Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,470	G→A	L149L (CTG→CTA)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,470	0	G	A	100.0%	204.8 / NA	58	L149L (CTG→CTA)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

ATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATG  >  NC_000913/3208339‑3208605                                                                                                                                    |                                                                                                                                        aTTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGc                                                                                                                                 <  1:211674/140‑1 (MQ=255) aTTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGc                                                                                                                                 <  2:407485/140‑1 (MQ=255) aTTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCCGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGc                                                                                                                                 <  2:593812/140‑1 (MQ=255)   tttCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGc                                                                                                                                 >  1:543428/1‑138 (MQ=255)   tttCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGc                                                                                                                                 <  2:543428/138‑1 (MQ=255)     tCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCTGAGCTAATCCTCGCACCt                                                                                                                             >  1:711135/1‑140 (MQ=255)           ccTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAaca                                                                                                                       <  1:774476/140‑1 (MQ=255)                            ggCTATGAAAAAACCGGGCTTGGACGCCTCATTGCGGTGATTCTCGTGAAAAAGATGGGCCATCGCACGTTGTTTCTCGGCTATGCGGTGATGTTCTCGGAGCTAATCCTCGCACCTGTATCACCGTCCAACTCGGcgcg                                                                                                      <  2:801448/140‑1 (MQ=255)                            ggCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  1:332351/140‑1 (MQ=255)                            ggCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  1:787322/140‑1 (MQ=255)                            ggCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  1:295159/140‑1 (MQ=255)                             gCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGTACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACGGTCCAACTCGGCGCgt                                                                                                     >  2:216104/1‑140 (MQ=255)                             gCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  1:304863/139‑1 (MQ=255)                             gCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      >  2:304863/1‑139 (MQ=255)                             gCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  2:165433/139‑1 (MQ=255)                             gCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      >  1:165433/1‑139 (MQ=255)                                aTGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCgtggt                                                                                                  >  1:138945/1‑140 (MQ=255)                                 tGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCgtggtg                                                                                                 >  2:605131/1‑140 (MQ=255)                                  gAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGc                                                                                                >  1:532828/1‑140 (MQ=255)                                   aaaaaaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCg                                                                                               <  1:699691/140‑1 (MQ=255)                                      aaaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCgggg                                                                                            <  2:138945/140‑1 (MQ=255)                                       aaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGc                                                                                                >  2:756769/1‑135 (MQ=255)                                       aaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGc                                                                                                <  1:756769/135‑1 (MQ=255)                                       aaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCggggc                                                                                           >  1:801448/1‑139 (MQ=255)                                       aaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGa                                                                                           <  2:542337/140‑1 (MQ=255)                                       aaCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGa                                                                                           >  1:228053/1‑140 (MQ=255)                                           gggCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      >  2:801616/1‑125 (MQ=255)                                           gggCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGcgcg                                                                                                      <  1:801616/125‑1 (MQ=255)                                              cTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCgtggtg                                                                                                 >  1:373176/1‑127 (MQ=255)                                              cTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCgtggtg                                                                                                 <  2:373176/127‑1 (MQ=255)                                                    cgccgcATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGTACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACGGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCcat                                                                              <  1:216104/140‑1 (MQ=255)                                                       cgcATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCcatcat                                                                           >  2:774236/1‑140 (MQ=255)                                                                       tGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGGTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCg                                                           <  2:733046/140‑1 (MQ=255)                                                                        ggTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGc                                                          >  1:492744/1‑140 (MQ=255)                                                                         gTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCTGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGct                                                         <  2:711135/140‑1 (MQ=255)                                                                         gTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGct                                                         <  1:605131/140‑1 (MQ=255)                                                                             aaaaGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTat                                                     >  2:489230/1‑140 (MQ=255)                                                                                 gATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTatcaat                                                 <  1:465719/140‑1 (MQ=255)                                                                                        cATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAAcc                                          >  2:605524/1‑140 (MQ=255)                                                                                        cATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAAcc                                          >  2:659300/1‑140 (MQ=255)                                                                                        cATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAAcc                                          >  2:793255/1‑140 (MQ=255)                                                                                               cGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGAc                                   <  2:492744/140‑1 (MQ=255)                                                                                                     ttCTCGGCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGt                             <  1:714312/140‑1 (MQ=255)                                                                                                          ggCTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTcgcg                        <  2:319838/140‑1 (MQ=255)                                                                                                            cTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCgca                      >  2:551020/1‑140 (MQ=255)                                                                                                            cTATGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCgca                      >  1:118544/1‑140 (MQ=255)                                                                                                              aTGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTcgcg                        <  2:512897/136‑1 (MQ=255)                                                                                                              aTGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTcgcg                        >  1:512897/1‑136 (MQ=255)                                                                                                               tGCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCgcagca                   >  1:417811/1‑140 (MQ=255)                                                                                                                gCGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCAt                  <  1:774236/140‑1 (MQ=255)                                                                                                                 cGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCAtt                 <  1:551020/140‑1 (MQ=255)                                                                                                                 cGGTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCAtt                 <  1:605524/140‑1 (MQ=255)                                                                                                                   gTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTgg               <  1:793255/140‑1 (MQ=255)                                                                                                                   gTGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTgg               <  1:659300/140‑1 (MQ=255)                                                                                                                    tGATGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGc              >  1:630732/1‑140 (MQ=255)                                                                                                                       tGTTCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCg           <  2:417811/140‑1 (MQ=255)                                                                                                                         ttCTCCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTa         >  1:749076/1‑140 (MQ=255)                                                                                                                           ctcCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTcgcg                        >  2:563355/1‑123 (MQ=255)                                                                                                                           ctcCGAGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTcgcg                        <  1:563355/123‑1 (MQ=255)                                                                                                                                aGCTAATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCAtg  >  1:343651/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        ATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATG  >  NC_000913/3208339‑3208605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑