Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,588,797 A→G H23H (CAT→CAC)  yjiA ← P‑loop guanosine triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,588,7970AG100.0% 190.1 / NA 54H23H (CAT→CAC) yjiAP‑loop guanosine triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

ACAACAGATGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATATGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAGCGCGCGTTCTGGC  >  NC_000913/4588665‑4588928
                                                                                                                                    |                                                                                                                                   
acaacaGATGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCgca                                                                                                                              <  1:222471/140‑1 (MQ=255)
     aGATGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAgg                                                                                                                         <  1:88886/140‑1 (MQ=255)
       aTGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGggt                                                                                                                       >  2:323197/1‑140 (MQ=255)
        tGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGggtg                                                                                                                      >  2:301791/1‑140 (MQ=255)
            gCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGttt                                                                                                                  >  1:139433/1‑140 (MQ=255)
                 tGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCt                                                                                                             >  1:48706/1‑140 (MQ=255)
                   gTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTgc                                                                                                           <  2:139433/140‑1 (MQ=255)
                    tCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTgcg                                                                                                          <  2:445062/140‑1 (MQ=255)
                                tCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCg                                                                                              <  2:48706/140‑1 (MQ=255)
                                  tGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCg                                                                                              >  2:568240/1‑138 (MQ=255)
                                  tGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCg                                                                                              <  1:568240/138‑1 (MQ=255)
                                  tGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGt                                                                                            <  1:4053/140‑1 (MQ=255)
                                      tGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGt                                                                                        <  1:30405/140‑1 (MQ=255)
                                         cgcgGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAgg                                                                                     >  2:311112/1‑140 (MQ=255)
                                              tCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAAc                                                                                >  2:84649/1‑140 (MQ=255)
                                              tCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAAc                                                                                >  2:151922/1‑140 (MQ=255)
                                                       aGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCgg                                                                       >  2:757964/1‑140 (MQ=255)
                                                           gATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTc                                                                   >  1:166419/1‑140 (MQ=255)
                                                                tcaACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATg                                                                <  1:219198/138‑1 (MQ=255)
                                                                tcaACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATg                                                                >  2:219198/1‑138 (MQ=255)
                                                                 caACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctc                                                             <  1:151922/140‑1 (MQ=255)
                                                                 caACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctc                                                             <  2:166419/140‑1 (MQ=255)
                                                                  aaCAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctct                                                            <  1:164977/140‑1 (MQ=255)
                                                                   aCAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctc                                                           >  2:370444/1‑140 (MQ=255)
                                                                   aCAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctc                                                           >  1:334503/1‑140 (MQ=255)
                                                                    cAGAGCCTTCGCCGAATTCGTTTTCAATCACGACAATCTTATAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACAGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          <  2:710348/140‑1 (MQ=255)
                                                                    cAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          <  1:757964/140‑1 (MQ=255)
                                                                    cAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          <  1:311112/140‑1 (MQ=255)
                                                                    cAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          <  2:377978/140‑1 (MQ=255)
                                                                      gagaCTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTcc                                                        >  2:433903/1‑140 (MQ=255)
                                                                      gagaCTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTcc                                                        >  1:233369/1‑140 (MQ=255)
                                                                       agaCTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          >  2:562151/1‑137 (MQ=255)
                                                                       agaCTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGctctct                                                          <  1:562151/137‑1 (MQ=255)
                                                                         aattCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCAtt                                                     >  1:546427/3‑140 (MQ=255)
                                                                         aCTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCAtt                                                     <  2:802773/140‑1 (MQ=255)
                                                                           ttCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTaa                                                   >  1:439467/1‑140 (MQ=255)
                                                                           ttCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTaa                                                   <  2:410825/140‑1 (MQ=255)
                                                                              gCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTaacaa                                                >  1:575150/1‑140 (MQ=255)
                                                                              gCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTaacaa                                                >  2:78085/1‑140 (MQ=255)
                                                                                 gAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAg                                             <  1:301791/140‑1 (MQ=255)
                                                                                 gAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAg                                             <  1:323197/140‑1 (MQ=255)
                                                                                  aaTTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAgc                                            >  2:392442/1‑140 (MQ=255)
                                                                                       gTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATa                                       <  2:439467/140‑1 (MQ=255)
                                                                                       gTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATa                                       <  1:779514/140‑1 (MQ=255)
                                                                                                cACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTa                              <  1:370444/140‑1 (MQ=255)
                                                                                                  cGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTAcc                            <  2:575150/140‑1 (MQ=255)
                                                                                                      aaTCTTGTAGCCGTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATcg                        <  1:433903/140‑1 (MQ=255)
                                                                                                                  gTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAgcgc            >  2:598563/1‑140 (MQ=255)
                                                                                                                  gTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAgcgc            >  2:608013/1‑140 (MQ=255)
                                                                                                                  gTGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAgcgc            <  1:78085/140‑1 (MQ=255)
                                                                                                                   tGCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTATCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTATCAACAGCGCATACGGCCTTTACCATCGCCGCCGTcgcgcg           >  2:711024/1‑140 (MQ=255)
                                                                                                                    gCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAgcgcgc          >  2:184275/1‑140 (MQ=255)
                                                                                                                    gCTGTTCGTTAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAgcgcgc          >  1:521573/1‑140 (MQ=255)
                                                                                                                            ttAAGAATGTGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCTCCGTAGCGCGCGTTCTGGc  <  1:526630/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
ACAACAGATGCAGCCGTTGGTCAGCGTTTTGATCTGCGTGGCGCGGTCGCCAATCAGTTGATCATCAACAGAGACTTCGCCGAATTCGTTTTCAATCACGGCAATCTTGTAGCCGTGCTGTTCGTTAAGAATATGGCGCAGCAGGGTGGTTTTTCCTGCGCCTAAAAAACCGGTGAGTAGGGTAACTGCAATCGGGTTCATGCTCTCTCCATTAACAACAGCGCATACCGCCTTTACCATCGCCGCCGTAGCGCGCGTTCTGGC  >  NC_000913/4588665‑4588928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑