Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,641,125	A→G	A88A (GCA→GCG)	dtpB →	dipeptide/tripeptide:H(+) symporter DtpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,641,125	0	A	G	100.0%	137.1 / NA	41	A88A (GCA→GCG)	dtpB	dipeptide/tripeptide:H(+) symporter DtpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

AGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTG  >  NC_000913/3640992‑3641259                                                                                                                                      |                                                                                                                                       aGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtgtg                                                                                                                                  <  1:152003/140‑1 (MQ=255)   cTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                                                                >  1:716772/1‑140 (MQ=255)   cTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                                                                >  1:704414/1‑140 (MQ=255)    ttGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTg                                                                                                                               >  2:120460/1‑140 (MQ=255)    ttGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTg                                                                                                                               >  1:306262/1‑140 (MQ=255)        aTTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGa                                                                                                                           <  1:188421/140‑1 (MQ=255)             cGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGATCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                                                                      >  2:688678/1‑140 (MQ=255)             cGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                                                                      <  2:250117/140‑1 (MQ=255)              gCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCt                                                                                                                     <  1:120460/140‑1 (MQ=255)                     cAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCAt                                                                                                              >  1:566255/1‑140 (MQ=255)                      aGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                                                             >  1:52911/1‑140 (MQ=255)                        gCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGAc                                                                                                           >  2:587496/1‑140 (MQ=255)                             tGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTTGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                                                                      >  1:675841/1‑140 (MQ=255)                             tGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                                                                      >  1:735263/1‑140 (MQ=255)                                  cTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                                                                      <  2:348893/135‑1 (MQ=255)                                  cTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                                                                      >  1:348893/1‑135 (MQ=255)                                  cTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCg                                                                                                 <  2:735263/140‑1 (MQ=255)                                       ggTGCTTTTGCTGCGCTGGTCTATGGCCTCACTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACt                                                                                            <  1:168503/140‑1 (MQ=255)                                             tttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcc                                                                                      >  2:187202/1‑140 (MQ=255)                                                                   tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                                                                >  2:743273/1‑140 (MQ=255)                                                                   tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                                                                <  2:566255/140‑1 (MQ=255)                                                                   tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                                                                >  2:693119/1‑140 (MQ=255)                                                                   tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                                                                >  2:15835/1‑140 (MQ=255)                                                                   tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                                                                <  1:375325/140‑1 (MQ=255)                                                                     aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg                                                              <  1:693119/140‑1 (MQ=255)                                                                        tCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAc                                                           >  2:167788/1‑140 (MQ=255)                                                                          cATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa                                                         <  1:75107/140‑1 (MQ=255)                                                                           aTTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa                                                         <  1:301474/139‑1 (MQ=255)                                                                           aTTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa                                                         >  2:301474/1‑139 (MQ=255)                                                                             tGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCg                                                      <  1:15835/140‑1 (MQ=255)                                                                             tGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCg                                                      <  1:167788/140‑1 (MQ=255)                                                                                 ggcTATGTCGGCGATCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGt                                                  <  1:688678/140‑1 (MQ=255)                                                                                 ggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGt                                                  >  1:247395/1‑140 (MQ=255)                                                                                         cGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACg                                          >  2:460674/1‑140 (MQ=255)                                                                                             gACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCt                                      >  1:657872/1‑140 (MQ=255)                                                                                                            aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAAccc                       >  2:460286/1‑140 (MQ=255)                                                                                                            aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAAccc                       >  2:768570/1‑140 (MQ=255)                                                                                                               aaaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACccagc                    <  2:657872/140‑1 (MQ=255)                                                                                                                aaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACccagcc                   >  1:653108/1‑140 (MQ=255)                                                                                                                        aTTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCGCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAgcttgct           >  1:502020/1‑140 (MQ=255)                                                                                                                            ttCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCg       >  2:482309/1‑140 (MQ=255)                                                                                                                                 ggAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTg  >  2:95339/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTG  >  NC_000913/3640992‑3641259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑