Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,305,396 (C)5→6 coding (1359/1647 nt) mqo ← malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,3911.C97.4% 124.2 / ‑4.2 38A455G (GCA→GGA) mqomalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (19/18);  minor base T (0/1);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

ATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATG‑CCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGC  >  NC_000913/2305252‑2305516
                                                                                                                                            |                                                                                                                             
aTCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATg                                                                                                                                <  2:215347/140‑1 (MQ=255)
            cTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAgc                                                                                                                    >  2:683570/1‑140 (MQ=255)
            cTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAgc                                                                                                                    >  1:754184/1‑140 (MQ=255)
                 gTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCCGGAGCGCGGc                                                                                                               >  2:532989/1‑140 (MQ=255)
                   ccATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCaa                                                                                                             <  2:745101/140‑1 (MQ=255)
                              aaCGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGc                                                                                                  >  1:323110/1‑140 (MQ=255)
                                  aTCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGTCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGt                                                                                              <  2:479901/140‑1 (MQ=255)
                                   tCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTc                                                                                             >  2:132316/1‑140 (MQ=255)
                                      cTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACt                                                                                          <  2:754184/140‑1 (MQ=255)
                                        ttCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCACGGCAATGGTTCCTTGCTGGTAACTga                                                                                        >  2:362926/1‑140 (MQ=255)
                                         tCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgac                                                                                       <  2:49444/140‑1 (MQ=255)
                                           aaCGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTgacga                                                                                     <  2:256981/140‑1 (MQ=255)
                                             cGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACt                                                                                   >  2:237388/1‑140 (MQ=255)
                                                     gCCATTGCGGGCTGGAAACACGATCTCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTAcc                                                                           >  2:377587/1‑140 (MQ=255)
                                                         ttGCGGGCTGGAAACACGATCGCCAAATACCTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGa                                                                       <  2:492913/140‑1 (MQ=255)
                                                            cGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACtt                                                                                  <  1:689656/126‑1 (MQ=255)
                                                            cGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACtt                                                                                  >  2:689656/1‑126 (MQ=255)
                                                            cGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCACGGCAATGGTTCCTTGCTGGTAACTGACGACTTCAGTACCCAGACGc                                                                    <  1:362926/140‑1 (MQ=255)
                                                                cTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTa                                                                <  1:237388/140‑1 (MQ=255)
                                                                         cGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCtt                                                       >  1:25073/1‑140 (MQ=255)
                                                                         cGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCtt                                                       >  1:483214/1‑140 (MQ=255)
                                                                            tCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTtct                                                    <  2:372109/140‑1 (MQ=255)
                                                                             cGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTtctc                                                   >  1:149750/1‑140 (MQ=255)
                                                                                   aTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATc                                             <  1:683570/140‑1 (MQ=255)
                                                                                     aCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCAc                                           <  1:377587/140‑1 (MQ=255)
                                                                                        ttttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCt                                        >  2:582503/1‑140 (MQ=255)
                                                                                        ttttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCt                                        <  2:266720/140‑1 (MQ=255)
                                                                                         tttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCtt                                       >  2:463279/1‑140 (MQ=255)
                                                                                          ttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTg                                      <  2:635734/140‑1 (MQ=255)
                                                                                              agcagATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATaa                                  <  2:149750/140‑1 (MQ=255)
                                                                                                        aCATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCAcgcg                        >  2:545740/1‑140 (MQ=255)
                                                                                                             aTCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCTGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcc                   >  2:405851/1‑140 (MQ=255)
                                                                                                             aTCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcc                   >  1:285196/1‑140 (MQ=255)
                                                                                                                 gcgcggcgGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGc               >  2:652296/1‑140 (MQ=255)
                                                                                                                  cgcggcgGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCt              <  2:323110/140‑1 (MQ=255)
                                                                                                                   gcggcgGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcc                   <  1:257691/134‑1 (MQ=255)
                                                                                                                   gcggcgGTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcc                   >  2:257691/1‑134 (MQ=255)
                                                                                                                      gcggTTGACGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGcc          <  2:25073/140‑1 (MQ=255)
                                                                                                                              cGCCCCTGGCGATGCCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGc  <  1:582503/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                             
ATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATG‑CCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGC  >  NC_000913/2305252‑2305516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑