Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,328	T→C	I185I (ATT→ATC)	yfjD →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,328	0	T	C	100.0%	211.6 / NA	60	I185I (ATT→ATC)	yfjD	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

TGGTCTGGTTGCTGAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGCCAACT  >  NC_000913/2749198‑2749462                                                                                                                                   |                                                                                                                                       tggtctggtTGCTGAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGc                                                                                                                               >  2:178668/1‑140 (MQ=255) tggtctggtTGCTGAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGc                                                                                                                               <  1:484595/140‑1 (MQ=255)      tggtTGCTGAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCa                                                                                                                          <  1:178668/140‑1 (MQ=255)              gAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATAtgc                                                                                                                  >  2:268722/1‑140 (MQ=255)               aaTGCTATCACCCGTATGCTGATGCGAATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATAtgct                                                                                                                 >  2:336756/1‑140 (MQ=255)                      tCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCg                                                                                                          >  1:473220/1‑140 (MQ=255)                         cccGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTg                                                                                                       >  2:582430/1‑140 (MQ=255)                           cGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCt                                                                                                     <  2:323363/140‑1 (MQ=255)                           cGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCt                                                                                                     <  1:268722/140‑1 (MQ=255)                             tATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGt                                                                                                            <  2:790009/131‑1 (MQ=255)                             tATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGt                                                                                                            >  1:790009/1‑131 (MQ=255)                             tATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCg                                                                                                   >  2:204700/1‑140 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTg                                                                                              >  1:301427/1‑138 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTg                                                                                              <  2:301427/138‑1 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGa                                                                                            >  2:488312/1‑140 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGa                                                                                            >  1:405003/1‑140 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGa                                                                                            >  2:633912/1‑140 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGa                                                                                            >  1:269174/1‑140 (MQ=255)                                    aTGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGa                                                                                            <  2:586995/140‑1 (MQ=255)                                       cgcATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGaaaa                                                                                         >  2:341734/1‑140 (MQ=255)                                          atgatgGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAAt                                                                                      <  2:535302/140‑1 (MQ=255)                                             atgGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGAc                                                                                   <  1:355561/140‑1 (MQ=255)                                               gggTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCg                                                                                 <  2:473220/140‑1 (MQ=255)                                                   aTCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTtga                                                                             <  1:58247/140‑1 (MQ=255)                                                    tCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTtgat                                                                            >  1:566738/1‑140 (MQ=255)                                                      aaaaCCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAAt                                                                                      >  1:777066/1‑128 (MQ=255)                                                      aaaaCCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAAt                                                                                      <  2:777066/128‑1 (MQ=255)                                                          ccGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGAcatc                                                                      >  1:630668/1‑140 (MQ=255)                                                           cGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGAcatca                                                                     <  2:405003/140‑1 (MQ=255)                                                           cGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGAcatca                                                                     <  2:269174/140‑1 (MQ=255)                                                            gATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGAcatcat                                                                    >  2:206426/1‑140 (MQ=255)                                                              tatCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACTCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATgg                                                                  >  2:555586/1‑140 (MQ=255)                                                              tatCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATgg                                                                  >  1:11159/1‑140 (MQ=255)                                                                 cGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTg                                                                <  2:11955/139‑1 (MQ=255)                                                                 cGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTg                                                                >  1:11955/1‑139 (MQ=255)                                                                   tGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCcg                                                             <  2:11159/140‑1 (MQ=255)                                                                     gTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                           <  1:85007/140‑1 (MQ=255)                                                                        aGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                           <  2:614041/137‑1 (MQ=255)                                                                        aGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                           >  1:614041/1‑137 (MQ=255)                                                                                      cAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTa                                          <  1:341734/140‑1 (MQ=255)                                                                                      cAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTa                                          <  2:145715/140‑1 (MQ=255)                                                                                         agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTg                                       >  1:536703/1‑140 (MQ=255)                                                                                         agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTg                                       >  2:142266/1‑140 (MQ=255)                                                                                          gaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGa                                      >  2:675907/1‑140 (MQ=255)                                                                                              agTTGCGCACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATc                                  <  1:204700/140‑1 (MQ=255)                                                                                                   cgcACTATCGTGCACGAATCACGCTCATAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                         >  2:139645/1‑128 (MQ=255)                                                                                                   cgcACTATCGTGCACGAATCACGCTCATAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                         <  1:139645/128‑1 (MQ=255)                                                                                                   cgcACTATCGTGCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGa                             >  2:16410/1‑140 (MQ=255)                                                                                                             tGCACGAATCACGCTCACAAATCTCCCGTCGCAATCATGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAATTTATCGGTATTGATATCAACGATGACTGGaaa                   >  2:709752/1‑140 (MQ=255)                                                                                                              gCACGAATCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAt                  <  1:336756/140‑1 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                            <  2:320/124‑1 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                            >  1:320/1‑124 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAtt             <  2:565922/139‑1 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAtt             >  1:565922/1‑139 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTc            >  1:561545/1‑140 (MQ=255)                                                                                                                    aTCACGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTc            >  1:136500/1‑140 (MQ=255)                                                                                                                       aCGCTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgc         <  2:566738/140‑1 (MQ=255)                                                                                                                          cTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGcc      <  1:582430/140‑1 (MQ=255)                                                                                                                          cTCACAAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGcc      <  1:801126/140‑1 (MQ=255)                                                                                                                              caAATCTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGCCAAct  <  1:142266/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TGGTCTGGTTGCTGAATGCTATCACCCGTATGCTGATGCGCATGATGGGTATCAAAACCGATATCGTGGTTAGCGGCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGCCAACT  >  NC_000913/2749198‑2749462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑