Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,078	G→A	G109S (GGT→AGT)	ung →	uracil‑DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,078	0	G	A	100.0%	198.3 / NA	56	G109S (GGT→AGT)	ung	uracil‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

GGATCCTTATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAA  >  NC_000913/2716942‑2717211                                                                                                                                         |                                                                                                                                      ggATCCTTATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGtt                                                                                                                                    <  1:275244/140‑1 (MQ=255) ggATCCTTATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGtt                                                                                                                                    <  1:267122/140‑1 (MQ=255)      cTTATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCtt                                                                                                                               >  1:267137/1‑140 (MQ=255)       ttATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTg                                                                                                                              >  1:557521/1‑140 (MQ=255)             cGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTAATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCt                                                                                                                        >  1:133773/1‑140 (MQ=255)                aCCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTggg                                                                                                                     >  1:722607/1‑140 (MQ=255)                aCCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTggg                                                                                                                     >  1:652318/1‑140 (MQ=255)                aCCGGGACAGGCGCATGGTCCGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTggg                                                                                                                     >  2:437396/1‑140 (MQ=255)                       cAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCa                                                                                                              <  1:502640/140‑1 (MQ=255)                          gcgcATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAggg                                                                                                           <  2:560189/140‑1 (MQ=255)                            gcATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCTCCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCg                                                                                                         <  2:15923/140‑1 (MQ=255)                            gcATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCg                                                                                                         >  2:435460/1‑140 (MQ=255)                                 gTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTg                                                                                                    <  1:699744/140‑1 (MQ=255)                                   cTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTAATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCt                                                                                                  <  2:133773/140‑1 (MQ=255)                                    tGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCt                                                                                                  <  2:405693/139‑1 (MQ=255)                                    tGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCt                                                                                                  >  1:405693/1‑139 (MQ=255)                                    tGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTa                                                                                                 >  1:339765/1‑140 (MQ=255)                                      gCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACt                                                                                               <  1:93299/140‑1 (MQ=255)                                      gCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTATGCTACt                                                                                               <  2:652318/140‑1 (MQ=255)                                          tttCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAAt                                                                                           <  2:740583/140‑1 (MQ=255)                                           ttCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATa                                                                                          >  2:522971/1‑140 (MQ=255)                                            tCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATAc                                                                                         <  1:522971/140‑1 (MQ=255)                                            tCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATAc                                                                                         >  1:134429/1‑140 (MQ=255)                                            tCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAAGACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGGGTAAGGGCGTTCTGCTACTCAAtgc                                                                                         <  2:598476/140‑3 (MQ=255)                                                      ccGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATGCTGTGTTGACg                                                                               <  2:557521/140‑1 (MQ=255)                                                      ccGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACg                                                                               <  2:267137/140‑1 (MQ=255)                                                             tGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTAcgcg                                                                        <  2:93991/140‑1 (MQ=255)                                                                         gTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGgcgc                                                            >  1:92496/1‑140 (MQ=255)                                                                               attGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTc                                                                  >  1:432468/1‑128 (MQ=255)                                                                               attGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTc                                                                  <  2:432468/128‑1 (MQ=255)                                                                                ttGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCa                                                     >  1:554238/1‑140 (MQ=255)                                                                                        gTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGcc                                             >  1:162924/1‑140 (MQ=255)                                                                                        gTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGcc                                             >  1:715150/1‑140 (MQ=255)                                                                                        gTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGcc                                             >  1:267851/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTTACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                  >  2:414793/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                  >  2:743929/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                  >  1:481171/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                  >  1:660244/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                  >  1:763435/1‑140 (MQ=255)                                                                                                   tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGACTGGGaa                                  >  2:385686/1‑140 (MQ=255)                                                                                                          tACTATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCa                           >  2:130768/1‑140 (MQ=255)                                                                                                             tATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCg                        <  1:130768/140‑1 (MQ=255)                                                                                                             tATTCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCg                        <  2:267851/140‑1 (MQ=255)                                                                                                               ttCCGGGCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGAt                      >  2:425389/1‑140 (MQ=255)                                                                                                                    ggCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCg                        >  1:724434/1‑133 (MQ=255)                                                                                                                    ggCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCg                        <  2:724434/133‑1 (MQ=255)                                                                                                                    ggCTTCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGt                 >  1:154965/1‑140 (MQ=255)                                                                                                                       ttCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTTACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGAt              <  1:414793/140‑1 (MQ=255)                                                                                                                       ttCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGAt              <  2:481171/140‑1 (MQ=255)                                                                                                                       ttCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGAt              <  1:435460/140‑1 (MQ=255)                                                                                                                       ttCACCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGAt              <  2:763435/140‑1 (MQ=255)                                                                                                                          aCCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAg           <  2:722607/140‑1 (MQ=255)                                                                                                                          aCCCGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAg           <  2:92496/140‑1 (MQ=255)                                                                                                                             cGCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCt        <  2:272976/140‑1 (MQ=255)                                                                                                                              gCCCTAATCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTg       >  1:422040/1‑140 (MQ=255)                                                                                                                                   aaTCATAGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTaa  >  2:42650/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GGATCCTTATCACGGACCGGGACAGGCGCATGGTCTGGCATTTTCCGTTCGTCCCGGCATTGCCATTCCTCCGTCATTATTGAATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAA  >  NC_000913/2716942‑2717211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑