Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,144,028	T→C	N46S (AAC→AGC)	hybB ←	hydrogenase 2 membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,144,028	0	T	C	100.0%	151.4 / NA	43	N46S (AAC→AGC)	hybB	hydrogenase 2 membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/27);  total (16/27)

ACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAC  >  NC_000913/3143898‑3144160                                                                                                                                   |                                                                                                                                     ccGCACCAGCGGAGGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCag                                                                                                                             <  1:436754/139‑1 (MQ=255)            gATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGccc                                                                                                                  <  1:592675/140‑1 (MQ=255)                  aTTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCg                                                                                                            <  2:543196/140‑1 (MQ=255)                   ttGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGa                                                                                                           <  1:734874/140‑1 (MQ=255)                    tGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGaa                                                                                                          <  1:307202/140‑1 (MQ=255)                         aCGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCa                                                                                                     >  1:13042/1‑140 (MQ=255)                           ggTTAAAGACGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCa                                                                                                                 <  2:121534/126‑1 (MQ=255)                           ggTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCa                                                                                                                 >  2:501973/1‑126 (MQ=255)                           ggTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCa                                                                                                                 >  1:121534/1‑126 (MQ=255)                           ggTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCa                                                                                                                 <  1:501973/126‑1 (MQ=255)                           ggTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGa                                                                                                   >  2:244626/1‑140 (MQ=255)                            gTTAAAGCCGTATCCCCCCCACGCCAGCCCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAc                                                                                                  <  2:239443/140‑1 (MQ=255)                            gTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAc                                                                                                  <  1:244626/140‑1 (MQ=255)                                     gTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACaa                                                                                         >  1:438393/1‑140 (MQ=255)                                            gCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAg                                                                                  <  2:537289/140‑1 (MQ=255)                                             cccACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAt                                                                                        >  1:191598/1‑133 (MQ=255)                                             cccACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAt                                                                                        <  2:191598/133‑1 (MQ=255)                                             cccACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGc                                                                                 <  1:92614/140‑1 (MQ=255)                                             cccACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGc                                                                                 <  1:560646/140‑1 (MQ=255)                                                         cccAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACg                                                                     >  2:707842/1‑140 (MQ=255)                                                              ccgccACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTaa                                                                <  2:13042/140‑1 (MQ=255)                                                                  cacaGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGt                                                            >  1:457443/1‑140 (MQ=255)                                                                  cacaGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGt                                                            >  2:102229/1‑140 (MQ=255)                                                                             ccGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCAt                                                 <  1:707842/140‑1 (MQ=255)                                                                             ccGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCAt                                                 <  1:659076/140‑1 (MQ=255)                                                                                     aaTCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGtt                                         >  2:141082/1‑140 (MQ=255)                                                                                             ggTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACtgatg                                 >  1:91689/1‑140 (MQ=255)                                                                                             ggTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACtgatg                                 >  1:202459/1‑140 (MQ=255)                                                                                                   aCGCGATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTg                           <  1:731375/140‑1 (MQ=255)                                                                                                       gATCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTgccgc                       <  1:214748/140‑1 (MQ=255)                                                                                                         tCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGccc                     <  2:276569/140‑1 (MQ=255)                                                                                                         tCCACACGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGccc                     <  1:102229/140‑1 (MQ=255)                                                                                                           cacaCGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAg                   <  2:438393/140‑1 (MQ=255)                                                                                                              acGCCCCACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCgg                <  2:77747/140‑1 (MQ=255)                                                                                                                 ccccACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTtg             <  2:91689/140‑1 (MQ=255)                                                                                                                 ccccACGGGAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTtg             <  2:602310/140‑1 (MQ=255)                                                                                                                        ggAAGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCa      <  1:141082/140‑1 (MQ=255)                                                                                                                          aaGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAg                   >  2:138334/1‑125 (MQ=255)                                                                                                                          aaGCCGCCGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAg                   <  1:138334/125‑1 (MQ=255)                                                                                                                            gccgccGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCa      >  1:11816/1‑136 (MQ=255)                                                                                                                            gccgccGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCa      <  2:11816/136‑1 (MQ=255)                                                                                                                            gccgccGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAc  >  2:394227/1‑140 (MQ=255)                                                                                                                            gccgccGCTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAc  >  1:743806/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     ACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACGGTCCAAAAATCATGACCGGTTTACTGATGATTTTGCCGCCCAGCGGTTGTGGATCATGAC  >  NC_000913/3143898‑3144160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑