Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,083	T→C	G132G (GGA→GGG)	yhcH ←	DUF386 domain‑containing protein YhcH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,083	0	T	C	100.0%	200.1 / NA	57	G132G (GGA→GGG)	yhcH	DUF386 domain‑containing protein YhcH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/26);  total (31/26)

GCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTCACACTGACG  >  NC_000913/3368947‑3369211                                                                                                                                         |                                                                                                                                 gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCt                                                                                                                               >  1:132182/1‑140 (MQ=255) gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCt                                                                                                                               <  2:207246/140‑1 (MQ=255) gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCt                                                                                                                               >  1:654212/1‑140 (MQ=255)  ccGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTATGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTg                                                                                                                              >  2:717158/1‑140 (MQ=255)  ccGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTg                                                                                                                              >  1:292810/1‑140 (MQ=255)   cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTgg                                                                                                                             >  2:782725/1‑140 (MQ=255)                 gTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAGGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCAc                                                                                                               <  2:17152/140‑1 (MQ=255)                 gTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCAc                                                                                                               <  1:777561/140‑1 (MQ=255)                    tCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTg                                                                                                            >  1:20928/1‑140 (MQ=255)                           cTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATaaa                                                                                                     <  2:654212/140‑1 (MQ=255)                              cAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAacac                                                                                                  >  1:559851/1‑140 (MQ=255)                                    gtgtCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGaa                                                                                            >  2:203410/1‑140 (MQ=255)                                     tgtCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAAc                                                                                           >  1:568627/1‑140 (MQ=255)                                          gCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTcc                                                                                      <  1:203410/140‑1 (MQ=255)                                          gCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTcc                                                                                      <  2:527136/140‑1 (MQ=255)                                             gAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCgg                                                                                   >  2:599799/1‑140 (MQ=255)                                               aTCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGtt                                                                                 >  1:411625/1‑140 (MQ=255)                                                 cTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGtttt                                                                               >  1:266593/1‑140 (MQ=255)                                                        aCGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAgatga                                                                        <  1:717158/140‑1 (MQ=255)                                                             cTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCt                                                                   <  2:6715/140‑1 (MQ=255)                                                             cTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCt                                                                   <  1:657880/140‑1 (MQ=255)                                                             cTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCt                                                                   <  2:132182/140‑1 (MQ=255)                                                               aaGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTg                                                                 <  2:544512/140‑1 (MQ=255)                                                               aaGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTg                                                                 <  2:568627/140‑1 (MQ=255)                                                                aGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGtttt                                                                               >  1:414136/1‑125 (MQ=255)                                                                aGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGtttt                                                                               <  2:414136/125‑1 (MQ=255)                                                                aGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGc                                                                <  2:20928/140‑1 (MQ=255)                                                                  ttttAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTc                                                              <  1:782725/140‑1 (MQ=255)                                                                     tAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGtt                                                           >  2:403414/1‑140 (MQ=255)                                                                       aGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTAt                                                         <  1:218329/140‑1 (MQ=255)                                                                          cATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCaa                                                      <  1:599799/140‑1 (MQ=255)                                                                            ttAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATg                                                    >  1:758547/1‑140 (MQ=255)                                                                                   aGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGtgctgc                                             <  2:411625/140‑1 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACGCGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAat                                 >  1:687615/1‑140 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTTCTGCa                                            <  2:235229/129‑1 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCa                                            >  1:235229/1‑129 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAat                                 >  2:336205/1‑140 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAat                                 >  1:570638/1‑140 (MQ=255)                                                                                               caccacAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAat                                 >  2:460826/1‑140 (MQ=255)                                                                                                   acaACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAatcatc                             <  1:403414/140‑1 (MQ=255)                                                                                                              aaTCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGa                  <  2:559851/140‑1 (MQ=255)                                                                                                              aaTCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGa                  <  2:137373/140‑1 (MQ=255)                                                                                                              aaTCTCTCCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGa                  <  2:758547/140‑1 (MQ=255)                                                                                                                  tctcCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAActc              >  1:509720/1‑140 (MQ=255)                                                                                                                   ctcCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAActct             >  2:222855/1‑140 (MQ=255)                                                                                                                   ctcCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAActct             >  2:468727/1‑140 (MQ=255)                                                                                                                   ctcCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAActct             >  2:764578/1‑140 (MQ=255)                                                                                                                    tcCAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCtt            >  1:762117/1‑140 (MQ=255)                                                                                                                     ccAGGCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTc           >  1:369148/1‑140 (MQ=255)                                                                                                                        ggCTCGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTcaca        <  1:349650/140‑1 (MQ=255)                                                                                                                            cGCCGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTCACACTGa    >  1:651868/1‑140 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCTAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTcaca        >  1:521469/1‑134 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCTAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTcaca        <  2:521469/134‑1 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGa                  <  1:28322/124‑1 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGa                  >  2:28322/1‑124 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTCACACTGAcg  >  1:391548/1‑140 (MQ=255)                                                                                                                              ccGACAACGCACCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTCACACTGAcg  >  2:352226/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 GCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGGTTTTAAGATGATGGCTTGCTCGTTATCAATGGTGCTGCAAAGCTGATAATCATCCTCATGGTGGAACTCTTCACACTGACG  >  NC_000913/3368947‑3369211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑