Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,275	G→A	S1043S (AGC→AGT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,275	0	G	A	100.0%	146.9 / NA	42	S1043S (AGC→AGT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/17);  total (25/17)

GTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCG  >  NC_000913/481141‑481408                                                                                                                                       |                                                                                                                                      gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACtgtg                                                                                                                                  <  2:263259/140‑1 (MQ=255)         tGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGatat                                                                                                                          >  1:307552/1‑140 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGa                                                                                                                             >  2:68981/1‑135 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGa                                                                                                                             <  2:515116/135‑1 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGa                                                                                                                             >  1:515116/1‑135 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGa                                                                                                                             <  1:68981/135‑1 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCt                                                                                                                        >  2:321527/1‑140 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCt                                                                                                                        >  2:254230/1‑140 (MQ=255)           gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCt                                                                                                                        >  2:122520/1‑140 (MQ=255)                                   gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg                                                                                                >  2:771561/1‑140 (MQ=255)                                   gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg                                                                                                >  2:734343/1‑140 (MQ=255)                                   gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg                                                                                                >  2:537473/1‑140 (MQ=255)                                   gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg                                                                                                >  1:128605/1‑140 (MQ=255)                                               cTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca                                                                                    <  1:734343/140‑1 (MQ=255)                                               cTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca                                                                                    <  1:537473/140‑1 (MQ=255)                                                 ccTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa                                                                                  >  1:596914/1‑140 (MQ=255)                                                              aGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACg                                                                     >  1:113714/1‑140 (MQ=255)                                                               ggTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACga                                                                    <  2:113714/140‑1 (MQ=255)                                                                   aTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa                                                                >  2:523240/1‑140 (MQ=255)                                                                    tGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag                                                               <  2:367329/140‑1 (MQ=255)                                                                           aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCa                                                        >  2:54599/1‑140 (MQ=255)                                                                                gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAcc                                                   <  2:642092/140‑1 (MQ=255)                                                                                  cGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga                                                              >  1:373064/1‑127 (MQ=255)                                                                                  cGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga                                                              <  2:373064/127‑1 (MQ=255)                                                                                   gCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                <  1:523240/140‑1 (MQ=255)                                                                                              cTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCAt                                     >  2:527205/1‑140 (MQ=255)                                                                                                 aGTGATTACACGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATccc                                  <  2:627339/140‑1 (MQ=255)                                                                                                           cGTTGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                        <  2:219691/140‑1 (MQ=255)                                                                                                             ttGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                               <  2:38164/131‑1 (MQ=255)                                                                                                             ttGTATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                               >  1:38164/1‑131 (MQ=255)                                                                                                                tATCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGt                   <  1:254230/140‑1 (MQ=255)                                                                                                                  tCAATGATGATCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                 <  2:671610/140‑1 (MQ=255)                                                                                                                        atgatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTg           >  2:386662/1‑140 (MQ=255)                                                                                                                          gatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                        <  2:662325/125‑1 (MQ=255)                                                                                                                          gatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                        >  1:662325/1‑125 (MQ=255)                                                                                                                          gatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg         >  1:442808/1‑140 (MQ=255)                                                                                                                          gatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg         >  1:119937/1‑140 (MQ=255)                                                                                                                          gatCGACAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg         >  2:145267/1‑140 (MQ=255)                                                                                                                                cAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc   >  2:641413/1‑140 (MQ=255)                                                                                                                                cAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc   <  2:307552/140‑1 (MQ=255)                                                                                                                                cAGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc   >  1:726443/1‑140 (MQ=255)                                                                                                                                 aGTATGACTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg  >  2:477081/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCG  >  NC_000913/481141‑481408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑