Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,023,591	A→G	E759G (GAG→GGG)	xdhD →	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,023,591	0	A	G	100.0%	154.3 / NA	44	E759G (GAG→GGG)	xdhD	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

GGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACTGGCAGTTATAT  >  NC_000913/3023455‑3023726                                                                                                                                         |                                                                                                                                        ggTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGcc                                                                                                                                      <  2:191524/140‑1 (MQ=255)  gTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCa                                                                                                                                     >  1:157796/1‑140 (MQ=255)    gATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGt                                                                                                                                   <  1:92531/140‑1 (MQ=255)      tACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTgg                                                                                                                                 <  2:559102/140‑1 (MQ=255)       aCCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGc                                                                                                                                >  2:151765/1‑140 (MQ=255)       aCCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGc                                                                                                                                <  2:558306/140‑1 (MQ=255)       aCCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGc                                                                                                                                >  1:625171/1‑140 (MQ=255)          gATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGa                                                                                                                             >  1:125536/1‑140 (MQ=255)           aTCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGAt                                                                                                                            >  1:45023/1‑140 (MQ=255)           aTCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGAt                                                                                                                            >  1:711400/1‑140 (MQ=255)           aTCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGAt                                                                                                                            >  1:787083/1‑140 (MQ=255)            tCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATg                                                                                                                           <  2:125536/140‑1 (MQ=255)                     gttTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTgg                                                                                                                  >  1:368191/1‑140 (MQ=255)                     gttTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTgg                                                                                                                  >  1:238807/1‑140 (MQ=255)                      tttGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGc                                                                                                                 <  2:368191/140‑1 (MQ=255)                      tttGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGc                                                                                                                 >  1:678838/1‑140 (MQ=255)                           tAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGc                                                                                                            <  2:625171/140‑1 (MQ=255)                            aaaGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGcc                                                                                                           <  2:678838/140‑1 (MQ=255)                                gcgcATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGc                                                                                                       >  2:119741/1‑140 (MQ=255)                                         ccTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTgcgc                                                                                              <  1:119741/140‑1 (MQ=255)                                              tCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCaa                                                                                         >  1:757099/1‑140 (MQ=255)                                                        gCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGaa                                                                               >  2:262610/1‑140 (MQ=255)                                                               gggTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAagttagtt                                                                        >  2:519669/1‑140 (MQ=255)                                                               gggTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAagttagtt                                                                        >  1:519229/1‑140 (MQ=255)                                                                  tAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCg                                                                     <  2:519229/140‑1 (MQ=255)                                                                   aaCGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCgg                                                                    <  2:73572/140‑1 (MQ=255)                                                                   aaCGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCgg                                                                    >  1:185601/1‑140 (MQ=255)                                                                       cggcgCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGat                                                                >  1:325057/1‑140 (MQ=255)                                                                        ggcgCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGata                                                               <  2:757099/140‑1 (MQ=255)                                                                        ggcgCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGata                                                               <  1:237605/140‑1 (MQ=255)                                                                                      cGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGc                                                 >  1:146600/1‑140 (MQ=255)                                                                                                aCGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGGGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAaccggca                                       <  2:123665/140‑1 (MQ=255)                                                                                                  gggAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAaccggcacc                                     <  2:185601/140‑1 (MQ=255)                                                                                                       aaaaTTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCtt                                <  2:325057/140‑1 (MQ=255)                                                                                                          aTTCTGTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTgg                             <  1:151765/140‑1 (MQ=255)                                                                                                               gTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCTCATAAAGGCGAAACCGGCACCGGCTTTGGTTCAc                        >  2:459406/1‑140 (MQ=255)                                                                                                               gTTCCACGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCAc                        <  2:346518/140‑1 (MQ=255)                                                                                                                    aCGGCGCGCAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACtggtg                   <  2:45023/140‑1 (MQ=255)                                                                                                                        cgcgcAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGaa               >  2:602259/1‑140 (MQ=255)                                                                                                                          cgcAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACt             >  1:69038/1‑140 (MQ=255)                                                                                                                          cgcAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACt             <  1:86427/140‑1 (MQ=255)                                                                                                                           gcAAACGTTGGGTGGGCCAGTGGCAGTTGTTCAACTGGCAACGCCGGGCGTCGTGTGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGACACCGGCACCGGCTTTGGTTCACTGGTGGGAACTg            <  2:711400/140‑1 (MQ=255)                                                                                                                            cAAATGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACTgg           <  2:69038/140‑1 (MQ=255)                                                                                                                             aaaTGTTGGGTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACTGGc          <  1:58213/140‑1 (MQ=255)                                                                                                                                  aggggTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTCTCGGGGATATCGCCCATAAAGGCGAAACCGCTTCCGGCTTTGGTTCACTGGTGGCAACTGGCAGTta     <  1:48470/138‑1 (MQ=255)                                                                                                                                     ggTGGGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACTGGCAGTTatat  >  2:421698/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTTGGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGGTTCACTGGTGGGAACTGGCAGTTATAT  >  NC_000913/3023455‑3023726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑