Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,423,357	A→G	Y171C (TAC→TGC)	ulaE →	L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,423,357	0	A	G	100.0%	178.7 / NA	51	Y171C (TAC→TGC)	ulaE	L‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

AGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAA  >  NC_000913/4423220‑4423492                                                                                                                                          |                                                                                                                                        aGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGcg                                                                                                                                       <  2:599793/140‑1 (MQ=255)   ccAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGcgcg                                                                                                                                     >  2:713117/1‑140 (MQ=255)    cAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATgcgcgc                                                                                                                                    <  1:723889/140‑1 (MQ=255)    cAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATgcgcgc                                                                                                                                    <  1:637907/140‑1 (MQ=255)       tAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACt                                                                                                                                 >  2:433387/1‑140 (MQ=255)        aaCGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTa                                                                                                                                <  2:729193/140‑1 (MQ=255)             aaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCAc                                                                                                                                  >  1:751035/1‑133 (MQ=255)             aaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCAc                                                                                                                                  <  2:751035/133‑1 (MQ=255)             aaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTca                                                                                                                           >  1:546000/1‑140 (MQ=255)             aaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTca                                                                                                                           >  1:595847/1‑140 (MQ=255)                 cgtcgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATgcgcgc                                                                                                                                    <  2:572311/127‑1 (MQ=255)                 cgtcgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATgcgcgc                                                                                                                                    >  1:572311/1‑127 (MQ=255)                 cgtcgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGcg                                                                                                                                       >  2:316226/1‑124 (MQ=255)                 cgtcgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGcg                                                                                                                                       <  1:316226/124‑1 (MQ=255)                 cgtcgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTcaacaa                                                                                                                       >  1:700121/1‑140 (MQ=255)                     gtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCg                                                                                                                   <  2:682852/140‑1 (MQ=255)                      tcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGt                                                                                                                  <  2:369128/140‑1 (MQ=255)                              gTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAg                                                                                                          <  2:277245/140‑1 (MQ=255)                              gTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAg                                                                                                          <  2:269399/140‑1 (MQ=255)                                gACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGct                                                                                                        >  2:667555/1‑140 (MQ=255)                                     ccTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTAcc                                                                                                   >  2:112711/1‑140 (MQ=255)                                               agCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCgg                                                                                         >  2:568910/1‑140 (MQ=255)                                               agCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCgg                                                                                         >  2:129979/1‑140 (MQ=255)                                                   ttGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAAc                                                                                     >  1:674488/1‑140 (MQ=255)                                                    tGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTAc                                                                                                    <  2:796706/124‑1 (MQ=255)                                                    tGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTAc                                                                                                    >  1:796706/1‑124 (MQ=255)                                                      agaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTg                                                                                  >  2:9707/1‑140 (MQ=255)                                                       gaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGt                                                                                 <  1:9707/140‑1 (MQ=255)                                                       gaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGt                                                                                 <  2:499714/140‑1 (MQ=255)                                                       gaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTCGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGt                                                                                 <  2:700121/140‑1 (MQ=255)                                                          ggCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCgg                                                                              <  2:546000/140‑1 (MQ=255)                                                            cAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCg                                                                            <  1:41828/140‑1 (MQ=255)                                                                           tGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAtgtg                                                             <  1:429806/140‑1 (MQ=255)                                                                             aCGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCa                                                           <  1:539203/140‑1 (MQ=255)                                                                               gCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGa                                                         <  1:513094/140‑1 (MQ=255)                                                                                       tGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTg                                                 <  2:280563/140‑1 (MQ=255)                                                                                         gagaTCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCa                                               >  2:502774/1‑140 (MQ=255)                                                                                             tCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGcc                                           >  2:262633/1‑140 (MQ=255)                                                                                             tCATGGATTATCCGTCGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGcc                                           >  2:476660/1‑140 (MQ=255)                                                                                              cATGGATTATCCGTCGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCg                                          <  1:476660/140‑1 (MQ=255)                                                                                                tGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGa                                        <  1:713117/140‑1 (MQ=255)                                                                                                      aTCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCggg                                  >  2:301253/1‑140 (MQ=255)                                                                                                       tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGc                                 >  2:754001/1‑140 (MQ=255)                                                                                                        ccGTTGATGAGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCa                                >  1:313182/1‑140 (MQ=255)                                                                                                              atgaGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                          <  1:112711/140‑1 (MQ=255)                                                                                                              atgaGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                          <  1:667555/140‑1 (MQ=255)                                                                                                                gaGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg                        <  1:768223/140‑1 (MQ=255)                                                                                                                 aGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgc                       >  1:455/1‑140 (MQ=255)                                                                                                                 aGCTCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgc                       >  1:793293/1‑140 (MQ=255)                                                                                                                    tCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGt                    <  2:793293/140‑1 (MQ=255)                                                                                                                    tCCATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGt                    <  1:262633/140‑1 (MQ=255)                                                                                                                        tCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAt                <  2:313182/140‑1 (MQ=255)                                                                                                                              aGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa          <  1:754001/140‑1 (MQ=255)                                                                                                                                gcgcTGGGATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGa        >  1:367827/1‑140 (MQ=255)                                                                                                                                      ggATGCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCaa  >  1:604656/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAA  >  NC_000913/4423220‑4423492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑