Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,381	A→G	S356G (AGC→GGC)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,381	0	A	G	100.0%	186.1 / NA	53	S356G (AGC→GGC)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/22);  total (31/22)

CGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCT  >  NC_000913/3932250‑3932517                                                                                                                                    |                                                                                                                                         cgcgTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAg                                                                                                                                  <  1:443937/140‑1 (MQ=255)  gcgTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGc                                                                                                                                 <  2:127043/140‑1 (MQ=255)   cgTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCa                                                                                                                                >  2:80982/1‑140 (MQ=255)                      gATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTggcggcg                                                                                                             <  2:18097/140‑1 (MQ=255)                                gCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTggcggcg                                                                                                             <  2:552459/130‑1 (MQ=255)                                gCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTggcggcg                                                                                                             >  1:552459/1‑130 (MQ=255)                                        gcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTg                                                                                           >  2:758367/1‑140 (MQ=255)                                                   acCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCAt                                                                                >  1:135354/1‑140 (MQ=255)                                                   acCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCAt                                                                                >  1:105421/1‑140 (MQ=255)                                                    ccTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATg                                                                               >  1:289471/1‑140 (MQ=255)                                                      tCCGAAATGGAGTCAGGGCCAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGGACCATGGt                                                                             >  2:562061/1‑140 (MQ=255)                                                      tCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGt                                                                             >  2:364546/1‑140 (MQ=255)                                                      tCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGt                                                                             >  1:309331/1‑140 (MQ=255)                                                      tCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGt                                                                             >  1:457910/1‑140 (MQ=255)                                                       ccGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTg                                                                            >  1:101229/1‑140 (MQ=255)                                                        cGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGc                                                                           >  2:120619/1‑140 (MQ=255)                                                         gAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCt                                                                          <  1:120619/140‑1 (MQ=255)                                                         gAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCt                                                                          <  2:480730/140‑1 (MQ=255)                                                             tGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCt                                                                          >  2:400718/1‑136 (MQ=255)                                                             tGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCt                                                                          <  1:400718/136‑1 (MQ=255)                                                                 gTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTa                                                                  <  1:364546/140‑1 (MQ=255)                                                                  tCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAt                                                                 >  1:304450/1‑140 (MQ=255)                                                                    aGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAtt                                                                >  2:684115/1‑139 (MQ=255)                                                                    aGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAtt                                                                <  1:684115/139‑1 (MQ=255)                                                                     gggCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATtct                                                              <  2:135354/140‑1 (MQ=255)                                                                           aTCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGAc                                                        >  2:683093/1‑140 (MQ=255)                                                                                   tCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTgg                                                <  2:289471/140‑1 (MQ=255)                                                                                   tCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTgg                                                <  2:105421/140‑1 (MQ=255)                                                                                     cGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCa                                              >  2:403148/1‑140 (MQ=255)                                                                                      gTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCAc                                             >  2:365538/1‑140 (MQ=255)                                                                                       tttGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGTAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACg                                            >  1:227302/1‑140 (MQ=255)                                                                                       tttGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACg                                            >  1:455573/1‑140 (MQ=255)                                                                                       tttGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACg                                            >  2:170860/1‑140 (MQ=255)                                                                                              aCTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAAc                                     <  1:758367/140‑1 (MQ=255)                                                                                                  gATGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGc                                 >  2:293441/1‑140 (MQ=255)                                                                                                    tGCTCTATGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGTAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCAc                               <  2:227302/140‑1 (MQ=255)                                                                                                          aTGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                         <  2:353375/140‑1 (MQ=255)                                                                                                          aTGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                         >  1:649607/1‑140 (MQ=255)                                                                                                          aTGTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                         <  2:457910/140‑1 (MQ=255)                                                                                                            gTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATaa                       >  1:409155/1‑140 (MQ=255)                                                                                                            gTCGCGGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATaa                       >  1:732899/1‑140 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTgg                            <  2:265865/131‑1 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTgg                            >  1:265865/1‑131 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                         >  1:64669/1‑134 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                         <  2:64669/134‑1 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTAt                   >  1:758876/1‑140 (MQ=255)                                                                                                                cgGTCGTGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTAt                   >  2:217936/1‑140 (MQ=255)                                                                                                                   tCGGGATTGTGATTGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTAtttt                >  2:735391/1‑140 (MQ=255)                                                                                                                     gtgattgtgattGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTttgt              <  2:499930/140‑1 (MQ=255)                                                                                                                     gtgattgtgattGTCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTttgt              >  1:499930/1‑140 (MQ=255)                                                                                                                         ttgtgattgtCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTgcg          <  2:610967/140‑1 (MQ=255)                                                                                                                              attgtCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGAt     >  1:106798/1‑140 (MQ=255)                                                                                                                                 gtCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCt  <  2:732899/140‑1 (MQ=255)                                                                                                                                 gtCGGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCt  <  1:170860/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCT  >  NC_000913/3932250‑3932517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑