Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 294,167 A→G A211A (GCT→GCC)  yagM ← CP4‑6 prophage; protein YagM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913294,1670AG100.0% 157.2 / NA 46A211A (GCT→GCC) yagMCP4‑6 prophage; protein YagM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/29);  total (17/29)

AGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGT  >  NC_000913/294036‑294303
                                                                                                                                   |                                                                                                                                        
agcaTAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGccc                                                                                                                                  <  1:237431/140‑1 (MQ=255)
 gcaTAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCg                                                                                                                                 >  2:580215/1‑140 (MQ=255)
  caTAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCgg                                                                                                                                <  2:509639/140‑1 (MQ=255)
   aTAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGc                                                                                                                               >  2:149859/1‑140 (MQ=255)
     aaCCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCAt                                                                                                                             <  1:652302/140‑1 (MQ=255)
      accaccATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATc                                                                                                                            <  2:12793/140‑1 (MQ=255)
      accaccATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCA‑TTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCa                                                                                                                           <  1:126263/140‑1 (MQ=255)
        caccaTTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCaa                                                                                                                          >  2:126338/1‑140 (MQ=255)
        caccaTTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCaa                                                                                                                          <  2:220063/140‑1 (MQ=255)
             tttCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGtaa                                                                                                                     <  2:366987/140‑1 (MQ=255)
             tttCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGtaa                                                                                                                     <  2:729770/140‑1 (MQ=255)
              ttCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGtaat                                                                                                                    <  1:580215/140‑1 (MQ=255)
                cATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGtaataa                                                                                                                  <  2:643499/140‑1 (MQ=255)
                     aCATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTc                                                                                                             <  1:149859/140‑1 (MQ=255)
                         ggTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAg                                                                                                         >  1:142715/1‑140 (MQ=255)
                          gTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAgg                                                                                                        <  2:261400/140‑1 (MQ=255)
                          gTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAgg                                                                                                        <  2:142715/140‑1 (MQ=255)
                               tatCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGgttgtt                                                                                                   >  2:105796/1‑140 (MQ=255)
                                  cAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTa                                                                                                <  1:537447/140‑1 (MQ=255)
                                    aTCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATg                                                                                              <  1:126338/140‑1 (MQ=255)
                                    aTCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATg                                                                                              >  1:373628/1‑140 (MQ=255)
                                    aTCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATg                                                                                              >  1:374683/1‑140 (MQ=255)
                                    aTCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATg                                                                                              >  2:259471/1‑140 (MQ=255)
                                     tCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATga                                                                                             >  2:407898/1‑140 (MQ=255)
                                      ccAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATgaa                                                                                            <  1:54079/140‑1 (MQ=255)
                                      ccAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATgaa                                                                                            <  1:491807/140‑1 (MQ=255)
                                                       aaGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATgaat                                                                                           >  2:75659/1‑124 (MQ=255)
                                                       aaGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATgaat                                                                                           <  1:75659/124‑1 (MQ=255)
                                                       aaGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGaa                                                                           <  1:485427/140‑1 (MQ=255)
                                                        aGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAat                                                                          <  1:248410/140‑1 (MQ=255)
                                                           cATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATc                                                                       <  1:105796/140‑1 (MQ=255)
                                                              aaCAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTg                                                                    >  2:691744/1‑140 (MQ=255)
                                                                              ctAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCtt                                                    >  2:577333/1‑140 (MQ=255)
                                                                                    aCTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATc                                              >  1:578676/1‑140 (MQ=255)
                                                                                        cATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTg                                                          <  2:438857/124‑1 (MQ=255)
                                                                                        cATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTg                                                          >  1:438857/1‑124 (MQ=255)
                                                                                        cATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCaaa                                           <  1:291630/139‑1 (MQ=255)
                                                                                        cATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCaaa                                           >  2:291630/1‑139 (MQ=255)
                                                                                         aTTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAAt                                         <  2:374683/140‑1 (MQ=255)
                                                                                               cccGAATATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCttt                                   <  2:110042/140‑1 (MQ=255)
                                                                                                   aaTATAATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGAtt                               >  1:663556/1‑140 (MQ=255)
                                                                                                       taATCAATTGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATtgtaa                           <  1:447558/140‑1 (MQ=255)
                                                                                                              ttGGGATGTTGACAAGAGTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATtgtaaatgtaaa                    <  2:373628/140‑1 (MQ=255)
                                                                                                                   aTGTTTGCAAGAGGAAGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCt               <  2:621515/140‑1 (MQ=255)
                                                                                                                          cAAGAGTAGGGCCTGCCCGACATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCa        <  1:665411/140‑1 (MQ=255)
                                                                                                                              agTAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATc    <  1:511965/140‑1 (MQ=255)
                                                                                                                                tAGGGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGt  >  2:405337/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                        
AGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGT  >  NC_000913/294036‑294303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑