Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,833,210	(C)5→6	coding (783/807 nt)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,833,205	1	.	C	100.0%	150.6 / NA	44	A260P (GCC→CCC)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/24);  total (20/24)

GCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑CCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATA  >  NC_000913/1833074‑1833334                                                                                                                                     |                                                                                                                                  gCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGCAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑ccccccgt                                                                                                                           >  2:120015/1‑137 (MQ=255) gCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑ccccccgt                                                                                                                           >  1:60511/1‑137 (MQ=255) gCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑ccccccgt                                                                                                                           >  2:219118/1‑137 (MQ=255) gCTCAAAAGGCTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑ccccccgc                                                                                                                           >  1:76604/1‑137 (MQ=255)             ttGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCtt                                                                                                                >  1:268878/1‑140 (MQ=255)                  gATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTccgcc                                                                                                           <  1:434325/140‑1 (MQ=255)                     aaCCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCt                                                                                                        >  1:801911/1‑140 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTccgcc                                                                                                           <  1:573194/125‑1 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTccgcc                                                                                                           >  2:573194/1‑125 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGct                                                                                            >  2:324945/1‑140 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGct                                                                                            >  1:354968/1‑140 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGct                                                                                            >  2:403070/1‑140 (MQ=255)                                 cgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGct                                                                                            >  1:484969/1‑140 (MQ=255)                                    aTCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCTTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCtctc                                                                                         <  2:45107/140‑1 (MQ=255)                                    aTCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCCCGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCtctc                                                                                         <  2:678763/140‑1 (MQ=255)                                     tCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTcc                                                                                        <  1:324945/140‑1 (MQ=255)                                      cGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATCTAGCAGCTCTCCt                                                                                       >  1:228858/1‑140 (MQ=255)                                         ccTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGc                                                                                    <  1:120015/140‑1 (MQ=255)                                             ctgctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCaa                                                                                <  2:763543/140‑1 (MQ=255)                                                  cGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTgg                                                                           <  1:280473/140‑1 (MQ=255)                                                   gccagccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTggg                                                                          <  1:403070/140‑1 (MQ=255)                                                    cccgccaACCGCTGGCGGAATGTTGCGTAGAATCCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGt                                                                         <  2:76604/137‑1 (MQ=255)                                                       gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAg                                                                      >  2:192975/1‑140 (MQ=255)                                                       gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAg                                                                      >  2:761455/1‑140 (MQ=255)                                                       gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAg                                                                      <  1:761455/140‑1 (MQ=255)                                                               cTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTa                                                              >  2:23986/1‑140 (MQ=255)                                                                      aaTGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTga                                                       <  2:669121/140‑1 (MQ=255)                                                                       aTGTTGCGTAGAAACCGGCATAGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTgag                                                      >  2:570732/1‑140 (MQ=255)                                                                                aGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATcaa                                             >  2:44383/1‑140 (MQ=255)                                                                                  aaaCCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATcaaca                                           <  1:192975/140‑1 (MQ=255)                                                                                    aCCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACaaa                                         >  1:248449/1‑140 (MQ=255)                                                                                            cGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACt                                       <  2:154692/134‑1 (MQ=255)                                                                                            cGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACt                                       >  1:154692/1‑134 (MQ=255)                                                                                             gACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTa                                <  1:570732/140‑1 (MQ=255)                                                                                              aCTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTaa                               >  2:16648/1‑140 (MQ=255)                                                                                                        ccGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTTGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATttgttg                     >  2:185315/1‑140 (MQ=255)                                                                                                         cGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgc                    >  1:658883/1‑140 (MQ=255)                                                                                                         cGCAGCATGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgc                    >  1:10286/1‑140 (MQ=255)                                                                                                            agcaTGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATCTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctg                 <  2:228858/140‑1 (MQ=255)                                                                                                              caTGGAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctgcc               <  2:248449/140‑1 (MQ=255)                                                                                                                 ggAAAAACCGTCCGATCACGCCCCCCGTCTTGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc            <  1:185315/140‑1 (MQ=255)                                                                                                                 ggAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc            <  2:801911/140‑1 (MQ=255)                                                                                                                 ggAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc            <  2:354968/140‑1 (MQ=255)                                                                                                                 ggAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc            <  1:44383/140‑1 (MQ=255)                                                                                                                 ggAAAAACCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTc            <  1:16648/140‑1 (MQ=255)                                                                                                                   aaaaaCCGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCt          <  2:10286/140‑1 (MQ=255)                                                                                                                        ccGTCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGtt     >  2:166793/1‑140 (MQ=255)                                                                                                                           tCCGATCACGCCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTtata  <  1:351805/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  GCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACG‑CCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATA  >  NC_000913/1833074‑1833334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑