Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,078	G→A	L280L (CTG→CTA)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,078	0	G	A	100.0%	129.6 / NA	37	L280L (CTG→CTA)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

TGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCG  >  NC_000913/371941‑372211                                                                                                                                          |                                                                                                                                      tGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGt                                                                                                                                     >  2:753830/1‑140 (MQ=255)     ggCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTg                                                                                                                                 <  1:328217/140‑1 (MQ=255)               ccGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCg                                                                                                                       >  2:393601/1‑140 (MQ=255)                 gCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCa                                                                                                                     <  1:436950/140‑1 (MQ=255)                 gCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCa                                                                                                                     >  1:798454/1‑140 (MQ=255)                 gCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCa                                                                                                                     <  1:690160/140‑1 (MQ=255)                   tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACg                                                                                                                   <  1:393601/140‑1 (MQ=255)                   tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACg                                                                                                                   <  2:675904/140‑1 (MQ=255)                   tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACg                                                                                                                   <  2:236377/140‑1 (MQ=255)                       tgCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCt                                                                                                               >  2:245903/1‑140 (MQ=255)                                        ctgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGAc                                                                                              <  2:253919/140‑1 (MQ=255)                                                   gTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATActc                                                                                   >  2:242639/1‑140 (MQ=255)                                                              ccGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTg                                                                        >  1:685980/1‑140 (MQ=255)                                                              ccGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTg                                                                        >  1:511927/1‑140 (MQ=255)                                                                ggTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTggc                                                                      <  1:753830/140‑1 (MQ=255)                                                                               atCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                       <  2:798454/140‑1 (MQ=255)                                                                                 cTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                       >  1:71408/1‑138 (MQ=255)                                                                                 cTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                       <  2:71408/138‑1 (MQ=255)                                                                                       cgACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCag                                               >  2:396966/1‑140 (MQ=255)                                                                                        gACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCaga                                              >  1:407932/1‑140 (MQ=255)                                                                                          cTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGc                                            <  2:777822/140‑1 (MQ=255)                                                                                             tgGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAgg                                         >  2:238487/1‑140 (MQ=255)                                                                                                 cACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGa                                     <  2:580879/140‑1 (MQ=255)                                                                                                       gcgcAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGAt                               >  2:784694/1‑140 (MQ=255)                                                                                                         gcAGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTg                             <  1:238487/140‑1 (MQ=255)                                                                                                           aGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCagag                                             >  2:156129/1‑122 (MQ=255)                                                                                                           aGTGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCagag                                             <  1:156129/122‑1 (MQ=255)                                                                                                             tGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCAcc                         <  1:784694/140‑1 (MQ=255)                                                                                                             tGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCAcc                         <  1:396966/140‑1 (MQ=255)                                                                                                             tGGGAACATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCAcc                         <  1:242639/140‑1 (MQ=255)                                                                                                                 aaCATGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTg                     >  2:627893/1‑140 (MQ=255)                                                                                                                     tGCCGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGcgcg                 >  1:372354/1‑140 (MQ=255)                                                                                                                       ccGACGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGAt               >  1:3783/1‑140 (MQ=255)                                                                                                                           cGCTTTCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa           <  2:3783/140‑1 (MQ=255)                                                                                                                              tttCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa           >  2:387926/1‑137 (MQ=255)                                                                                                                              tttCAATCAACTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa           <  1:387926/137‑1 (MQ=255)                                                                                                                                    tcaaCTAGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCg  >  2:60784/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCG  >  NC_000913/371941‑372211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑