGCGTATCGAGAATATCG‑‑CCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCG  >  NC_000913/1767988‑1768255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑