Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,103,091	C→T	T90T (ACC→ACT)	yiiM →	2‑amino‑6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,103,091	0	C	T	100.0%	161.6 / NA	46	T90T (ACC→ACT)	yiiM	2‑amino‑6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

AGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGATATAGCGCAGT  >  NC_000913/4102964‑4103224                                                                                                                                |                                                                                                                                      aGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACg                                                                                                                           <  1:786703/140‑1 (MQ=255)                   acagaGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAg                                                                                                                      >  2:71398/1‑127 (MQ=255)                   acagaGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAg                                                                                                                      <  1:71398/127‑1 (MQ=255)                       agCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCg                                                                                                     >  2:191028/1‑140 (MQ=255)                         cgcTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGat                                                                                                   >  2:747941/1‑140 (MQ=255)                             gtgtCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATAttt                                                                                               >  1:77868/1‑140 (MQ=255)                                  aTTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGc                                                                                          <  1:511060/140‑1 (MQ=255)                                        cTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGga                                                                                    <  2:77868/140‑1 (MQ=255)                                        cTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGga                                                                                    <  2:345330/140‑1 (MQ=255)                                          cGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                                  <  1:747941/140‑1 (MQ=255)                                          cGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                                  >  1:348531/1‑140 (MQ=255)                                          cGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                                  >  1:354649/1‑140 (MQ=255)                                                         tACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGt                                                                   >  1:4430/1‑140 (MQ=255)                                                          aCTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTc                                                                  >  2:357151/1‑140 (MQ=255)                                                          aCTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTc                                                                  >  1:776964/1‑140 (MQ=255)                                                             gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagc                                                               >  1:469570/1‑140 (MQ=255)                                                              ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                              >  1:47157/1‑140 (MQ=255)                                                              ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                              >  1:488179/1‑140 (MQ=255)                                                              ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                              >  2:69695/1‑140 (MQ=255)                                                              ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                              >  1:419582/1‑140 (MQ=255)                                                                cgcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCAt                                                                             <  2:57618/123‑1 (MQ=255)                                                                cgcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCAt                                                                             >  1:57618/1‑123 (MQ=255)                                                                cgcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccag                                                            <  2:767498/140‑1 (MQ=255)                                                                cgcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccag                                                            >  1:50087/1‑140 (MQ=255)                                                                     gAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCcgcg                                                       <  2:776964/140‑1 (MQ=255)                                                                                    gCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaa                                        >  1:402477/1‑140 (MQ=255)                                                                                     cGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaaa                                       <  2:419582/140‑1 (MQ=255)                                                                                             tttgttGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAGTTa                               >  2:305328/1‑140 (MQ=255)                                                                                             tttgttGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTa                               <  1:128202/140‑1 (MQ=255)                                                                                             tttgttGCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTa                               <  2:488179/140‑1 (MQ=255)                                                                                               tgttgCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTAcc                             <  1:539202/140‑1 (MQ=255)                                                                                                  tgCGCCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCAtt                          >  1:589275/1‑140 (MQ=255)                                                                                                    cgcCTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCAtttt                        >  1:480035/1‑140 (MQ=255)                                                                                                       cTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat                     <  1:121220/140‑1 (MQ=255)                                                                                                       cTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat                     <  1:747650/140‑1 (MQ=255)                                                                                                       cTGCGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat                     <  1:69695/140‑1 (MQ=255)                                                                                                          cGTTTGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATc                  <  2:416324/140‑1 (MQ=255)                                                                                                            tttGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAg                <  2:348531/140‑1 (MQ=255)                                                                                                            tttGGTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAg                <  1:385997/140‑1 (MQ=255)                                                                                                               ggTGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGa             >  1:246728/1‑140 (MQ=255)                                                                                                                 tGAAAACCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGata           <  2:354649/140‑1 (MQ=255)                                                                                                                    aaaCCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat                     <  2:246079/127‑1 (MQ=255)                                                                                                                    aaaCCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat                     >  1:246079/1‑127 (MQ=255)                                                                                                                     aaCCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGatat                   >  2:507766/1‑128 (MQ=255)                                                                                                                     aaCCTCTCAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGatat                   <  1:507766/128‑1 (MQ=255)                                                                                                                          ctcAACTGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGATATAGCGCAGt  <  2:402477/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      AGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGATATAGCGCAGT  >  NC_000913/4102964‑4103224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑