Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	415,480	A→G	Y92H (TAC→CAC)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	415,480	0	A	G	100.0%	60.3 / NA	19	Y92H (TAC→CAC)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

TATCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTC  >  NC_000913/415360‑415612                                                                                                                         |                                                                                                                                     tATCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCa                                                                                                                   >  2:216158/1‑140 (MQ=255)              gTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCg                                                                                                      >  2:641326/1‑140 (MQ=255)                                        aGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTc                                                                            >  1:112479/1‑140 (MQ=255)                                            cgcCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATga                                                                        >  1:759506/1‑140 (MQ=255)                                                 gCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCa                                                                   >  1:494761/1‑140 (MQ=255)                                                    ctACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCAttt                                                                >  2:355167/1‑140 (MQ=255)                                                                 cTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGaaa                                                   >  2:751074/1‑140 (MQ=255)                                                                      aaTTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTa                                              >  2:130451/1‑140 (MQ=255)                                                                           ccGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTagagag                                         >  1:718281/1‑140 (MQ=255)                                                                              tCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTaga                                            <  1:179820/134‑1 (MQ=255)                                                                              tCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTaga                                            >  2:179820/1‑134 (MQ=255)                                                                                gggTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGAcgcg                                    <  2:125595/140‑1 (MQ=255)                                                                                   ttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAg                                 <  2:112479/140‑1 (MQ=255)                                                                                   ttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAg                                 <  1:641326/140‑1 (MQ=255)                                                                                        tACGCGCCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCg                            >  2:61256/1‑140 (MQ=255)                                                                                             gcCCGATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATa                       <  1:355167/140‑1 (MQ=255)                                                                                                 gATTCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAgc                   <  1:751074/140‑1 (MQ=255)                                                                                                   ttCTGGCTCCAGAATGCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAgcgc                 <  1:61256/140‑1 (MQ=255)                                                                                                                  gCACGGTGCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc  <  2:718281/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                     TATCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTC  >  NC_000913/415360‑415612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑