Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,343	C→T	P340L (CCG→CTG)	yahG →	DUF1116 domain‑containing protein YahG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,343	0	C	T	100.0%	112.4 / NA	33	P340L (CCG→CTG)	yahG	DUF1116 domain‑containing protein YahG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/20);  total (13/20)

GATGCGGCGCATGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCCGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGA  >  NC_000913/339213‑339478                                                                                                                                   |                                                                                                                                        gatgCGGCGCATGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGc                                                                                                                                <  2:293646/140‑1 (MQ=255)         gcATGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTtata                                                                                                                        <  2:213716/140‑1 (MQ=255)           aTGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAg                                                                                                                      >  2:601915/1‑140 (MQ=255)            tGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGc                                                                                                                                <  2:680504/129‑1 (MQ=255)            tGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGc                                                                                                                                >  1:680504/1‑129 (MQ=255)            tGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGc                                                                                                                     >  1:295614/1‑140 (MQ=255)             ggCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAg                                                                                                                      >  2:757825/1‑138 (MQ=255)             ggCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAg                                                                                                                      <  1:757825/138‑1 (MQ=255)                aTCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGa                                                                                                                 <  2:175376/140‑1 (MQ=255)                              cGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTgg                                                                                                   >  1:405723/1‑140 (MQ=255)                                   tCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATc                                                                                              >  1:276445/1‑140 (MQ=255)                                            tGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAgc                                                                                     >  1:466450/1‑140 (MQ=255)                                            tGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAgc                                                                                     >  1:542764/1‑140 (MQ=255)                                                 cgTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCa                                                                                 >  2:580124/1‑139 (MQ=255)                                                 cgTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCa                                                                                 <  1:580124/139‑1 (MQ=255)                                                   tAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCa                                                                              >  1:784984/1‑140 (MQ=255)                                                      cGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCg                                                                           <  1:601915/140‑1 (MQ=255)                                                      cGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCg                                                                           <  2:428368/140‑1 (MQ=255)                                                          gTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAAc                                                                       >  2:228599/1‑140 (MQ=255)                                                            cGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCt                                                                     <  2:120289/140‑1 (MQ=255)                                                             gAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTa                                                                    <  2:276445/140‑1 (MQ=255)                                                              aGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTAc                                                                   <  2:316885/140‑1 (MQ=255)                                                                         cGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCgg                                                        <  2:295614/140‑1 (MQ=255)                                                                                   gCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATAGCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATg                                              <  2:311400/140‑1 (MQ=255)                                                                                                  ggTTTACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCt                               >  1:302391/1‑140 (MQ=255)                                                                                                    tttACCGGCCCGGCGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTAt                             <  2:542764/140‑1 (MQ=255)                                                                                                        ccggcccggcGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTAtcgtc                         <  2:784984/140‑1 (MQ=255)                                                                                                         cggcccggcGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTAtcgtcg                        <  2:405723/140‑1 (MQ=255)                                                                                                           gcccggcGCAGCAGGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTcgcg                      >  1:10681/1‑140 (MQ=255)                                                                                                                       agGTGATCGGCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGGCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGggcggc          <  1:343497/140‑1 (MQ=255)                                                                                                                               ggCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTATGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGga  <  2:466450/140‑1 (MQ=255)                                                                                                                               ggCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGga  <  2:10681/140‑1 (MQ=255)                                                                                                                                gCCTGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACgg      <  1:151574/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GATGCGGCGCATGGCATCGAATACAGCACCGTGGTCACCACCATGGCGCGTAACGGCGTCGAGTTCGGCCTGCGGGTCAGCGGCCTGCCGGGGCAATGGTTTACCGGCCCGGCGCAGCAGGTGATCGGCCCGATGTTTGCCGGTTATAAGCCGGAAGATTCGGGGCTGGATATCGGCGACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGA  >  NC_000913/339213‑339478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑