Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,032	T→C	R67R (CGT→CGC)	srlA →	sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,032	0	T	C	100.0%	168.9 / NA	49	R67R (CGT→CGC)	srlA	sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/29);  total (20/29)

TGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAAT  >  NC_000913/2825896‑2826164                                                                                                                                         |                                                                                                                                     tgtTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTAc                                                                                                                                   >  1:304370/1‑140 (MQ=255)    ttACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTa                                                                                                                                <  2:310651/140‑1 (MQ=255)     tACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGctacctac                                                                                                                               <  1:213396/140‑1 (MQ=255)      aCCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTa                                                                                                                                    >  1:173373/1‑134 (MQ=255)      aCCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTa                                                                                                                                    <  2:173373/134‑1 (MQ=255)           gATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTAc                                                                                                                         >  2:732323/1‑140 (MQ=255)                  aCCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTAc                                                                                                                                   >  1:429754/1‑123 (MQ=255)                  aCCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTAc                                                                                                                                   <  2:429754/123‑1 (MQ=255)                     ggCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTgg                                                                                                               <  2:304370/140‑1 (MQ=255)                       cATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCa                                                                                                             <  2:9673/140‑1 (MQ=255)                              ccGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGt                                                                                                      >  2:500048/1‑140 (MQ=255)                                   gTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCtttt                                                                                                 <  2:471016/140‑1 (MQ=255)                                             ctgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAt                                                                                       <  2:292287/140‑1 (MQ=255)                                             ctgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAt                                                                                       <  2:50448/140‑1 (MQ=255)                                             ctgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAt                                                                                       <  2:478682/140‑1 (MQ=255)                                             ctgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAt                                                                                       <  1:697558/140‑1 (MQ=255)                                             ctgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAt                                                                                       <  1:512441/140‑1 (MQ=255)                                              tgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATg                                                                                      >  2:513822/1‑140 (MQ=255)                                              tgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATg                                                                                      >  1:72235/1‑140 (MQ=255)                                                  ggTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGAccc                                                                                  <  2:51057/140‑1 (MQ=255)                                                                 aCTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCt                                                                   >  2:550271/1‑140 (MQ=255)                                                                      ttAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATg                                                              >  1:320615/1‑140 (MQ=255)                                                                            ttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                        <  1:106813/140‑1 (MQ=255)                                                                            ttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                        <  1:104250/140‑1 (MQ=255)                                                                                     aGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACaaa                                               <  1:513822/140‑1 (MQ=255)                                                                                           gTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGc                                         >  1:398743/1‑140 (MQ=255)                                                                                            tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                        >  1:643783/1‑140 (MQ=255)                                                                                            tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                        >  1:390168/1‑140 (MQ=255)                                                                                                 aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                   >  2:790760/1‑140 (MQ=255)                                                                                                 aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                   <  1:790760/140‑1 (MQ=255)                                                                                                  aCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcg                                  <  2:390168/140‑1 (MQ=255)                                                                                                    gTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcgcg                                >  2:447785/1‑140 (MQ=255)                                                                                                     ttttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcgg                               <  1:561233/140‑1 (MQ=255)                                                                                                      tttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggc                              >  2:401795/1‑140 (MQ=255)                                                                                                       ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTCCCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                             <  2:464845/140‑1 (MQ=255)                                                                                                       ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGTCGGAAAAGTACAAACCCAGCTACTACgcggcg                             <  2:320615/140‑1 (MQ=255)                                                                                                       ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                             <  2:527029/140‑1 (MQ=255)                                                                                                       ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                             <  2:147936/140‑1 (MQ=255)                                                                                                       ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                             <  1:500048/140‑1 (MQ=255)                                                                                                        tGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                            <  1:732323/140‑1 (MQ=255)                                                                                                        tGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                            <  2:643783/140‑1 (MQ=255)                                                                                                               cGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                            >  2:475905/1‑133 (MQ=255)                                                                                                               cGTTGCGCCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                            <  1:475905/133‑1 (MQ=255)                                                                                                                    cgcCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCt                <  1:694653/140‑1 (MQ=255)                                                                                                                    cgcCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCt                >  2:688112/1‑140 (MQ=255)                                                                                                                     gcCGGTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTg               <  1:550271/140‑1 (MQ=255)                                                                                                                         gTAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCAc           >  1:317428/1‑140 (MQ=255)                                                                                                                          tAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACt          >  1:491220/1‑140 (MQ=255)                                                                                                                          tAACCCTGTTTCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACt          <  2:663074/140‑1 (MQ=255)                                                                                                                                  tttCCCGCTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCaatgaat  >  2:584070/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAAT  >  NC_000913/2825896‑2826164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑