Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,680,086	G→A	T887I (ACC→ATC)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,680,086	0	G	A	100.0%	87.7 / NA	26	T887I (ACC→ATC)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

TTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTT  >  NC_000913/2679950‑2680221                                                                                                                                         |                                                                                                                                        ttGCCCCGGTAAACGGCCTTCGCTTAAATTCT‑CGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGAc                                                                                                                                     <  2:170986/140‑1 (MQ=255)  tGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGAc                                                                                                                                     <  2:722084/140‑1 (MQ=255)             aCGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCttcccttccc                                                                                                                          <  2:143554/140‑1 (MQ=255)                    tCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTc                                                                                                                   >  1:241800/1‑140 (MQ=255)                            aTTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGt                                                                                                           <  2:782399/140‑1 (MQ=255)                                tcCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGaa                                                                                                       <  1:383422/140‑1 (MQ=255)                                          gCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTg                                                                                             <  2:742043/140‑1 (MQ=255)                                                          cAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTccgccg                                                                             <  1:594504/140‑1 (MQ=255)                                                             ttCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTccgccg                                                                             >  2:755675/1‑137 (MQ=255)                                                             ttCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTccgccg                                                                             <  1:755675/137‑1 (MQ=255)                                                                     ccTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCTTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGcc                                                                  <  2:717152/140‑1 (MQ=255)                                                                      ctgctgCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCt                                                                 <  1:24662/140‑1 (MQ=255)                                                                              gCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTg                                                         >  2:307532/1‑140 (MQ=255)                                                                                       cATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCcac                                                <  1:283495/140‑1 (MQ=255)                                                                                                gctgcCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGc                                       >  1:210955/1‑140 (MQ=255)                                                                                                    ccAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGtt                                   >  2:499765/1‑140 (MQ=255)                                                                                                       ggCGCGTAATAACTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                                        >  1:744969/1‑132 (MQ=255)                                                                                                       ggCGCGTAATAACTGGTTGAGGATAAACTGACTGACGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                                        <  2:744969/132‑1 (MQ=255)                                                                                                             taataaCTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCa                          >  2:604023/1‑140 (MQ=255)                                                                                                              aataaCTGGTTGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCAt                         >  2:292272/1‑140 (MQ=255)                                                                                                                       ttGAGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTcgcg                >  2:760623/1‑140 (MQ=255)                                                                                                                          aGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGttt             >  2:150875/1‑140 (MQ=255)                                                                                                                          aGGATAAACTGACTGATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGttt             >  1:473603/1‑140 (MQ=255)                                                                                                                                 actgactgATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCa      >  1:394313/1‑140 (MQ=255)                                                                                                                                     actgATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAAttt  >  2:444089/1‑140 (MQ=255)                                                                                                                                     actgATGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAAttt  >  1:38735/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTT  >  NC_000913/2679950‑2680221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑