Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,919	A→G	intergenic (‑239/‑11)	yjiH ← / → kptA	Gate family protein YjiH/RNA 2'‑phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,919	0	A	G	100.0%	175.0 / NA	50	intergenic (‑239/‑11)	yjiH/kptA	Gate family protein YjiH/RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

GTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGA  >  NC_000913/4560782‑4561048                                                                                                                                          |                                                                                                                                  gTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGACTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCg                                                                                                                                 >  2:800450/1‑140 (MQ=255)          tGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACgaga                                                                                                                        <  2:135814/140‑1 (MQ=255)                tGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACGTCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCaa                                                                                                                  >  2:188982/1‑140 (MQ=255)                tGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCaa                                                                                                                  >  2:698003/1‑140 (MQ=255)                                 ggAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAagag                                                                                                 <  2:235892/140‑1 (MQ=255)                                                          gCTCTGCGCGGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  1:290854/1‑140 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  1:97478/1‑140 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        <  1:764890/140‑1 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  2:506726/1‑140 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  2:519020/1‑140 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  2:713294/1‑140 (MQ=255)                                                          gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTaa                                                                        >  2:764890/1‑140 (MQ=255)                                                            tctGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGt                                                                      >  1:605015/1‑140 (MQ=255)                                                                   aGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGtcc                                                               >  2:603355/1‑140 (MQ=255)                                                                    ggATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGtcct                                                              >  2:267527/1‑140 (MQ=255)                                                                       ttGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCg                                                           <  1:519020/140‑1 (MQ=255)                                                                               cAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAAc                                                   >  2:679027/1‑140 (MQ=255)                                                                               cAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAAc                                                   >  2:476646/1‑140 (MQ=255)                                                                               cAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAAc                                                   >  1:679480/1‑140 (MQ=255)                                                                               cAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAAc                                                   >  2:121757/1‑140 (MQ=255)                                                                                    aaTTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAg                                              <  1:506726/140‑1 (MQ=255)                                                                                         aTAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                         <  2:315080/140‑1 (MQ=255)                                                                                         aTAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                         <  1:476646/140‑1 (MQ=255)                                                                                         aTAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCCCCGACACAAACCGGAGGCGAt                                         <  2:570802/140‑1 (MQ=255)                                                                                              ccAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    <  2:97478/140‑1 (MQ=255)                                                                                              ccAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    <  1:679027/140‑1 (MQ=255)                                                                                                     aCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACt                             <  2:279686/140‑1 (MQ=255)                                                                                                       aaTGACGTCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTgg                           <  1:188982/140‑1 (MQ=255)                                                                                                        aTGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGa                          <  2:656130/140‑1 (MQ=255)                                                                                                        aTGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGa                          <  1:698003/140‑1 (MQ=255)                                                                                                        aTGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGa                          <  1:247289/140‑1 (MQ=255)                                                                                                        aTGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGa                          >  1:656130/1‑140 (MQ=255)                                                                                                         tGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    >  1:582791/1‑129 (MQ=255)                                                                                                         tGACATCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    <  2:582791/129‑1 (MQ=255)                                                                                                             agcAGTGAATAAGAACCGGAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTg                     >  2:89931/3‑140 (MQ=255)                                                                                                             aTCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    <  1:672246/125‑1 (MQ=255)                                                                                                             aTCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                    >  2:672246/1‑125 (MQ=255)                                                                                                             aTCAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTg                     <  1:603355/140‑1 (MQ=255)                                                                                                               cAGTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGaa                   >  1:614784/1‑140 (MQ=255)                                                                                                                 gTAAATAAAAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAgg                 >  1:71982/1‑140 (MQ=255)                                                                                                                  taaataaaAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGa                <  2:290854/140‑1 (MQ=255)                                                                                                                   aaataaaAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGAt               >  2:427959/1‑140 (MQ=255)                                                                                                                     ataaaAACAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATgg             >  2:726774/1‑140 (MQ=255)                                                                                                                       aaaaaCAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGc           >  1:334813/1‑140 (MQ=255)                                                                                                                         aaaCAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCgg         <  1:121757/140‑1 (MQ=255)                                                                                                                            cAGAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGata      <  1:427959/140‑1 (MQ=255)                                                                                                                              gAAAATCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATc    >  1:640284/1‑140 (MQ=255)                                                                                                                                aaaTCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa  <  2:743208/140‑1 (MQ=255)                                                                                                                                aaaTCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa  <  1:726774/140‑1 (MQ=255)                                                                                                                                aaaTCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa  <  1:800450/140‑1 (MQ=255)                                                                                                                                aaaTCCAGGGGCGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa  <  1:89931/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGA  >  NC_000913/4560782‑4561048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑