Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT39.3% 44.8 / 18.8 28N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (17/0);  new base T (0/11);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 4.66e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111631‑4111885
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tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                                                                                     >  1:411020/1‑140 (MQ=255)
 cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCATCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                    >  1:327319/1‑140 (MQ=255)
 cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                    >  2:1593/1‑140 (MQ=255)
       aTTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGt                                                                                                              >  2:177218/1‑140 (MQ=255)
        ttCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                                                                             >  1:383150/1‑140 (MQ=255)
             atggcgcCAGCTTAATCGTTTC‑AACAGCGAAGGTCACCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTTTAATACTGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                                                                                        <  2:327319/137‑1 (MQ=255)
                  gcCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:110376/1‑140 (MQ=255)
                  cccAGTTTAAT‑GGTTCAAACAGCGAAGGCCCCCCCTTTTTCGCCGTTGCCCGCGAAAACAAAACGCAGTGCCCCTTCTGTCTGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   <  2:614484/139‑1 (MQ=255)
                              gtttC‑AACAGGGAGGGTCCCCCCTTTTCCGCCGTTGCCCGCGACAACATACCGCAGTGCCCTTTCTGTCTGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                       <  2:383150/138‑1 (MQ=255)
                              ggTTC‑AACAGGGAAGGCCACCCCTTTTTCGCCGTGGCCCGCAACAACATAACGCTGTCCCCTTTTTTTCTGGTTTTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                       <  2:679875/140‑1 (MQ=255)
                               gttcaaaCAGCGAGGGCCACCCCTTTTCCGCCGTTGCCCGCAACAACATACCGCAGTGCCCTTTCTGTCTGGGTGTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                       <  2:140435/135‑1 (MQ=255)
                                tac‑AACAGCGAGGGCCCCCCCTTTTTCGCCGTTGCCCGCGACAACATAACGCGGTCCCCTTTCTGTCTGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     <  2:110376/138‑1 (MQ=255)
                                 ttaaaCGGCGAAGCCCACCCCTTTTCCCCCTTTGCCCGCAACAACATACCGCAGTCCACCTTCTGTCTGGGTTTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     <  2:153426/137‑1 (MQ=255)
                                 tC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    >  2:26707/1‑140 (MQ=255)
                                   aaaCAGGGAAGGCCCCCCCTTTTCCGCGGTTGTCCGCAAAAAAATACCGCGGTGCCCCTTTTGTCTGGGTGTAATATCGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAtt                                                                                   <  2:30261/139‑1 (MQ=255)
                                         cGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                                                                             >  1:430542/1‑140 (MQ=255)
                                         cGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                                                                             >  1:679875/1‑140 (MQ=255)
                                           aaGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  1:465684/1‑140 (MQ=255)
                                                         ttCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCa                                                             >  1:575408/1‑140 (MQ=255)
                                                               tttGCCCGCGACAAAATAACGCGGTGCCCCTTCTGTCTGGGTGTAATACTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  1:369729/139‑1 (MQ=255)
                                                               gTTGTCCGCAAAAAAATAACGCAGTGCCCTTTCTGTCTGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  2:32239/140‑1 (MQ=255)
                                                                        gACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTGtt                                              >  1:32239/1‑140 (MQ=255)
                                                                          caacaTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTcc                                            >  2:129029/1‑140 (MQ=255)
                                                                                  cGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgcttt                                    >  1:39490/1‑140 (MQ=255)
                                                                                       tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:555204/1‑140 (MQ=255)
                                                                                                        tttaatatCTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  2:252778/138‑1 (MQ=255)
                                                                                                        tgtAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              >  1:238344/1‑140 (MQ=255)
                                                                                                        tgtAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              >  1:252778/1‑140 (MQ=255)
                                                                                                                ttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:39490/138‑1 (MQ=255)
                                                                                                                cGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:575408/140‑1 (MQ=255)
                                                                                                                  tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:280162/1‑140 (MQ=255)
                                                                                                                  tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:780512/1‑140 (MQ=255)
                                                                                                                    tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  1:51386/1‑140 (MQ=255)
                                                                                                                    tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:81245/1‑140 (MQ=255)
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TCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC‑AACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111631‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑