Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,567,117	C→T	A44T (GCT→ACT)	yjiN ←	DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,567,117	0	C	T	100.0%	104.5 / NA	31	A44T (GCT→ACT)	yjiN	DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

ATCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATA  >  NC_000913/4566996‑4567247                                                                                                                          |                                                                                                                                   aTCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCt                                                                                                                  <  1:312708/140‑1 (MQ=255) aTCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCt                                                                                                                  <  1:611007/140‑1 (MQ=255)              taCGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTg                                                                                                     >  2:575067/1‑140 (MQ=255)               aCGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTgg                                                                                                    <  1:575067/140‑1 (MQ=255)               aCGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCACAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTgg                                                                                                    <  2:378589/140‑1 (MQ=255)                 gcgGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGggc                                                                                                  <  2:398298/140‑1 (MQ=255)                 gcgGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGggc                                                                                                  >  2:666149/1‑140 (MQ=255)                   gggATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGggcgg                                                                                                >  1:719028/1‑140 (MQ=255)                               gTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGt                                                                                    >  2:792997/1‑140 (MQ=255)                                   ggCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACg                                                                                >  2:400060/1‑140 (MQ=255)                                     cGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGAc                                                                              <  2:762068/140‑1 (MQ=255)                                                cGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTagcag                                                                   <  2:511551/140‑1 (MQ=255)                                                  gAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAgcg                                                                 >  2:689760/1‑140 (MQ=255)                                                     tCGGCACGCGGCGAAACAGCGCCACCACCGCAAGCCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAgcggcg                                                              <  2:201374/140‑1 (MQ=255)                                                     tCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAgcggcg                                                              <  1:470644/140‑1 (MQ=255)                                                       ggCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCTAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGAt                                                            <  1:110384/140‑1 (MQ=255)                                                         cACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAgcg                                                                 <  2:239270/133‑1 (MQ=255)                                                         cACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAgcg                                                                 >  1:239270/1‑133 (MQ=255)                                                          aCGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGTGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAg                                                         <  2:745519/140‑1 (MQ=255)                                                            gcggcgAAACACCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCa                                                       <  1:400060/140‑1 (MQ=255)                                                             cggcgAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAg                                                      >  2:742296/1‑140 (MQ=255)                                                                             ccaccGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAAc                                      >  1:23563/1‑140 (MQ=255)                                                                                      aCCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGgcg                             <  1:97739/140‑1 (MQ=255)                                                                                                  gcgcgcCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGTGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCg                 >  2:529572/1‑140 (MQ=255)                                                                                                   cgcgcCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGTGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGa                <  1:529572/140‑1 (MQ=255)                                                                                                   cgcgcCGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGa                <  2:113226/140‑1 (MQ=255)                                                                                                       ccGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAg            >  1:74638/1‑140 (MQ=255)                                                                                                       ccGACCATCGCCGCTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAg            >  1:14984/1‑140 (MQ=255)                                                                                                               cgccgcTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCa    <  1:666149/140‑1 (MQ=255)                                                                                                                 ccgcTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATa  <  1:792997/140‑1 (MQ=255)                                                                                                                 ccgcTTCAGTAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATa  <  1:685833/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                   ATCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATA  >  NC_000913/4566996‑4567247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑