Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	282,735	T→C	V153A (GTG→GCG)	yagE →	CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	282,735	0	T	C	100.0%	134.6 / NA	40	V153A (GTG→GCG)	yagE	CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

CCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACG  >  NC_000913/282605‑282869                                                                                                                                   |                                                                                                                                       cccTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCt                                                                                                                               >  2:531835/1‑140 (MQ=255)          tGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTc                                                                                                                      >  2:303788/1‑140 (MQ=255)          tGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTc                                                                                                                      >  2:681655/1‑140 (MQ=255)           ggAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcg                                                                                                                     >  2:731252/1‑140 (MQ=255)           ggAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcg                                                                                                                     <  1:295209/140‑1 (MQ=255)             aaaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcgcg                                                                                                                   <  1:720298/140‑1 (MQ=255)             aaaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcgcg                                                                                                                   <  1:531835/140‑1 (MQ=255)              aaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcg                                                                                                                     >  1:158246/1‑137 (MQ=255)              aaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTcg                                                                                                                     <  2:158246/137‑1 (MQ=255)                 tgtCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCgcagc                                                                                                               >  1:353541/1‑140 (MQ=255)                 tgtCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCgcagc                                                                                                               >  1:753059/1‑140 (MQ=255)                      gAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAatat                                                                                                          >  2:443931/1‑140 (MQ=255)                             aCCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGc                                                                                                   >  1:421755/1‑140 (MQ=255)                                tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATc                                                                                                <  2:353541/140‑1 (MQ=255)                                 gATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCa                                                                                               <  1:443931/140‑1 (MQ=255)                                    ccGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAg                                                                                            <  1:763086/140‑1 (MQ=255)                                     cGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGa                                                                                           <  1:303788/140‑1 (MQ=255)                                                        ccGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGcc                                                                        <  2:609370/140‑1 (MQ=255)                                                          gACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCa                                                                      >  2:390459/1‑140 (MQ=255)                                                              gCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTg                                                                  >  1:233937/1‑140 (MQ=255)                                                               cGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTgc                                                                 <  2:70410/140‑1 (MQ=255)                                                                gTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTgcg                                                                <  1:681655/140‑1 (MQ=255)                                                                    cGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgcagc                                                            <  2:233937/140‑1 (MQ=255)                                                                    cGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgcagc                                                            <  1:636955/140‑1 (MQ=255)                                                                     gCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgcagca                                                           <  1:731252/140‑1 (MQ=255)                                                                                   tctATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTc                                             >  1:747337/1‑140 (MQ=255)                                                                                          cTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTg                                      <  2:753059/140‑1 (MQ=255)                                                                                             cccGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGccc                                   >  2:309001/1‑140 (MQ=255)                                                                                             cccGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGccc                                   >  2:664982/1‑140 (MQ=255)                                                                                               cGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCAt                                 <  1:390459/140‑1 (MQ=255)                                                                                                 gcgtTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATcc                               >  2:668964/1‑140 (MQ=255)                                                                                                  cgcTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCg                              <  2:421755/140‑1 (MQ=255)                                                                                                  cgcTGACCGGGCAGGATCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCg                              <  1:554832/140‑1 (MQ=255)                                                                                                                 aTCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGctc               <  1:752266/140‑1 (MQ=255)                                                                                                                 aTCTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGctc               <  2:747337/140‑1 (MQ=255)                                                                                                                   cTGACTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTg             >  1:46746/1‑140 (MQ=255)                                                                                                                      aCTCCGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCgg          >  1:263630/1‑140 (MQ=255)                                                                                                                          cGGCGCTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTac      >  2:681315/1‑140 (MQ=255)                                                                                                                            gcgcTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTacga    >  2:533491/1‑140 (MQ=255)                                                                                                                              gcTGGCGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTacgacg  <  2:754273/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACG  >  NC_000913/282605‑282869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑