Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,726	A→G	V134A (GTT→GCT)	aaeA ←	aromatic carboxylic acid efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,726	0	A	G	100.0%	170.4 / NA	50	V134A (GTT→GCT)	aaeA	aromatic carboxylic acid efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

AATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACA  >  NC_000913/3388599‑3388848                                                                                                                                |                                                                                                                           aataaACTCACTGGTATAGACGTTGAGGTTGGTCACCCAGCCCTCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                <  2:742508/140‑1 (MQ=255) aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                <  1:308458/140‑1 (MQ=255)  ataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTAc                                                                                                               >  2:158637/1‑140 (MQ=255)  ataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTAc                                                                                                               >  2:108398/1‑140 (MQ=255)       cTCACCGGTATAGACGTTGAGGTTGGTCGCCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttgt                                                                                                          <  2:408729/140‑1 (MQ=255)        tCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttgtt                                                                                                         >  2:771271/1‑140 (MQ=255)          aCCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTgg                                                                                                       >  2:335215/1‑140 (MQ=255)           ccGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGc                                                                                                      <  1:108398/140‑1 (MQ=255)           ccGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGc                                                                                                      >  2:438696/1‑140 (MQ=255)            cGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGcc                                                                                                     >  2:794963/1‑140 (MQ=255)                        tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGcc                                                                                                     <  2:703552/128‑1 (MQ=255)                        tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGcc                                                                                                     >  1:703552/1‑128 (MQ=255)                        tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATctct                                                                                         >  1:434047/1‑140 (MQ=255)                        tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATctct                                                                                         >  1:661484/1‑140 (MQ=255)                         gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                        >  2:238705/1‑140 (MQ=255)                         gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                        >  1:29704/1‑140 (MQ=255)                                gTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCgaga                                                                                 >  1:778821/1‑140 (MQ=255)                                 tCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                <  1:794963/140‑1 (MQ=255)                                   aCCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACAt                                                                              <  2:434047/140‑1 (MQ=255)                                    cccAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATc                                                                             >  2:626868/1‑140 (MQ=255)                                                 tgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCga                                                                <  1:57280/140‑1 (MQ=255)                                                  ggCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgag                                                               <  1:771271/140‑1 (MQ=255)                                                  ggCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgag                                                               >  2:410080/1‑140 (MQ=255)                                                   gcgcgcGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgaga                                                              >  1:637504/1‑140 (MQ=255)                                                                   gCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCgg                                              >  1:403158/1‑140 (MQ=255)                                                                   gCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCgg                                              >  2:223149/1‑140 (MQ=255)                                                                     gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc                                            >  2:156019/1‑140 (MQ=255)                                                                     gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc                                            >  1:142861/1‑140 (MQ=255)                                                                     gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc                                            >  1:299995/1‑140 (MQ=255)                                                                         aaGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcct                                        <  1:410080/140‑1 (MQ=255)                                                                                aGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGttt                                 <  1:438696/140‑1 (MQ=255)                                                                                    ttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                             <  2:661484/140‑1 (MQ=255)                                                                                     tGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCt                            >  1:788284/1‑140 (MQ=255)                                                                                       ccAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCtgt                          >  1:704497/1‑140 (MQ=255)                                                                                       ccAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCtgt                          >  2:50887/1‑140 (MQ=255)                                                                                         aGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGc                        <  2:370936/140‑1 (MQ=255)                                                                                           aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCa                      <  2:788284/140‑1 (MQ=255)                                                                                            tCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGtt                                  >  2:295831/1‑127 (MQ=255)                                                                                            tCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGtt                                  <  1:295831/127‑1 (MQ=255)                                                                                            tCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAg                     <  2:29704/140‑1 (MQ=255)                                                                                            tCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAg                     >  1:792350/1‑140 (MQ=255)                                                                                              gcgGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTa                   <  2:792350/140‑1 (MQ=255)                                                                                                gggTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTAcc                 <  2:403158/140‑1 (MQ=255)                                                                                                 ggTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCt                <  1:50887/140‑1 (MQ=255)                                                                                                 ggTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCt                <  1:550336/140‑1 (MQ=255)                                                                                                      ccTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGataa           >  1:32270/1‑140 (MQ=255)                                                                                                          cgcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAg       <  2:704497/140‑1 (MQ=255)                                                                                                          cgcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAg       <  1:626868/140‑1 (MQ=255)                                                                                                          cgcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAg       <  2:299995/140‑1 (MQ=255)                                                                                                               tCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACa  >  1:291094/1‑140 (MQ=255)                                                                                                                                |                                                                                                                           AATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACA  >  NC_000913/3388599‑3388848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑