Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,209	A→G	T384A (ACT→GCT)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,209	0	A	G	100.0%	165.4 / NA	47	T384A (ACT→GCT)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

TGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGT  >  NC_000913/3017073‑3017344                                                                                                                                         |                                                                                                                                        tGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTa                                                                                                                                      >  2:454452/1‑140 (MQ=255) tGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTa                                                                                                                                      >  1:490948/1‑140 (MQ=255)     cTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGc                                                                                                                                  <  2:413366/140‑1 (MQ=255)            cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCTCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGAc                                                                                                                           <  1:516813/140‑1 (MQ=255)            cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGAc                                                                                                                           <  1:126219/140‑1 (MQ=255)            cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGAc                                                                                                                           <  1:340462/140‑1 (MQ=255)            cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGAc                                                                                                                           <  1:454452/140‑1 (MQ=255)                  ccATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACctgctg                                                                                                                     >  2:450002/1‑140 (MQ=255)                           ttGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTggc                                                                                                            >  2:386107/1‑140 (MQ=255)                           ttGCAGCAGTTCTCTCTAGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTggc                                                                                                            >  1:796969/1‑140 (MQ=255)                             gcagcaGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTggcgg                                                                                                          >  1:96802/1‑140 (MQ=255)                                    ttCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTg                                                                                                   <  1:436755/140‑1 (MQ=255)                                     tctctctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTgc                                                                                                  >  1:364510/1‑140 (MQ=255)                                     tctctctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTAGCGGCTACCTgc                                                                                                  >  2:223545/1‑140 (MQ=255)                                              cgcCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTg                                                                                         >  1:563957/1‑140 (MQ=255)                                                  tttGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTg                                                                                     <  1:745869/140‑1 (MQ=255)                                                     gACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGAATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCAt                                                                                  >  2:560230/1‑140 (MQ=255)                                                         gCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATgcgc                                                                              <  2:624355/140‑1 (MQ=255)                                                             aCTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGc                                                                          >  1:615296/1‑140 (MQ=255)                                                             aCTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGc                                                                          >  2:566046/1‑140 (MQ=255)                                                                  ccATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGaa                                                                     >  2:55558/1‑140 (MQ=255)                                                                      ttCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCt                                                                 >  2:667366/1‑140 (MQ=255)                                                                          tATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGa                                                             <  1:450002/140‑1 (MQ=255)                                                                           aTTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGAc                                                            >  2:319270/1‑140 (MQ=255)                                                                            ttCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACg                                                           >  1:186005/1‑140 (MQ=255)                                                                             tCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGc                                                          <  2:215393/140‑1 (MQ=255)                                                                                ggtggtGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTg                                                       >  2:362504/1‑140 (MQ=255)                                                                                   ggtgCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTgggg                                                    <  1:560230/140‑1 (MQ=255)                                                                                          gTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGAc                                             <  2:96802/140‑1 (MQ=255)                                                                                               cTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGt                                        >  1:92319/1‑140 (MQ=255)                                                                                                         gcgATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGaa                              >  1:743610/1‑140 (MQ=255)                                                                                                         gcgATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGaa                              <  1:79776/140‑1 (MQ=255)                                                                                                         gcgATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGaa                              <  2:441227/140‑1 (MQ=255)                                                                                                           gATATTTTTGATACAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACg                            <  2:186005/140‑1 (MQ=255)                                                                                                                       aCAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACt                >  2:172237/1‑140 (MQ=255)                                                                                                                       aCAGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACt                >  2:436171/1‑140 (MQ=255)                                                                                                                         aGGTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTgg              >  2:214455/1‑140 (MQ=255)                                                                                                                           gTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGaa                      >  1:671764/1‑130 (MQ=255)                                                                                                                           gTATTCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGaa                      <  2:671764/130‑1 (MQ=255)                                                                                                                              ttCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGgcagca         <  1:214455/140‑1 (MQ=255)                                                                                                                               tCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGcagcag        <  1:319270/140‑1 (MQ=255)                                                                                                                               tCGCCCTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGcagcag        >  2:144876/1‑140 (MQ=255)                                                                                                                                   ccTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGc    >  1:100619/1‑140 (MQ=255)                                                                                                                                   ccTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGc    >  1:20079/1‑140 (MQ=255)                                                                                                                                    cTATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCg   >  2:128703/1‑140 (MQ=255)                                                                                                                                     tATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGt  >  1:363744/1‑140 (MQ=255)                                                                                                                                     tATTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGt  <  2:615296/140‑1 (MQ=255)                                                                                                                                      aTTGCTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCg   <  1:745508/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGT  >  NC_000913/3017073‑3017344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑