Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,321	C→T	E369K (GAA→AAA)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,321	0	C	T	100.0%	104.6 / NA	31	E369K (GAA→AAA)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/17);  total (14/17)

CACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCG  >  NC_000913/2836184‑2836448                                                                                                                                          |                                                                                                                                cacCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTtgc                                                                                                                               >  2:595738/1‑140 (MQ=255)     gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCt                                                                                                                           >  2:162127/1‑140 (MQ=255)     gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCt                                                                                                                           >  1:122685/1‑140 (MQ=255)         gTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTcg                                                                                                                       >  1:461356/1‑140 (MQ=255)            cACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCgcgc                                                                                                                    <  1:378380/140‑1 (MQ=255)             aCCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGca                                                                                                                   <  1:595738/140‑1 (MQ=255)                 cgcACCAGGCAGAAGGCATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGcaccac                                                                                                               >  2:22085/1‑140 (MQ=255)                   cACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCg                                                                                                             <  1:161886/140‑1 (MQ=255)                              aGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATcatccatc                                                                                                  <  2:270107/140‑1 (MQ=255)                              aGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATcatccatc                                                                                                  >  1:225770/1‑140 (MQ=255)                              aGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAAAATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATcatccatc                                                                                                  <  2:747018/140‑1 (MQ=255)                                  aTTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCt                                                                                              >  1:538002/1‑140 (MQ=255)                                   tttttCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCg                                                                                                <  1:384625/137‑1 (MQ=255)                                   tttttCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCg                                                                                                >  2:384625/1‑137 (MQ=255)                                      ttCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCAc                                                                                          <  2:225770/140‑1 (MQ=255)                                             ccGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg                                                                                   <  2:538002/140‑1 (MQ=255)                                             ccGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg                                                                                   <  2:122685/140‑1 (MQ=255)                                             ccGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg                                                                                   <  2:198446/140‑1 (MQ=255)                                              cgcgCCGAGACACAGCACAGGCGGAACATTCTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcgc                                                                                  <  2:492397/140‑1 (MQ=255)                                                cgcCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg                                                                                   >  2:525851/1‑137 (MQ=255)                                                cgcCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg                                                                                   <  1:525851/137‑1 (MQ=255)                                                 gcCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGt                                                                               <  1:796071/140‑1 (MQ=255)                                                             cacagGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCGTTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcgcg                                                                                 >  2:681062/1‑126 (MQ=255)                                                             cacagGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcgcg                                                                                 <  1:681062/126‑1 (MQ=255)                                                                 ggCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATcc                                                               >  2:772187/1‑140 (MQ=255)                                                                   cGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGt                                                             <  2:461356/140‑1 (MQ=255)                                                                   cGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGt                                                             >  1:591839/1‑140 (MQ=255)                                                                       aCATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGc                                                         >  1:437062/1‑140 (MQ=255)                                                                                    cgggAGGCAAAGCCAGCGCATCCGGCACATTCCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCaaa                                            <  1:22085/138‑1 (MQ=255)                                                                                      ggAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATc                                          >  1:373541/1‑140 (MQ=255)                                                                                            aaaGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAg                                    <  2:84000/140‑1 (MQ=255)                                                                                                                 tACCCCCGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGc               >  2:41032/1‑140 (MQ=255)                                                                                                                     cccGCGAACGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCtggt           >  1:712474/1‑140 (MQ=255)                                                                                                                           aaCGGCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTa     <  1:41032/140‑1 (MQ=255)                                                                                                                              ggCGCAGCATTTTGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCg  >  1:662757/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                CACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCG  >  NC_000913/2836184‑2836448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑