Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	456,274	C→T	P381L (CCG→CTG)	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	456,274	0	C	T	100.0%	113.5 / NA	33	P381L (CCG→CTG)	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/20);  total (13/20)

CTGTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGA  >  NC_000913/456138‑456409                                                                                                                                         |                                                                                                                                        cTGTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGaa                                                                                                                                      <  2:799154/140‑1 (MQ=255)   gTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAaag                                                                                                                                    <  1:9155/140‑1 (MQ=255)         agaaCAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTa                                                                                                                              <  2:297590/140‑1 (MQ=255)         agaaCAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTa                                                                                                                              <  1:677147/140‑1 (MQ=255)              aGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCg                                                                                                                           <  2:781070/138‑1 (MQ=255)              aGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCg                                                                                                                           >  1:781070/1‑138 (MQ=255)              aGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAg                                                                                                                         >  2:800185/1‑140 (MQ=255)                         cgcgTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCaa                                                                                                              <  2:545387/140‑1 (MQ=255)                               gttgttGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACaa                                                                                                        <  2:3943/140‑1 (MQ=255)                                 tgttgGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAg                                                                                                      <  1:93773/140‑1 (MQ=255)                                   ttgGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGaa                                                                                                    <  1:10669/140‑1 (MQ=255)                                   ttgGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTAC‑GCAAAAACAAAGAAc                                                                                                   <  1:565956/140‑1 (MQ=255)                                    tgGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAAc                                                                                                   <  1:700155/140‑1 (MQ=255)                                     ggCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACt                                                                                                  >  1:60544/1‑140 (MQ=255)                                              ctgGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGacaa                                                                                         <  2:432159/140‑1 (MQ=255)                                                                 ccAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTg                                                                      <  2:320658/140‑1 (MQ=255)                                                                  cAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTgg                                                                     >  1:592682/1‑140 (MQ=255)                                                                     cGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGgaag                                                                  >  1:382213/1‑140 (MQ=255)                                                                          tGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAg                                                             <  2:214397/140‑1 (MQ=255)                                                                          tGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAg                                                             <  2:331798/140‑1 (MQ=255)                                                                               gCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGt                                                        <  2:592682/140‑1 (MQ=255)                                                                               gCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGt                                                        <  2:94457/140‑1 (MQ=255)                                                                                         agCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTa                                              >  2:207685/1‑140 (MQ=255)                                                                                          gcgcGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTAc                                             >  1:709651/1‑140 (MQ=255)                                                                                                   aGGCCTGATCGAAGAGATGGCTTCTGCGTACGCAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGgcgaaag                                    <  2:709651/140‑1 (MQ=255)                                                                                                              aaGAGATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACt                         <  2:618561/140‑1 (MQ=255)                                                                                                               agagATGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTg                        >  2:648553/1‑140 (MQ=255)                                                                                                                    tGGCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGaaaaa                   >  2:300234/1‑140 (MQ=255)                                                                                                                     ggCTTCTGCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAg                  >  2:292047/1‑140 (MQ=255)                                                                                                                            gCGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCAc           >  2:620384/1‑140 (MQ=255)                                                                                                                             cGTACGAAGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACt          >  2:637589/1‑140 (MQ=255)                                                                                                                                   aaGATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAAc    <  1:213699/140‑1 (MQ=255)                                                                                                                                     gATCTGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGa  >  2:379054/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGA  >  NC_000913/456138‑456409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑