Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,679	G→A	A174T (GCC→ACC)	bglJ →	DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,679	0	G	A	100.0%	242.3 / NA	69	A174T (GCC→ACC)	bglJ	DNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/37);  total (32/37)

TGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG  >  NC_000913/4604545‑4604815                                                                                                                                       |                                                                                                                                         tGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGag                                                                                                                                     <  1:603779/140‑1 (MQ=255)  gTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGagc                                                                                                                                    <  2:419511/140‑1 (MQ=255)       tAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCtt                                                                                                                               >  1:36873/1‑140 (MQ=255)       tAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCtt                                                                                                                               <  1:178280/140‑1 (MQ=255)               gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCaa                                                                                                                       <  1:304554/140‑1 (MQ=255)                     cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCaa                                                                                                                 >  1:174731/1‑140 (MQ=255)                      aGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCaaa                                                                                                                <  1:223617/140‑1 (MQ=255)                       ggCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCaaaa                                                                                                               >  2:38133/1‑140 (MQ=255)                        gCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAAc                                                                                                              >  1:206852/1‑140 (MQ=255)                        gCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAAc                                                                                                              >  2:322140/1‑140 (MQ=255)                             cgaccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATcc                                                                                                         >  1:743492/1‑140 (MQ=255)                                ccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTg                                                                                                      <  2:174731/140‑1 (MQ=255)                                  gacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGca                                                                                                    >  2:370404/1‑140 (MQ=255)                                    cagAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGcaca                                                                                                  <  2:743492/138‑1 (MQ=255)                                      gAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATa                                                                                                >  2:800637/1‑140 (MQ=255)                                       aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATaa                                                                                               <  2:36873/140‑1 (MQ=255)                                              aGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAt                                                                                        <  2:29283/140‑1 (MQ=255)                                                 ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGTACATAAATTTAAtgtg                                                                                     >  2:223643/1‑140 (MQ=255)                                                 ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAtgtg                                                                                     >  1:723918/1‑140 (MQ=255)                                                 ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAtgtg                                                                                     <  1:800637/140‑1 (MQ=255)                                                 ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAtgtg                                                                                     >  2:163313/1‑140 (MQ=255)                                                   tACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCAAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGAt                                                                                   >  2:408611/1‑140 (MQ=255)                                                        tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGa                                                                              >  2:373915/1‑140 (MQ=255)                                                        tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAAAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGa                                                                              <  2:645413/140‑1 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAAtt                                                                                            >  1:88097/1‑124 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAAtt                                                                                            <  2:88097/124‑1 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGaaa                                                                            >  1:582445/1‑140 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGaaa                                                                            >  1:572357/1‑140 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGaaa                                                                            >  1:800466/1‑140 (MQ=255)                                                          aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCATGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGaaa                                                                            >  2:94814/1‑140 (MQ=255)                                                             aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTg                                                                         <  2:168434/140‑1 (MQ=255)                                                                  cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGt                                                                    <  1:373915/140‑1 (MQ=255)                                                                    gACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCa                                                                  >  2:110168/1‑140 (MQ=255)                                                                        ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTc                                                              <  1:163313/140‑1 (MQ=255)                                                                             cccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg                                                         <  1:93438/140‑1 (MQ=255)                                                                              ccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc                                                        <  2:572357/140‑1 (MQ=255)                                                                                 aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                     >  2:349626/1‑140 (MQ=255)                                                                                  cGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                    <  1:110168/140‑1 (MQ=255)                                                                                          gaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                            <  2:206852/140‑1 (MQ=255)                                                                                               atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg                                       <  1:322140/140‑1 (MQ=255)                                                                                                 tGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCa                                     >  1:530933/1‑140 (MQ=255)                                                                                                 tGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCa                                     >  2:467592/1‑140 (MQ=255)                                                                                                   cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGa                                   >  1:735794/1‑140 (MQ=255)                                                                                                   cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGa                                   >  2:639258/1‑140 (MQ=255)                                                                                                    gcTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGat                                  <  2:800466/140‑1 (MQ=255)                                                                                                     cTTTATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                 >  2:313915/1‑140 (MQ=255)                                                                                                     cTTTATGTCGCATGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                 <  1:94814/140‑1 (MQ=255)                                                                                                       ttATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATAtt                               <  1:1815/140‑1 (MQ=255)                                                                                                       ttATGTCACGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAGAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATAtt                               <  1:52078/140‑1 (MQ=255)                                                                                                        tATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGatat                                >  1:441109/1‑138 (MQ=255)                                                                                                        tATGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGatat                                <  2:441109/138‑1 (MQ=255)                                                                                                          tGTCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTg                            >  2:509618/1‑140 (MQ=255)                                                                                                            tCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                          <  2:530933/140‑1 (MQ=255)                                                                                                            tCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                          <  1:514437/140‑1 (MQ=255)                                                                                                            tCGCGTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                          <  1:509618/140‑1 (MQ=255)                                                                                                              gcgTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGc                                         >  2:80955/1‑123 (MQ=255)                                                                                                              gcgTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGc                                         <  1:80955/123‑1 (MQ=255)                                                                                                               cgTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg                                    <  1:695155/127‑1 (MQ=255)                                                                                                               cgTGGCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg                                    >  2:695155/1‑127 (MQ=255)                                                                                                                   gCTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtatg                   <  2:735794/140‑1 (MQ=255)                                                                                                                    cTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                 >  1:233229/1‑125 (MQ=255)                                                                                                                    cTACTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTCAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                 <  2:233229/125‑1 (MQ=255)                                                                                                                       cTCAATGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGc               >  1:151145/1‑140 (MQ=255)                                                                                                                            tGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGc          >  2:723751/1‑140 (MQ=255)                                                                                                                            tGACACAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGc          >  2:333002/1‑140 (MQ=255)                                                                                                                              acacAAATTACCAAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGa        <  1:408611/140‑1 (MQ=255)                                                                                                                               cacaAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGaa       <  1:723751/140‑1 (MQ=255)                                                                                                                                 caAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc     <  1:527236/140‑1 (MQ=255)                                                                                                                                   aaTTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa   <  2:723918/140‑1 (MQ=255)                                                                                                                                    aTTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg  <  1:315931/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG  >  NC_000913/4604545‑4604815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑