Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,233,438	A→T	I592F (ATC→TTC)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,233,438	0	A	T	100.0%	187.9 / NA	53	I592F (ATC→TTC)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/30);  total (23/30)

CAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCG  >  NC_000913/3233303‑3233572                                                                                                                                        |                                                                                                                                       cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTccc                                                                                                                                    >  2:213177/1‑140 (MQ=255) cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTccc                                                                                                                                    >  1:779427/1‑140 (MQ=255) cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTccc                                                                                                                                    <  1:559632/140‑1 (MQ=255)    ggAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAgg                                                                                                                                 <  1:372155/140‑1 (MQ=255)       aTGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGt                                                                                                                              <  1:213177/140‑1 (MQ=255)               cccccGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATc                                                                                                                      >  2:189228/1‑140 (MQ=255)                ccccGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCa                                                                                                                     >  1:109999/1‑140 (MQ=255)                ccccGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCa                                                                                                                     >  2:547463/1‑140 (MQ=255)                     tAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCGGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCACGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCagaaga                                                                                                                >  2:553182/1‑140 (MQ=255)                         ccACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGa                                                                                                            <  1:108616/140‑1 (MQ=255)                               cAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGa                                                                                                      <  2:779427/140‑1 (MQ=255)                               cAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGa                                                                                                      <  1:547463/140‑1 (MQ=255)                                  aTTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACg                                                                                                          <  2:783095/133‑1 (MQ=255)                                  aTTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACg                                                                                                          >  1:783095/1‑133 (MQ=255)                                    tgtgATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGc                                                                                                 <  2:358181/140‑1 (MQ=255)                                    tgtgATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGc                                                                                                 <  2:252509/140‑1 (MQ=255)                                       gATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAAGCGTAAGTTATCAGAAGATTGACg                                                                                                          >  2:290565/1‑128 (MQ=255)                                       gATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAAGCGTAAGTTATCAGAAGATTGACg                                                                                                          <  1:290565/128‑1 (MQ=255)                                         tGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAt                                                                                            >  2:263212/1‑140 (MQ=255)                                            tCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAtgtg                                                                                         >  1:497837/1‑140 (MQ=255)                                            tCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAtgtg                                                                                         <  1:553182/140‑1 (MQ=255)                                            tCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAtgtg                                                                                         >  1:297562/1‑140 (MQ=255)                                                 cgcAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGAt                                                                                    <  2:66071/140‑1 (MQ=255)                                                       gCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACgg                                                                              <  1:189228/140‑1 (MQ=255)                                                           gCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGaa                                                                          >  2:366062/1‑140 (MQ=255)                                                                ccAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCa                                                                     <  2:297562/140‑1 (MQ=255)                                                                      cAGGGGTTAGGCAAAACCACCGGCTGGGTCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCg                                                                            <  2:76204/127‑1 (MQ=255)                                                                      cAGGGGTTAGGCAAAACCACCGGCTGGGTCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCg                                                                            >  1:76204/1‑127 (MQ=255)                                                                        ggggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAg                                                             <  2:629264/140‑1 (MQ=255)                                                                         gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                            <  1:94913/140‑1 (MQ=255)                                                                         gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGc                                                            >  1:334311/1‑140 (MQ=255)                                                                         gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGG‑‑GTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGaaa                                                                         >  1:169765/1‑125 (MQ=255)                                                                         gggTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGG‑‑GTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCACGTGGTGATCAACGGCGaaa                                                                         <  2:169765/125‑1 (MQ=255)                                                                              aGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTgg                                                       >  2:243714/1‑140 (MQ=255)                                                                               ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                      <  2:109999/140‑1 (MQ=255)                                                                               ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                      <  2:374750/140‑1 (MQ=255)                                                                               ggCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                      <  1:684231/140‑1 (MQ=255)                                                                                 cAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                    <  2:782368/140‑1 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                      >  2:562029/1‑135 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa                                                      <  1:562029/135‑1 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                    <  1:677585/137‑1 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                    >  2:618034/1‑137 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                    <  1:618034/137‑1 (MQ=255)                                                                                    aaCCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa                                                    >  2:677585/1‑137 (MQ=255)                                                                                      ccaccGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAAtgtg                                               <  2:497837/140‑1 (MQ=255)                                                                                          cGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGa                                           >  2:70800/1‑140 (MQ=255)                                                                                           ggCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGAt                                          <  1:243714/140‑1 (MQ=255)                                                                                                gATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTgcg                                     <  1:366062/140‑1 (MQ=255)                                                                                                gATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTgcg                                     <  2:334311/140‑1 (MQ=255)                                                                                                       cGCACCACCCTGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCa                              >  1:768004/1‑140 (MQ=255)                                                                                                                 tGCTCTCGCGGGGTGTGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAAc                    >  1:21928/1‑140 (MQ=255)                                                                                                                             gtgtGAAAATGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGgcg        >  2:517715/1‑140 (MQ=255)                                                                                                                                   aaaTGTTCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCg  <  2:768004/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCG  >  NC_000913/3233303‑3233572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑