| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,012,563 | (G)6→8 | pseudogene (62/279 nt) | intG → | putative defective phage integrase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,012,557 | 1 | . | G | 100.0% | 134.7 / NA | 39 | pseudogene (56/279 nt) | intG | putative defective phage integrase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (18/21); total (18/21) | |||||||||||
| * | NC_000913 | 2,012,557 | 2 | . | G | 94.9% | 126.6 / 0.2 | 39 | pseudogene (56/279 nt) | intG | putative defective phage integrase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (17/20); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑GGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAA > NC_000913/2012428‑2012686 || cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑ggggggggtt > 2:366885/1‑136 (MQ=255)cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑ggggggggtt > 1:392093/1‑136 (MQ=255)cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g < 2:159012/131‑1 (MQ=255)cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g > 1:159012/1‑131 (MQ=255) cATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑ggggggggttat < 1:580138/140‑7 (MQ=255) cATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑ggggggggttat < 1:254321/140‑7 (MQ=255) tCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATcgcg < 2:121030/140‑1 (MQ=255) tCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGtata < 1:205812/140‑1 (MQ=255) aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGa > 1:528209/1‑140 (MQ=255) aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑GGGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa > 1:469379/1‑140 (MQ=255) aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑GGGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa < 2:469379/140‑1 (MQ=255) aaaaCATCAAC‑AAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa > 1:788041/1‑140 (MQ=255) aaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa < 1:235190/140‑1 (MQ=255) aaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGaaa < 1:93456/140‑1 (MQ=255) cATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAAc > 1:760752/1‑140 (MQ=255) aacaaAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTc < 1:245753/140‑1 (MQ=255) gATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATc < 2:528209/140‑1 (MQ=255) ttAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAg < 1:602867/140‑1 (MQ=255) ttATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATc < 2:392093/140‑1 (MQ=255) aTCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGtt < 2:685469/124‑1 (MQ=255) aTCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGtt > 1:685469/1‑124 (MQ=255) agGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTaa > 1:357779/1‑140 (MQ=255) agGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTaa > 2:716379/1‑140 (MQ=255) tGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAAtt > 1:394315/1‑140 (MQ=255) gTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAAc < 2:106349/129‑1 (MQ=255) gTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAAc > 1:106349/1‑129 (MQ=255) gTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATtgag > 1:536835/1‑140 (MQ=255) ggTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGtt > 2:2001/1‑140 (MQ=255) ggTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGtt > 2:246138/1‑140 (MQ=255) tAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGccc < 2:788041/140‑1 (MQ=255) acaTCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCccatcc < 2:394315/140‑1 (MQ=255) acaTCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCccatcc < 1:366885/140‑1 (MQ=255) gACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGccc > 2:102171/1‑129 (MQ=255) gACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGccc < 1:102171/129‑1 (MQ=255) cGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCa < 1:8260/140‑1 (MQ=255) gtgATGTGTCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCAcgcg < 1:246138/140‑1 (MQ=255) gtCAAATGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGGGGTGAGAt < 2:262814/140‑1 (MQ=255) aaTGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGa > 1:662642/1‑140 (MQ=255) tGAGACGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATg < 1:716379/140‑1 (MQ=255) gaCGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTaa > 1:414680/1‑140 (MQ=255) gaCGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCC‑‑GCCCCACGCGGTGAGATCGGATGTAAtt > 1:641580/1‑140 (MQ=255) aCGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGAt < 1:737458/130‑1 (MQ=255) aCGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGAt > 2:737458/1‑130 (MQ=255) cGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCAcgcg > 1:695927/1‑122 (MQ=255) cGCTCTGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCAcgcg < 2:695927/122‑1 (MQ=255) tGATGTTCAGGGGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTaacaa < 1:127759/140‑1 (MQ=255) || CTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑GGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAA > NC_000913/2012428‑2012686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |