Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,590	G→A	N269N (AAC→AAT)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,590	0	G	A	100.0%	165.3 / NA	47	N269N (AAC→AAT)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

TCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCC  >  NC_000913/3174465‑3174725                                                                                                                              |                                                                                                                                        tCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTc                                                                                                                           >  1:192604/1‑140 (MQ=255)  cTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCg                                                                                                                          >  2:19200/1‑140 (MQ=255)    tCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGt                                                                                                                        >  1:302629/1‑140 (MQ=255)       gccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTcag                                                                                                                     >  2:289580/1‑140 (MQ=255)                  tACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGc                                                                                                          >  2:528591/1‑140 (MQ=255)                   acacCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGcc                                                                                                         <  1:289580/140‑1 (MQ=255)                        gcgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCg                                                                                                    <  2:192604/140‑1 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTGACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  1:532071/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  1:123039/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  2:566710/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  2:516612/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  2:406431/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   <  2:394734/140‑1 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  1:674081/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   <  1:566710/140‑1 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  1:412614/1‑140 (MQ=255)                         cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCgg                                                                                                   >  1:272009/1‑140 (MQ=255)                          gcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGc                                                                                                  <  1:516612/140‑1 (MQ=255)                          gcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGc                                                                                                  <  1:528591/140‑1 (MQ=255)                             gCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGc                                                                                               <  1:406431/140‑1 (MQ=255)                               aGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCca                                                                                             <  2:412614/140‑1 (MQ=255)                                  atatTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAg                                                                                          >  2:55555/1‑140 (MQ=255)                                        cGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAgcgc                                                                                    >  2:59019/1‑140 (MQ=255)                                         ggTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                   <  2:674081/140‑1 (MQ=255)                                         ggTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                   <  1:59019/140‑1 (MQ=255)                                          gTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                                  <  1:622598/140‑1 (MQ=255)                                           tATTCACTGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCa                                                                                 >  2:279922/1‑140 (MQ=255)                                             ttCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGt                                                                               >  1:45534/1‑140 (MQ=255)                                               cacaGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTcc                                                                             <  1:55555/140‑1 (MQ=255)                                                acaGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                            <  2:396408/140‑1 (MQ=255)                                                  aGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                            >  1:406402/1‑138 (MQ=255)                                                  aGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                            <  2:406402/138‑1 (MQ=255)                                                    aaCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                                        <  1:19200/140‑1 (MQ=255)                                                     aCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGCCCACAGCt                                                                       >  2:471878/1‑140 (MQ=255)                                                         aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                   >  1:304281/1‑124 (MQ=255)                                                         aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                   <  2:304281/124‑1 (MQ=255)                                                         aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                                        <  2:225275/135‑1 (MQ=255)                                                         aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                                        >  1:225275/1‑135 (MQ=255)                                                          cGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGt                                                                  <  2:272009/140‑1 (MQ=255)                                                                                                   ggTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                                  >  1:171082/1‑131 (MQ=255)                                                                                                   ggTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                                  <  2:171082/131‑1 (MQ=255)                                                                                                   ggTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                                  >  1:122470/1‑131 (MQ=255)                                                                                                   ggTACCGCCCTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGttttt                                  <  2:122470/131‑1 (MQ=255)                                                                                                          cccTGCATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAg                  >  1:380021/1‑140 (MQ=255)                                                                                                               cATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCAt             >  1:89625/1‑140 (MQ=255)                                                                                                               cATCGTTGGGATAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCAt             >  1:388488/1‑140 (MQ=255)                                                                                                                          tAAGATTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTcc  <  2:123039/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                        TCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCC  >  NC_000913/3174465‑3174725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑