Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,282	A→G	S88P (TCT→CCT)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,282	0	A	G	100.0%	174.3 / NA	50	S88P (TCT→CCT)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/18);  total (32/18)

TCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCA  >  NC_000913/2695146‑2695412                                                                                                                                         |                                                                                                                                   tCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCa                                                                                                                                 >  1:365150/1‑140 (MQ=255)   gTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAgg                                                                                                                               >  2:585302/1‑140 (MQ=255)      cAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGgag                                                                                                                            >  2:360278/1‑140 (MQ=255)      cAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGgag                                                                                                                            >  2:360277/1‑140 (MQ=255)                     ctgctgCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGAc                                                                                                             <  1:397701/140‑1 (MQ=255)                        ctgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGAc                                                                                                             >  2:263745/1‑137 (MQ=255)                        ctgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGAc                                                                                                             <  1:263745/137‑1 (MQ=255)                               tGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCAc                                                                                                   <  1:272590/140‑1 (MQ=255)                                         tCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGt                                                                                         <  2:475765/140‑1 (MQ=255)                                           aGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGttt                                                                                       >  1:420595/1‑140 (MQ=255)                                             cGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGc                                                                                     >  2:194919/1‑140 (MQ=255)                                                aCCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGTCAGGACGCGGGGTCACCAGCAGGAGTTTGCCtt                                                                                  >  2:232500/1‑140 (MQ=255)                                                  cGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGc                                                                                >  2:467904/1‑140 (MQ=255)                                                       tCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGc                                                                           >  2:192050/1‑140 (MQ=255)                                                        cTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCg                                                                          <  2:365150/140‑1 (MQ=255)                                                        cTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGGCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCg                                                                          <  2:676930/140‑1 (MQ=255)                                                              aGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGtggctg                                                                    >  1:97563/1‑140 (MQ=255)                                                                tAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGtggctggc                                                                  >  1:402197/1‑140 (MQ=255)                                                                  aGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGa                                                                >  2:296128/1‑140 (MQ=255)                                                                  aGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGa                                                                >  1:341746/1‑140 (MQ=255)                                                                   gCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAg                                                               >  2:24632/1‑140 (MQ=255)                                                                     aaCGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGGCAGGGAGAGATGGTGCCAGGCCGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGcggctggccggtg                                                             >  2:339763/1‑135 (MQ=255)                                                                     aaCGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTg                                                             >  2:228745/1‑140 (MQ=255)                                                                     aaCGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTg                                                             <  2:97563/140‑1 (MQ=255)                                                                      aCGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGc                                                            <  1:228745/140‑1 (MQ=255)                                                                       cgccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                                           <  2:341746/140‑1 (MQ=255)                                                                       cgccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                                           >  1:743502/1‑140 (MQ=255)                                                                       cgccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                                           >  1:416462/1‑140 (MQ=255)                                                                       cgccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                                           >  1:49087/1‑140 (MQ=255)                                                                        gccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGGCg                                                          >  2:694511/1‑140 (MQ=255)                                                                        gccgcGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCg                                                          >  1:371721/1‑140 (MQ=255)                                                                          cgcgCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCg                                                          <  1:146626/138‑1 (MQ=255)                                                                          cgcgCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCg                                                          >  2:146626/1‑138 (MQ=255)                                                                           gcgcTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGAGGCGTGGCTGGCGAGTGCCGGGc                                                       <  1:360277/140‑1 (MQ=255)                                                                           gcgcTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGAGGCGTGGCTGGCGAGTGCCGGGc                                                       <  1:360278/140‑1 (MQ=255)                                                                                           aCCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCg                                       <  1:626364/140‑1 (MQ=255)                                                                                              tgttgtAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCTTATTTCAGCAGGCGTTc                                    >  1:632999/1‑140 (MQ=255)                                                                                                  gtAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGt                                <  1:467904/140‑1 (MQ=255)                                                                                                     gCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTtgt                             <  2:743502/140‑1 (MQ=255)                                                                                                        cGCAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgcg                          <  2:402197/140‑1 (MQ=255)                                                                                                          cAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgcgtg                        >  1:227090/1‑140 (MQ=255)                                                                                                          cAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgcgtg                        >  2:288202/1‑140 (MQ=255)                                                                                                          cAGTTATGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgcgtg                        >  1:791020/1‑140 (MQ=255)                                                                                                                tGGGCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCtcatc                  >  1:256941/1‑140 (MQ=255)                                                                                                                 gggCAATATCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGTCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCtcatca                 <  1:232500/140‑1 (MQ=255)                                                                                                                       tatCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGttt           >  1:36739/1‑140 (MQ=255)                                                                                                                       tatCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGttt           >  2:187535/1‑140 (MQ=255)                                                                                                                       tatCGGTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGttt           >  1:389482/1‑140 (MQ=255)                                                                                                                            gTCGCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACgg      >  1:763916/1‑140 (MQ=255)                                                                                                                               gCTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGgcgc   >  2:555951/1‑140 (MQ=255)                                                                                                                                cTTTCGAAGGCCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCTTATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCa  <  2:632999/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCA  >  NC_000913/2695146‑2695412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑