Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,141	(A)8→9	coding (309/423 nt)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,133	1	.	A	100.0%	94.7 / NA	28	E101K (GAA→AAA)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (16/12);  total (16/12)

CATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAG  >  NC_000913/215016‑215262                                                                                                                       |                                                                                                                                   catTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCa                                                                                                              <  2:176757/140‑1 (MQ=255)   tttGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCAcg                                                                                                            <  1:536961/140‑1 (MQ=255)           gcagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAAAGCCCGACGACCCACGCGGCCCa                                                                                                     >  1:755971/1‑140 (MQ=255)           gcagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGAGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCAc                                                                                                    >  2:703279/1‑140 (MQ=255)            cagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCAcg                                                                                                            <  1:201738/131‑1 (MQ=255)            cagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCAcg                                                                                                            >  2:201738/1‑131 (MQ=255)                             tCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAgcg                                                                                  >  2:110486/1‑140 (MQ=255)                                    ggCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAgg                                                                              <  2:76961/137‑1 (MQ=255)                                    ggCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAgg                                                                              >  1:76961/1‑137 (MQ=255)                                         aGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATc                                                                      >  2:511740/1‑140 (MQ=255)                                           gAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGa                                                                    >  1:554755/1‑140 (MQ=255)                                               aCCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGc                                                                >  2:97649/1‑140 (MQ=255)                                                        aGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATc                                                       >  2:104917/1‑140 (MQ=255)                                                           aaCTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAg                                                    <  1:511740/140‑1 (MQ=255)                                                           aaCTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAg                                                    >  1:138126/1‑140 (MQ=255)                                                           aaCTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAg                                                    <  1:110486/140‑1 (MQ=255)                                                                aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATc                                                       >  1:788239/1‑132 (MQ=255)                                                                aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATc                                                       <  2:788239/132‑1 (MQ=255)                                                                aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGa                                               >  1:503906/1‑140 (MQ=255)                                                                               ctGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCa                                <  2:354790/140‑1 (MQ=255)                                                                                       gCTGGTGGCTATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTgcgc                        >  2:391835/1‑140 (MQ=255)                                                                                                tATGATTAAAGAATTAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCgg               <  1:391835/140‑1 (MQ=255)                                                                                                           aaTTAACAACAGAAAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag     <  2:755971/140‑1 (MQ=255)                                                                                                             ttAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaagaag  >  2:407665/1‑140 (MQ=255)                                                                                                             ttAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaaga    >  2:216/1‑138 (MQ=255)                                                                                                             ttAACAACAGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaaga    <  1:216/138‑1 (MQ=255)                                                                                                                acaacaGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAGAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag     >  2:719623/1‑134 (MQ=255)                                                                                                                acaacaGA‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag     <  1:719623/134‑1 (MQ=255)                                                                                                                       |                                                                                                                                   CATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑‑AAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAG  >  NC_000913/215016‑215262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑