Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,486,481	(A)7→8	coding (2108/3594 nt)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,486,474	1	.	A	100.0%	245.0 / NA	69	E701K (GAA→AAA)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (37/32);  total (37/32)

ATTGATAATGGCATCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑AAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCTTAGCAAGGAG  >  NC_000913/2486336‑2486608                                                                                                                                            |                                                                                                                                       aTTGATAATGGCATCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑a                                                                                                                                       >  1:261433/1‑140 (MQ=255)  ttGATAATGGCATCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aa                                                                                                                                      >  2:209754/1‑140 (MQ=255)  ttGATAATGGCATCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aa                                                                                                                                      >  1:292468/1‑140 (MQ=255)             aTCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAg                                                                                                                            <  2:783427/140‑1 (MQ=255)                        aTGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCt                                                                                                                 >  1:96163/1‑140 (MQ=255)                         tGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTa                                                                                                                <  1:274876/140‑1 (MQ=255)                           cATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTAcc                                                                                                              <  1:614522/140‑1 (MQ=255)                                   aCTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaaa                                                                                                      >  1:786309/1‑140 (MQ=255)                                     tGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaaaaa                                                                                                    >  1:465428/1‑140 (MQ=255)                                            cATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGtt                                                                                             <  1:209754/140‑1 (MQ=255)                                                 tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTgg                                                                                        >  2:187497/1‑140 (MQ=255)                                                 tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTgg                                                                                        >  1:289287/1‑140 (MQ=255)                                                 tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTAATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTgg                                                                                        >  1:163519/1‑140 (MQ=255)                                                  gCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGc                                                                                       <  2:786309/140‑1 (MQ=255)                                                    aaTCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCaa                                                                                     <  2:193708/140‑1 (MQ=255)                                                      tCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACg                                                                                   >  2:57065/1‑140 (MQ=255)                                                       cTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa                                                                                  >  1:50216/1‑140 (MQ=255)                                                        ttCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGAt                                                                                 <  1:215115/140‑1 (MQ=255)                                                        ttCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGAt                                                                                 <  2:50216/140‑1 (MQ=255)                                                         tCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATg                                                                                >  2:144815/1‑140 (MQ=255)                                                         tCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATg                                                                                >  2:334936/1‑140 (MQ=255)                                                                             tatataTTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACAcc                                                            >  2:768916/1‑140 (MQ=255)                                                                                atatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAAt                                                         <  2:465428/140‑1 (MQ=255)                                                                                   tttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAg                                                      <  1:768916/140‑1 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     <  1:250782/140‑1 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     >  1:454502/1‑140 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     >  2:370820/1‑140 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     >  2:448090/1‑140 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     <  2:457758/140‑1 (MQ=255)                                                                                    ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc                                                     <  1:321739/140‑1 (MQ=255)                                                                                     tgtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    <  2:261433/140‑1 (MQ=255)                                                                                     tgtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    <  2:200109/140‑1 (MQ=255)                                                                                     tgtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    <  2:96163/140‑1 (MQ=255)                                                                                     tgtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    <  1:144815/140‑1 (MQ=255)                                                                                      gtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGctc                                                   >  1:8776/1‑140 (MQ=255)                                                                                       tgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAg                                                      <  1:634639/136‑1 (MQ=255)                                                                                       tgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAg                                                      >  2:634639/1‑136 (MQ=255)                                                                                       tgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGctct                                                  >  2:620745/1‑140 (MQ=255)                                                                                       tgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGctct                                                  >  1:424568/1‑140 (MQ=255)                                                                                        ggTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCta                                                 <  1:448090/140‑1 (MQ=255)                                                                                         gTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat                                                >  2:489352/1‑140 (MQ=255)                                                                                         gTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat                                                >  2:44409/1‑140 (MQ=255)                                                                                         gTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat                                                >  2:166344/1‑140 (MQ=255)                                                                                            ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAAt                                                         >  2:469898/1‑128 (MQ=255)                                                                                            ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAAt                                                         <  1:469898/128‑1 (MQ=255)                                                                                            ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtattat                                             >  2:52345/1‑140 (MQ=255)                                                                                            ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtattat                                             >  2:784864/1‑140 (MQ=255)                                                                                                 gATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCt                                        <  2:424568/140‑1 (MQ=255)                                                                                                    atTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTcc                                     >  2:36933/1‑140 (MQ=255)                                                                                                      tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    <  1:453436/123‑1 (MQ=255)                                                                                                      tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGct                                                    >  2:453436/1‑123 (MQ=255)                                                                                                       aCTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTgg                                  <  2:454502/140‑1 (MQ=255)                                                                                                       aCTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTgg                                  >  1:79864/1‑140 (MQ=255)                                                                                                           aaaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACt                              >  1:14440/1‑140 (MQ=255)                                                                                                            aaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATggg                                          >  1:565050/1‑127 (MQ=255)                                                                                                            aaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATggg                                          <  2:565050/127‑1 (MQ=255)                                                                                                               gcgTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATggg                                          <  2:233921/124‑1 (MQ=255)                                                                                                               gcgTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATggg                                          >  1:233921/1‑124 (MQ=255)                                                                                                                 gTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTc                        <  1:52345/140‑1 (MQ=255)                                                                                                                 gTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTc                        <  2:79864/140‑1 (MQ=255)                                                                                                                      cTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGtt                   <  1:489352/140‑1 (MQ=255)                                                                                                                      cTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGtt                   <  1:150016/140‑1 (MQ=255)                                                                                                                      cTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGtt                   <  1:44409/140‑1 (MQ=255)                                                                                                                              tGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCtt           >  2:755286/1‑140 (MQ=255)                                                                                                                              tGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCtt           >  1:777513/1‑140 (MQ=255)                                                                                                                               gCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCTTa          >  1:177891/1‑140 (MQ=255)                                                                                                                               gCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCGGGAACTTCTGTCGGGTTCTGATCTTa          >  2:711602/1‑140 (MQ=255)                                                                                                                                   tCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTg                <  1:280495/130‑1 (MQ=255)                                                                                                                                   tCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTg                >  2:280495/1‑130 (MQ=255)                                                                                                                                   tCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGt              >  2:568727/1‑132 (MQ=255)                                                                                                                                   tCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGt              <  1:568727/132‑1 (MQ=255)                                                                                                                                       ggTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCTTAGCAAGGAg  >  1:644511/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       ATTGATAATGGCATCGAGAAAAGATGCATTAATCACTGGCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑AAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGGCTTCCTGGAACTTCTGTCGGGTTCTGGTCTTAGCAAGGAG  >  NC_000913/2486336‑2486608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑