Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,173	A→G	Y67H (TAC→CAC)	caiE ←	putative transferase CaiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,173	0	A	G	100.0%	210.2 / NA	60	Y67H (TAC→CAC)	caiE	putative transferase CaiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/28);  total (32/28)

TCTTCGCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTAGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACT  >  NC_000913/35037‑35308                                                                                                                                         |                                                                                                                                        tctTCGCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCGAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGcc                                                                                                                                      <  2:41120/140‑1 (MQ=255) tctTCGCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGcc                                                                                                                                      <  2:797525/140‑1 (MQ=255) tcctCGCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGcc                                                                                                                                      <  2:492997/137‑1 (MQ=255)      gCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCa                                                                                                                                 >  2:791491/1‑140 (MQ=255)           tGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATg                                                                                                                            >  1:487774/1‑140 (MQ=255)                            aTCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATAtt                                                                                                           >  2:687499/1‑140 (MQ=255)                                cGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGcc                                                                                                                       <  1:600963/124‑1 (MQ=255)                                cGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGcc                                                                                                                       >  2:600963/1‑124 (MQ=255)                                cGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCt                                                                                                       >  1:204279/1‑140 (MQ=255)                                cGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCt                                                                                                       >  2:627983/1‑140 (MQ=255)                                  cTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTcc                                                                                                     >  1:666358/1‑140 (MQ=255)                                     ttCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCt                                                                                                       <  1:760225/135‑1 (MQ=255)                                     ttCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCt                                                                                                       >  2:760225/1‑135 (MQ=255)                                     ttCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGc                                                                                                  >  1:663360/1‑140 (MQ=255)                                       cATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCtt                                                                                                <  1:687499/140‑1 (MQ=255)                                          cccGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCa                                                                                             <  1:791491/140‑1 (MQ=255)                                              aCCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGAt                                                                                         <  2:101254/140‑1 (MQ=255)                                               ccAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATc                                                                                        >  1:531569/1‑140 (MQ=255)                                               ccAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATc                                                                                        >  2:777270/1‑140 (MQ=255)                                                         gcgACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGt                                                                              <  1:777270/140‑1 (MQ=255)                                                          cgACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTa                                                                             >  2:347675/1‑140 (MQ=255)                                                             ccAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATCCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCCACCGCCCGTAGTc                                                                          >  2:378743/1‑140 (MQ=255)                                                                aTCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTcacc                                                                       <  2:663360/140‑1 (MQ=255)                                                                aTCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTca                                                                         >  2:135183/1‑138 (MQ=255)                                                                aTCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTca                                                                         <  1:135183/138‑1 (MQ=255)                                                                        acCATGCAGGATCGCTCCGTGCCCGATATGGCCG‑TTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGa                                                              >  1:117130/1‑140 (MQ=255)                                                                           aTGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAgg                                                            >  2:728383/1‑140 (MQ=255)                                                                           aTGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAgg                                                            >  2:595744/1‑140 (MQ=255)                                                                            tGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGc                                                           >  1:653051/1‑140 (MQ=255)                                                                             gCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCg                                                          >  2:146398/1‑140 (MQ=255)                                                                               aGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAg                                                        <  2:487774/140‑1 (MQ=255)                                                                                  aTCGCTCCGTGCCCGATATGGCCG‑TTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    <  2:117130/140‑1 (MQ=255)                                                                                   tCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    <  2:653051/140‑1 (MQ=255)                                                                                   tCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    <  2:531569/140‑1 (MQ=255)                                                                                   tCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    <  2:303777/140‑1 (MQ=255)                                                                                   tCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    >  2:321408/1‑140 (MQ=255)                                                                                   tCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTggg                                                    <  1:595744/140‑1 (MQ=255)                                                                                    cGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGc                                                   >  2:527727/1‑140 (MQ=255)                                                                                     gCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGcc                                                  <  2:666358/140‑1 (MQ=255)                                                                                     gCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGcc                                                  <  2:17051/140‑1 (MQ=255)                                                                                      cTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGa                                                         >  2:317011/1‑132 (MQ=255)                                                                                      cTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGa                                                         <  1:317011/132‑1 (MQ=255)                                                                                      cTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCg                                                 >  2:626977/1‑140 (MQ=255)                                                                                      cTCCGTGCCCGATATCGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCg                                                 >  1:54512/1‑140 (MQ=255)                                                                                               cGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGaca                                        <  1:627983/140‑1 (MQ=255)                                                                                               cGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGaca                                        <  1:248159/140‑1 (MQ=255)                                                                                                     ggCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCt                                  >  1:183869/1‑140 (MQ=255)                                                                                                            ttCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACaa                           >  1:120228/1‑140 (MQ=255)                                                                                                            ttCCCCAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACaa                           >  2:540/1‑140 (MQ=255)                                                                                                               cccAACGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATca                        <  2:120228/140‑1 (MQ=255)                                                                                                                    cGATAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCg                   >  1:281115/1‑140 (MQ=255)                                                                                                                       tAGTGTCAGTGTCGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCa                <  2:54512/140‑1 (MQ=255)                                                                                                                         gtgtcagtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAAt              <  2:111625/140‑1 (MQ=255)                                                                                                                              agtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGa         >  2:676849/1‑140 (MQ=255)                                                                                                                              agtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGa         <  2:183869/140‑1 (MQ=255)                                                                                                                              agtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGa         <  1:540/140‑1 (MQ=255)                                                                                                                               gtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGAc        >  1:708901/1‑140 (MQ=255)                                                                                                                               gtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGAc        >  1:478174/1‑140 (MQ=255)                                                                                                                               gtgtcGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGAc        >  1:129143/1‑140 (MQ=255)                                                                                                                                   cGCAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCa    <  1:347675/140‑1 (MQ=255)                                                                                                                                     cAGTGGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACt  >  2:641010/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TCTTCGCCAATGACCGCGCCATCCATAATCACGCTGTTCATCCCGACCAATGCATCGCGACCAATCAAACAACCATGCAGGATCGCTCCGTGCCCGATATGGCCGTTTTCCCCAACGATAGTGTCAGTGTCGCAGTAGCCATGCATAATGCAGCCATCCTGAATATTGGCTCCCGCTTGCACGATCAACCGCCCGTAGTCACCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACT  >  NC_000913/35037‑35308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑