Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,890	C→T	A24T (GCA→ACA)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,890	0	C	T	100.0%	164.4 / NA	47	A24T (GCA→ACA)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/25);  total (22/25)

AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTT  >  NC_000913/7757‑8018                                                                                                                                      |                                                                                                                                 aGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGaa                                                                                                                            <  2:494414/140‑1 (MQ=255) aGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACCGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGaa                                                                                                                            <  2:696829/140‑1 (MQ=255)       gTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAg                                                                                                                      >  1:349925/1‑140 (MQ=255)        tACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAgg                                                                                                                     <  2:717929/140‑1 (MQ=255)         acacAATGACTGAAGTGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGt                                                                                                                    <  1:590228/140‑1 (MQ=255)            caATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAaga                                                                                                                          >  2:650656/1‑131 (MQ=255)            caATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAaga                                                                                                                          <  1:650656/131‑1 (MQ=255)               tGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCa                                                                                                              >  1:712875/1‑140 (MQ=255)                 aCTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCAtt                                                                                                            >  1:186800/1‑140 (MQ=255)                 aCTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCAtt                                                                                                            >  2:219646/1‑140 (MQ=255)                     aaaTGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCg                                                                                                        <  2:23516/140‑1 (MQ=255)                      aaTGAGGTTACACCGGCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAACGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGGAAATCATTACCGa                                                                                                       >  2:424330/1‑140 (MQ=255)                           ggTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTa                                                                                                                   >  1:399818/1‑123 (MQ=255)                           ggTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTa                                                                                                                   <  2:399818/123‑1 (MQ=255)                            gTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCa                                                                                                 >  1:217315/1‑140 (MQ=255)                                  ccGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGAc                                                                                           <  2:597172/140‑1 (MQ=255)                                   cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                                                                          <  2:282828/140‑1 (MQ=255)                                   cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                                                                          <  2:217315/140‑1 (MQ=255)                                         gCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTa                                                                                    >  1:284610/1‑140 (MQ=255)                                                    aTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCGCAAAGGACGCTGTTAATGAAGGAGaa                                                                         >  1:562375/1‑140 (MQ=255)                                                    aTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGaa                                                                         >  2:368921/1‑140 (MQ=255)                                                       cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGaaaaa                                                                      >  1:661248/1‑140 (MQ=255)                                                       cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGaaaaa                                                                      >  2:200376/1‑140 (MQ=255)                                                            tttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCTCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg                                                                 <  2:712875/140‑1 (MQ=255)                                                             ttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                <  2:284610/140‑1 (MQ=255)                                                              tAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc                                                               <  2:661248/140‑1 (MQ=255)                                                               aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgca                                                              >  2:777668/1‑140 (MQ=255)                                                                aGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                             <  2:752289/140‑1 (MQ=255)                                                                      ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCatat                                                       >  2:661510/1‑140 (MQ=255)                                                                      ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCatat                                                       >  1:737602/1‑140 (MQ=255)                                                                        gCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                     <  1:200376/140‑1 (MQ=255)                                                                         ccAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATccc                                                    <  1:415616/140‑1 (MQ=255)                                                                                     aTGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCgg                                        <  1:777668/140‑1 (MQ=255)                                                                                         aGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc                                    >  2:669890/1‑140 (MQ=255)                                                                                           cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                  <  1:219646/140‑1 (MQ=255)                                                                                                   cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                          <  2:349925/140‑1 (MQ=255)                                                                                                   cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                          <  1:219642/140‑1 (MQ=255)                                                                                                   cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                          >  2:394915/1‑140 (MQ=255)                                                                                                   cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                          <  2:186800/140‑1 (MQ=255)                                                                                                          ccGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                   <  2:131310/140‑1 (MQ=255)                                                                                                           cGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTaa                  <  2:730450/140‑1 (MQ=255)                                                                                                             gTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAc                >  2:659379/1‑140 (MQ=255)                                                                                                             gTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAc                >  2:632065/1‑140 (MQ=255)                                                                                                                  aaaaGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTAc           >  2:177603/1‑140 (MQ=255)                                                                                                                        gAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTg     >  2:130473/1‑140 (MQ=255)                                                                                                                          aCCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGtt   >  2:473933/1‑140 (MQ=255)                                                                                                                           ccAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAGTGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt  <  2:339497/140‑1 (MQ=255)                                                                                                                           ccAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt  <  1:394915/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTT  >  NC_000913/7757‑8018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑